A5010479124- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Viral Infections and Vectors
- Vector-borne infectious diseases
- Leptospirosis research and findings
- Parasites and Host Interactions
- Vector-Borne Animal Diseases
- Insect Pest Control Strategies
- Parasitic Diseases Research and Treatment
- Parasitic Infections and Diagnostics
- Insects and Parasite Interactions
- Mosquito-borne diseases and control
- Herpesvirus Infections and Treatments
- Toxin Mechanisms and Immunotoxins
- Synthesis and Biological Evaluation
- Metal complexes synthesis and properties
- Ferrocene Chemistry and Applications
- Crystallization and Solubility Studies
- Virus-based gene therapy research
- Intimate Partner and Family Violence
- Cynara cardunculus studies
- Business and Management Studies
- X-ray Diffraction in Crystallography
- Insect symbiosis and bacterial influences
- Mollusks and Parasites Studies
Universidade Nova de Lisboa
2013-2022
Instituto de Medicina Tropical
2001-2019
University of Algarve
2011-2017
Instituto de Investigação Científica Tropical
1991-2016
University of Lisbon
2015-2016
Background The recent geographical expansion of phlebotomine vectors Leishmania infantum in the Mediterranean subregion has been attributed to ongoing climate changes. At these latitudes, activity sand flies is typically seasonal; because seasonal phenomena are also sensitive general variations climate, current phenological data sets can provide a baseline for continuing investigations on fly population dynamics that may impact future scenarios leishmaniasis transmission. With this aim,...
Feline vector-borne diseases (FVBD) have emerged in recent years, showing a wider geographic distribution and increased global prevalence. In addition to their veterinary importance, domestic cats play central role the transmission cycles of some FVBD agents by acting as reservoirs sentinels, circumstance that requires One Health approach. The aim present work was molecularly detect feline bacteria protozoa with zoonotic assess associated risk factors from southern Portugal. Six hundred...
This study was performed in order to test the efficacy of a new polymerase chain reaction (PCR) assay for diagnosis both human and canine leishmaniasis caused by Leishmania infantum. The primers were developed on basis complete DNA sequence L. infantum kinetoplast minicircle. Specificity sensitivity evaluated testing bone marrow spots filter paper skin biopsy samples, PCR results compared data from vitro cultures. strains different foci, as well other trypanosomatids opportunistic pathogenic...
We compared the validity of pancytopenia, formol-gel test (FGT), indirect fluorescence antibody (IFAT), direct agglutination (DAT), and rK39 dipstick as diagnostic criteria for visceral leishmaniasis (VL) in Nepal. Between September 2000 January 2002, 310 clinical suspects had a bone marrow aspirate, if negative, spleen aspirate smear examined Leishmania donovani. Sensitivity specificity all tests were determined with parasitology by latent class analysis (LCA). Compared parasitology,...
Summary Seven mixed‐breed dogs were challenged with either promastigotes or amastigotes of Leishmania donovani infantum strains recently isolated from naturally infected dogs. Different routes and numbers parasites utilized each dog was monitored for at least 1 year post‐infection. Anti‐parasite specific antibody levels measured by enzyme‐linked immunosorbence, immunofluorescence, crossed‐immune electrophoresis Western blotting on crude antigen. two pure parasite proteins, dp72 gp70‐2. also...
Leishmaniasis, caused by Leishmania infantum, is an endemic zoonosis in the Mediterranean basin. Dogs are considered major host for these parasites, as well main reservoir human visceral infection. In recent years, asymptomatic infection or clinical disease L. infantum cats has been reported several countries where zoonotic leishmaniasis present. The aim of present study was to perform a survey from focus. Twenty-three adult stray were surveyed examination, and peripheral blood samples...
BackgroundLeishmania infantum is the causative agent of visceral and cutaneous leishmaniasis in Mediterranean region, South America, China. MON-1 L. predominating zymodeme all endemic regions, both humans dogs, reservoir host. In order to answer important epidemiological questions it essential discriminate strains MON-1.Methodology/Principal FindingsWe have used a set 14 microsatellite markers analyse 141 mainly from Spain, Portugal, Greece which 107 were typed by MLEE as MON-1. The highly...
Phlebotomine sandflies of the genus Sergentomyia are widely distributed throughout Old World. It has been suggested that spp involved in transmission Leishmania India and Africa, whereas Phlebotomus thought to be sole vectors In this study, major DNA was detected one minuta specimen collected southern region Portugal. This study challenges dogma is exclusively transmitted by species
Leishmaniasis has reemerged in recent years showing a wider geographic distribution and increased global incidence of human canine disease than previously known. Dogs are the main domestic/peridomestic reservoir hosts zoonotic visceral leishmaniasis caused by Leishmania infantum. Since evolution clinical appearance is consequence complex interactions between parasite host immune response, profound knowledge about profile developed dog's infection crucial for vaccine immunomodulatory therapy...
Zoonotic visceral leishmaniasis caused by Leishmania infantum which is transmitted phlebotomine sand flies (Diptera, Psychodidae) endemic in the Mediterranean basin. The main objectives of this study were to (i) detect DNA and (ii) identify blood meal sources wild caught female zoonotic region Algarve, Portugal/Southwestern Europe. Phlebotomine collected using CDC miniature light traps sticky papers. Sand identified morphologically tested for sp. PCR ITS-1 as target sequence. source engorged...
Most of the experimental studies Leishmania spp. infection require determination parasite load in different tissues. Quantification parasites by microscopy is not very sensitive and time consuming, whereas culture microtitrations remain laborious can be jeopardized microbial contamination. The aim this study was to quantify infantum real-time polymerase chain reaction (PCR) using specific DNA TaqMan® probes compare efficacy detection technique with a PCR–enzyme-linked immunosorbent assay...
Until the early 1990s, pentavalent antimony was only documented first-line drug employed for treatment of zoonotic visceral leishmaniasis (VL) in Mediterranean, with reported cure rates exceeding 95% immunocompetent patients. The emergence resistance other endemic settings and increase options have stimulated re-evaluation current therapeutic approaches outcomes Mediterranean countries. A scientific consortium ('LeishMed' network) collected updated information from collaborating clinical...