A5115687694- S100 Proteins and Annexins
- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- Endoplasmic Reticulum Stress and Disease
- RNA modifications and cancer
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Cancer, Lipids, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Protease and Inhibitor Mechanisms
- Traumatic Brain Injury and Neurovascular Disturbances
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Heat shock proteins research
- Pancreatic function and diabetes
- Healthcare and Venom Research
- Cell Image Analysis Techniques
- Cancer Immunotherapy and Biomarkers
- Cancer Cells and Metastasis
- Connexins and lens biology
- Extracellular vesicles in disease
- Ubiquitin and proteasome pathways
- Mesenchymal stem cell research
- Phytochemistry and Bioactivity Studies
- Acute Kidney Injury Research
Nanjing Medical University
2015-2024
Wenzhou Medical University
2022
Second Affiliated Hospital of Nanjing Medical University
2015-2021
Nanjing Jiangning Hospital
2021
Jiangsu Province Hospital
2021
University of North Texas
2017
Children's Hospital of Pittsburgh
2009
The University of Texas Health Science Center at Tyler
2007
Iowa State University
1994
OBJECTIVE Macrophages play an important role in the pathogenesis of insulin resistance via production proinflammatory cytokines. Our goal is to decipher molecular linkage between cytokine and macrophages. RESEARCH DESIGN AND METHODS We determined profiles cultured macrophages identified interleukin (IL)-1β gene as a potential target FoxO1, key transcription factor that mediates action on expression. studied mechanism by which FoxO1 insulin-dependent regulation IL-1β expression correlated...
In mammals, resting primordial follicles serve as the ovarian reserve. The decline in function with aging is characterized by a gradual decrease both quantity and quality of oocytes residing within follicles. Many reports show that mesenchymal stem cells have ability to recover premature insufficiency (POI) or natural animal models; however, underlying mechanism remains unclear. this study, using exosomes derived from human umbilical cord (HucMSC-exos), we found specific accumulation...
Ganoderma lucidum polysaccharides (GLPS) have been used as traditional Chinese medicine for their properties of cancer prevention and immunomodulation. However, it is unclear whether GLPS has therapeutic effect on anti-hepatocellular carcinoma (HCC) in vivo. In this study, the impact balance regulatory T cell (Treg) effector (Teff) was measured a model hepatoma-bearing mice.The were mice. Real-time PCR detected levels MicroRNAs (miRNAs) mRNA. The effects Tregs Teff proliferation determined...
Abstract Background E3 ubiquitin ligase HRD1 (HMG-CoA reductase degradation protein 1, alias synoviolin with SYVN1 as the official gene symbol) was found downregulated and acting a tumor suppressor in breast cancer, while exact expression profile of different cancer subtypes remains unknown. Recent studies characterized circular RNAs (circRNAs) playing an regulatory role miRNA sponge progression, presenting new viewpoint for post-transcriptional regulation cancer-related genes. Methods...
Recently, long non-coding RNA (lncRNA) MIAT has been demonstrated as an oncogenic gene in several types of cancer. However, the role and mechanism colorectal cancer (CRC) have not investigated.Real-time PCR was used to measure expression CRC tissues cells. Small interfering specific for (si-MIAT) down-regulate The interaction miR-132 measured by pull-down assay. effect si-MIAT on cells apoptosis metastasis were flow cytometry assay, invasion migration respectively.In present study, we found...
S100A16 is a member of the S100 calcium-binding protein family. It overexpressed in many types tumors and associated with proliferation, migration, invasion; however, its function human prostate cancer unresolved. Our objective was to determine effects underlying pathways tissues cells. We measured expression by quantitative real-time polymerase Western blotting eight matched adjacent normal tissues, three cell lines, DU-145, LNCaP, PC-3, compared epithelial line PrEC. DU-145 cells stably...
Apoptosis of renal tubular epithelial cells is a key feature the pathogenicity associated with tubulointerstitial fibrosis and other kidney diseases. One factor that regulates important cellular processes like apoptosis cell proliferation HRD1, an E3 ubiquitin ligase acts by promoting ubiquitylation degradation its target protein. However, detailed mechanisms which HRD1 as regulator in have not been established. In our previous liquid chromatography-tandem mass spectrometry (LC-MS/MS) study...
Head and neck squamous cell carcinoma (HNSCC) is the fifth most common cancer worldwide a cause of cancer-related death, with 5-year survival rate less than 60%. IL-6 has been suggested to play an important role in metastasis, but its mechanism HNSCC not fully clarified. p70S6K reported induce epithelial-mesenchymal transition (EMT) ovarian cancer, remains unknown. In this study, we found that were upregulated high-metastatic lines underwent EMT when compared paired low-metastatic lines....
Cancer susceptibility candidate 2 (CASC2), a long non-coding RNA (lncRNA), has been demonstrated to be tumor suppressor in several types of cancer. However, the role and mechanism CASC2 breast cancer (BC) have not investigated. In present study, expression functions BC were The was significantly decreased tissues cells compared with adjacent normal mammary epithelial cells, respectively. overexpression inhibited viability, migration invasion, elevated apoptosis cells. addition, acted as...
