Rheumatoid arthritis is an inflammatory joint disease that can lead to pain, stiffness, and deformity, often with marked involvement of the small joints foot ankle. Orthotic devices are commonly prescribed for this condition lessen symptoms improve function mobility, customized most effective. The work reported in paper has examined feasibility using additive manufacturing-based approach manufacture orthoses. In order test feasibility, orthoses have been manufactured manufacturing technology...

The design of foot and ankle orthoses is currently limited by the methods used to fabricate devices, particularly in terms geometric freedom potential include innovative new features. Additive manufacturing (AM) technologies, where objects are constructed via a series sub-millimetre layers substrate material, may present opportunity overcome these limitations allow novel devices be produced that highly personalised for individual, both fit functionality. Two orthosis (FO) designed adjustable...

Hydrogels based on natural polymers offer a range of properties to mimic the native extracellular matrix, and provide microenvironments preserve cellular function encourage tissue formation. A tri-component hydrogel using collagen, alginate fibrin (CAF) was developed investigated at three collagen concentrations for application as functional matrix analogue. Physical-chemical characterization CAF hydrogels demonstrated thermo-responsive crosslinking capacity physiological conditions with...

The use of composite materials, processed as 3D tissue-like scaffolds, has been widely investigated a promising strategy for bone tissue engineering applications. Also, additive manufacturing technologies such fused deposition modelling (FDM) have greatly contributed to the manufacture patient-specific scaffolds with predefined pore structures and intricate geometries. However, conventional FDM techniques require materials exclusively in form filaments, which order produce lead additional...

Bone tissue engineering has emerged as a promising strategy to overcome the limitations of current treatments for bone-related disorders, but trade-off between mechanical properties and bioactivity remains concern many polymeric materials. To address this need, novel blends poly-L-lactic acid (PLLA), polycaprolactone (PCL) poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) have been explored. Blend filaments comprising PLLA/PCL/PHBV at ratio 90/5/5 wt% prepared using twin-screw extrusion....

Bone scaffolds must have an appropriate porosity range with interconnected and open porosity, a biodegradable rate mechanical properties that match the injured tissue. The use of commercially available low cost 3D printing machines offers ability to fulfil these requirements. Therefore, one aims this study was manufacture PLA scaffold by two-step route: 1) porous bar 2) laser cut from printed bar. Laser cutting did not cause any significant degradation issues scanning electron microscopy...

Spinal fusion is the gold standard surgical procedure for degenerative spinal conditions when conservative therapies have been unsuccessful in rehabilitation of patients. Novel strategies are required to improve biocompatibility and osseointegration traditionally used materials lumbar cages. Furthermore, new design technologies needed bridge gap due shortage optimal implant sizes fill intervertebral disc defect. Within this context, additive manufacturing technology presents an excellent...

Purpose The purpose of this paper is to study the effects particle size distribution, component ratio, packing arrangement, and chemical constitution on laser sintering behaviour blended hypoeutectic Al‐Si powders. Design/methodology/approach A range bimodal trimodal powder blends were created through mixing Al‐12Si pure aluminium powder. then processed using selective investigate effect alloy composition, bed density densification microstructural evolution. Findings For all sintered...

Abstract Articular cartilage (AC) lacks the ability to self-repair and cell-based approaches, primarily based on using chondrocytes mesenchymal stem cells (MSCs), are emerging as effective technology restore functionality, because synergic functionality may support maintenance of chondrogenic phenotype promote extracellular matrix regeneration. This work aims develop a more physiologically representative co-culture system investigate influence MSCs activity chondrocytes. A thermo-sensitive...

PLLA, PCL and PHBV are aliphatic polyesters which have been researched used in a wide range of medical devices, all three advantages disadvantages for specific applications. Blending these materials is an attractive way to make material overcomes the limitations individual polymers. Both evaluated polymer blends with PLLA order provide enhanced properties This paper explores use together ternary assessment thermal, mechanical processing resultant blends, aim producing new biomaterials...

Cell seeding via cell-laden hydrogels offers a rapid way of depositing cells onto substrate or scaffold. When appropriately formulated, provide dense network fibres for cellular encapsulation and attachment, creating protective environment that prevents to be washed away by media. However, when incorporating into cell strategy the capacity migration from hydrogel subsequent biofunctionality must assessed. Here, we compare bioprinted with conventional manual in To this end, use binder jet 3D...

The feasibility of using indirect selective laser sintering (SLS) to produce parts from glass-ceramic materials for bone replacement applications has been investigated. A castable glass based on the system SiO 2 Al O 3 P 5 CaO CaF that crystallizes a with apatite and mullite phases was produced, blended an acrylic binder, processed by SLS. Green good structural integrity were produced wide range processing conditions, allowing both monolayer multilayer components be constructed. Following...

There is a clinical need for new bone replacement materials that combine long implant life with complete integration and appropriate mechanical properties. We have used human mesenchymal stem cells (MSCs) to populate porous apatite-wollastonite (A-W) glass-ceramic scaffolds produced by the layer manufacturing technique, selective laser sintering, create custom-built replacements. Confocal scanning electron microscopy were determine optimal seeding densities demonstrate MSCs adhered retained...

Abstract Composites offer the option of coupling individual benefits their constituents to achieve unique material properties, which can be extra value in many tissue engineering applications. Strategies combining hydrogels with fibre-based scaffolds create constructs enhanced biological and structural functionality. However, developing efficient scalable approaches manufacture such composites is challenging. Here, we use a droplet-based bioprinting system called reactive jet impingement...

The in vitro evaluation of 3D scaffolds for bone tissue engineering mono-cultures is a common practice; however, it does not represent the native complex nature tissue. Co-cultures osteoblasts and osteoclasts, without addition stimulating agents monitoring cellular cross-talk, remains challenge. In this study, growth factor-free co-culture human marrow-derived mesenchymal stem cells (hBM-MSCs) peripheral blood mononuclear (hPBMCs) has been established used 3D-printed engineering. were...

Advances in three-dimensional cell cultures offer new opportunities biomedical research and drug development. However, there are still challenges to overcome, including the lack of reliability, repeatability complexity tissues obtained by these techniques. In this study, we describe a bioprinting system called reactive jet impingement (ReJI) for cell-laden hydrogels. Droplets gel precursor solutions jetted at one another such that they meet react mid-air before droplets fall substrate. This...

Ionic substitutions have been proposed as a tool to control the functional behavior of synthetic hydroxyapatite (HA), particularly for Bone Tissue Engineering applications. The effect simultaneous substitution different levels carbonate (CO3 ) and silicon (Si) ions in HA lattice was investigated. Furthermore, human bone marrow-derived mesenchymal stem cells (hMSCs) were cultured on multi-substituted (SiCHA) determine if biomimetic chemical compositions osteoconductive. Of four investigates,...