A5003151499- Single-cell and spatial transcriptomics
- Hematopoietic Stem Cell Transplantation
- Cancer Genomics and Diagnostics
- Acute Myeloid Leukemia Research
- Zebrafish Biomedical Research Applications
- Axon Guidance and Neuronal Signaling
- Immune cells in cancer
- Cell Image Analysis Techniques
- Neurogenesis and neuroplasticity mechanisms
- Immunotherapy and Immune Responses
- Biosensors and Analytical Detection
- Mesenchymal stem cell research
- Gene Regulatory Network Analysis
- Immune Cell Function and Interaction
Heidelberg Institute for Stem Cell Technology and Experimental Medicine
2018-2022
DKFZ-ZMBH Alliance
2018-2022
German Cancer Research Center
2018-2022
Heidelberg University
2018-2022
Cancer Research Center
2018
Abstract Single-cell genomics technology has transformed our understanding of complex cellular systems. However, excessive cost and a lack strategies for the purification newly identified cell types impede their functional characterization large-scale profiling. Here, we have generated high-content single-cell proteo-genomic reference maps human blood bone marrow that quantitatively link expression up to 197 surface markers identities biological processes across all main hematopoietic in...
Cancer stem cells drive disease progression and relapse in many types of cancer. Despite this, a thorough characterization these remains elusive with it the ability to eradicate cancer at its source. In acute myeloid leukemia (AML), leukemic (LSCs) underlie mortality but are difficult isolate due their low abundance high similarity healthy hematopoietic (HSCs). Here, we demonstrate that LSCs, HSCs, pre-leukemic can be identified molecularly profiled by combining single-cell transcriptomics...
Residing at the top of hematopoietic hierarchy, long-term stem cells (HSCs) are capable self-renewal and sustained blood cell regeneration. Over past decades, single-cell clonal analyses have revealed substantial functional molecular heterogeneity within this compartment, challenging notion that is inherently tied to balanced, multi-lineage production. However, a cohesive model explains relationships among these diverse HSC states remains elusive. Here, we combined transplantations over...
Thalamocortical axons (TCAs) cross several tissues on their journey to the cortex. Mechanisms must be in place along route ensure they connect with targets an orderly fashion. The ventral telencephalon acts as instructive tissue, but importance of diencephalon TCA mapping is unknown. We report that disruption diencephalic development by Pax6 deletion results a thalamocortical projection containing errors. used conditional mutagenesis test whether these errors are due pioneer projections from...
ABSTRACT Single-cell genomics has transformed our understanding of complex cellular systems. However, excessive costs and a lack strategies for the purification newly identified cell types impede their functional characterization large-scale profiling. Here, we have generated high content single-cell proteo-genomic reference maps human blood bone marrow that quantitatively link expression up to 197 surface markers identities biological processes across all major hematopoietic in healthy...
SUMMARY The bone marrow (BM) constitutes the primary site for life-long blood production and skeletal regeneration. However, its cellular composition spatial organization into distinct ‘niches’ remains controversial. Here, we combine single-cell spatially resolved transcriptomics to systematically map molecular of endosteal, sinusoidal, arteriolar BM niches. This allowed us transcriptionally profile all major resident cell types, determine their localization, clarify sources key growth...
Abstract Cancer stem cells drive disease progression and relapse in many types of cancer. Despite this, a thorough characterization these remains elusive with i the ability to eradicate cancer at its source. In acute myeloid leukemia (AML), leukemic (LSCs) underlie mortality but are difficult isolate due their low abundance high similarity healthy hematopoietic (HSCs). Here, we demonstrate that LSCs, HSCs, pre-leukemic can be identified molecularly profiled by combining single-cell...
Abstract Thalamocortical axons (TCAs) cross several tissues on their journey to the cortex. Mechanisms must be in place along route ensure they connect with targets an orderly fashion. The ventral telencephalon acts as instructive tissue, but importance of diencephalon TCA mapping is unknown. We report that disruption diencephalic development by Pax6 deletion results a thalamocortical projection containing errors. used conditional mutagenesis test whether these errors are due pioneer...