// Yue-Mei Xu 1, * , Hong-Jiang Wang 2, Fang Chen 3, Wan-Hua Guo 4, Yan-Yang Hang-Yu Li 5, Jin-Hai Tang 6, Ying Ding 1 Ya-Chen Shen 7 Min Wen-Ying Xuan Lin-Hui Liu Jia 2 Xue-Rong Ze-Jun Gao Xiu-Bin Liang Dong-Ming Su 7, 8 State Key Laboratory of Reproductive Medicine, Department Pathology, Nanjing Medical University, Nanjing, China Breast Surgery, First Affiliated Hospital Dalian Dalian, 3 Human Functional Genomics Jiangsu Province, 4 Nuclear The Drum Tower 5 General 4th Hospital, Shenyang,...
BackgroundExtracellular heat shock protein 70 and peptide complexes (eHSP70/HSP70-PCs) regulate a variety of biological behaviors in tumor cells. Whether eHSP70/HSP70-PCs are involved the epithelial-mesenchymal transition (EMT) cells remains unclear. AimsTo determine effects on EMT hepatocarcinoma MethodsThe expressions E-cadherin, HSP70, α-smooth muscle actin (α-SMA) p-p38 were detected immunohistochemically liver cancer samples. Immunofluorescence, western blotting real-time RT-PCR methods...
The pathogenesis of acute kidney injury (AKI) is associated with the activation multiple signaling pathways, including Wnt/β-catenin signaling. However, mechanism pathway in renal interstitial fibroblasts during AKI unclear. S100 calcium-binding protein A16 (S100A16), a new member family, multi-functional factor involved various pathogenies, tumors, glycolipid metabolism disorder, and chronic disease (CKD). We investigated potential participation S100A16 by subjecting wild-type (WT) knockout...
Heat shock protein 70 (HSP70) and HSP70-peptide complexes (HSP70-PCs) have been implicated in the pathogenesis of multiple tumors humans experimentally shown to increase proliferation cell lines derived from hepatocellular carcinoma. The goal this study was elucidate molecular mechanisms through which extracellular HSP70/HSP70-PCs stimulate carcinoma (HCC). HSP70/HSP70-PC action were studied human HepG2 Huh-7, as well tumor tissue collected patients with HCC (n = 95). We found that can cells...
Injury of renal tubular epithelial cells is a key feature the pathogenicity associated with tubulointerstitial fibrosis and other kidney diseases. HUWE1, an E3 ubiquitin ligase, acts by participating in ubiquitination degradation its target proteins. However, detailed mechanisms which HUWE1 might regulate have not been established. Here, possible regulation was investigated examining expression EGFR unilateral ureteral obstruction (UUO) mice. Markedly consistent reciprocal changes were...
Abstract Background Our previous studies have shown that the E3 ubiquitin ligase of HMG-CoA reductase degradation 1 (HRD1) functions as a tumor suppressor, overexpression HRD1 suppressed breast cancer proliferation and invasion. However, its role in cell glucose metabolism was unclear. Here, our aim to uncover molecular mechanisms regulating aerobic glycolysis cancer. Methods The effect on robic cells were assessed. Then proliferation, colony formation ability, invasion migration evaluated....
Studies revealed an important role of microRNAs (miRNAs) in multiple cancers, including breast cancer. In the present study, we evaluated and function miR-641 cancer.The expression level cancer cell lines (Hs-578T, MCF7, HCC1937, MAD-MB-231) was determined by real-time PCR. Functional analyses, CCK-8 assay, transwell wound-healing apoptosis detection, were carried out to explore roles miRNA-641 malignant behaviors Luciferase report assay used investigate regulatory association with its...
Ubiquitylation of the epithelial Na+ channel (ENaC) plays a critical role in cellular functions, including transmembrane transport Na+, and water balance, blood pressure stabilization. Published studies have suggested that ENaC subunits are targets ER-related degradation (ERAD) yeast systems. However, molecular mechanism underlying proteasome-mediated remains to be established. Derlin-1, an E3 ligase mediator, links recognized target proteins ubiquitin-mediated proteasomal cytosol. In...
Abstract Renal fibrosis arises by the generation of matrix-producing fibroblasts and myofibroblasts through epithelial–mesenchymal transition (EMT), a process in which epithelial cells undergo into fibroblast phenotype. A key feature EMT is reorganization cytoskeletons, may involve Ca 2+ -binding protein S100A16, newly reported member S100 family. However, very few studies have examined role S100A16 renal tubulointerstitial fibrosis. In this study, expression was immunohistochemical staining...
The 3-hydroxy-3-methylglutaryl reductase degradation (HRD1) is an E3 ubiquitin ligase that can preserve heart structure and function, but its role in endothelial dysfunction atherosclerosis (AS) unclear. aim of this study was to explore the biological function HRD1 AS. expression significantly decreased atherosclerotic intima ox-LDL led a decrease level cells (ECs). Forced inhibited apoptosis induced by ox-LDL. transcription factor KLF2 specifically bound promoter positively regulated...