1. The N-acetyl-β-glucosaminidase of human spleen has been separated by gel electrophoresis into two components, an acidic form A and a basic B. 2. forms are readily on DEAE-cellulose have concentrated 50-fold sevenfold respectively. 3. They show similar Km values towards 4-methylumbelliferyl N-acetyl-β-d-glucosaminide, the same pH optima when compared in citrate, phosphate or acetate buffers. inhibited to extent acetate, heparin, N-acetylgalactosaminolactone, N-acetyl-β-d-galactosamine...

The dynamic collision behavior of the electro-oxidation single Ag nanoparticles is observed at Au microelectrodes using stochastic single-nanoparticle amperometry. Results show that an nanoparticle collision/oxidation event typically consists a series 1 to ∼10 discrete "sub-events" over ∼20 ms interval. also undergo only partial oxidation prior diffusing away from electrode into bulk solution. Both behaviors are characterized and shown exist under variety experimental conditions. These...

Research Article| February 01 1967 Separation and properties of β-galactosidase, β-glucosidase, β-glucuronidase N-acetyl-β-glucosaminidase from rat kidney D Robinson; Robinson Search for other works by this author on: This Site PubMed Google Scholar RG Price; Price N Dance Biochem J (1967) 102 (2): 525–532. https://doi.org/10.1042/bj1020525 Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Facebook Twitter LinkedIn MailTo Cite Get Permissions...

Here we describe a very simple, reliable, low-cost electrochemical approach to detect single nanoparticles (NPs) and evaluate NP size distributions catalytic activity in fast reproducible manner. Single NPs are detected through an increase current caused by electrocatalytic oxidation of N(2)H(4) at the surface when it contacts Hg-modified Pt ultramicroelectrode (Hg/Pt UME). Once Hg/Pt UME, Hg poisons NP, deactivating reaction. Hence, response is "spike" that decays background level rather...

Research Article| May 01 1956 The fluorimetric determination of β-glucosidase: its occurrence in the tissues animals, including insects D. Robinson 1Department Biochemistry, St Mary's Hospital Medical School, London, W. 2 Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1956) 63 (1): 39–44. https://doi.org/10.1042/bj0630039 Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Facebook Twitter LinkedIn Email Cite Get...

A new type of gold nanoparticle with interesting energetics has been created by employing a mixture dithiol durene (Durene-DT) and monothiol phenylethanethiol (PhC2S) in the synthesis. The average composition these mixed thiolate clusters is characterized to be Au(130)(Durene-DT)(29)(PhC2S)(22). Continuous quantized core charging behaviors were observed at lower potentials voltammetric measurements, while ligand reaction core-ligand interactions higher potentials. absorbance spectrum...

Recent high-bandwidth recordings of the oxidation and dissolution 35 nm radius Ag nanoparticles at a Au microelectrode show that these undergo multiple collisions with electrode, generating electrochemical current peaks. In time interval between observed peaks, diffuse in solution near electrolyte/electrode interface. Here, we demonstrate simulations random nanoparticle motion, coupled kinetic parameters, quantitatively reproduce experimentally multicurrent peak behavior. Simulations...

Non-von-Neumann computing using neuromorphic systems based on two-terminal resistive nonvolatile memory elements has emerged as a promising approach, but its full potential not been realized due to the lack of materials and devices with appropriate attributes. Unlike memristors, which require large write currents drive phase transformations or filament growth, electrochemical random access (ECRAM) decouples "write" "read" operations "gate" electrode tune conductance state through...

Normal calf α-mannosidase activity exists in at least three forms separable by chromatography on DEAE-cellulose and starch-gel electrophoresis. Two components, A B, have optimum between pH3.75 4.75, but component C has an of pH6.6. Components B are virtually absent from the tissues a with mannosidosis residual is due to C. The acidic neutral differ their molecular weights sensitivity EDTA, Zn2+, Co2+ Mn2+. An (pH 4.0) accounts for most normal plasma it mannosidosis. Although probably related...

1. Ten rat tissues and organs have been assayed for beta-galactosidase with phenyl beta-d-galactoside, p-nitrophenyl p-aminophenyl beta-d-galactoside 4-methylumbelliferyl as substrates. 2. The relative activities of these are independent the mode assay, maximum rates hydrolysis not greatly affected by nature substrate. 3. Inhibition studies suggest liver enzyme has no associated beta-glucosidase activity. 4. There is cellular localization preferential activity towards any four substrates in...

Research Article| April 01 1958 Studies in detoxication. 74. The metabolism of benzhydrol, benzophenone and p-hydroxybenzophenone* D. Robinson 1Department Biochemistry, St Mary's Hospital Medical School, London, W. 2 Search for other works by this author on: This Site PubMed Google Scholar Author article information Publisher: Portland Press Ltd © CAMBRIDGE UNIVERSITY PRESS1958 Biochem J (1958) 68 (4): 584–586. https://doi.org/10.1042/bj0680584 Views Icon Article contents Figures & tables...

Research Article| January 01 1953 Studies in detoxication. 45. β-Glucuronidase and arylsulphatase the crop fluid of locusts D. Robinson; Robinson 1Department Biochemistry, St Mary's Hospital Medical School, London, W. 2 Search for other works by this author on: This Site PubMed Google Scholar J. N. Smith; Smith R. T. Williams Biochem J (1953) 53 (1): 125–129. https://doi.org/10.1042/bj0530125 Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review Share...

We show that the deposition of solid-state electrolyte LiPON onto films V2O5 leads to their uniform lithiation up 2.2 Li per V2O5, without affecting concentration in and its ionic conductivity. Our results indicate incorporation occurs during deposition, contrast earlier mechanisms proposed explain postdeposition transfer between LiCoO2. use our discovery demonstrate symmetric thin film batteries with a capacity >270 mAh/g, at rate 20C, 1600 cycles only 8.4% loss capacity. also how...

Antisera were raised to a partially purified preparation of human liver hexosaminidase and highly preparations isoenzymes A B. All the antisera precipitated enzyme in an enzymically active form, which could be located on immunodiffusion immunoelectrophoretic gels by using histochemical substrate. The shown react with from liver, kidney, brain spleen, but did not cross-react beta-glucosidase, beta-galactosidase, alpha-mannosidase, beta-xylosidase, arylsulphatase or acid phosphatase....

Human liver acidic alpha-D-mannosidase was purified 1400-fold by a relatively short procedure incorporating chromatography on concanavalin A-Sepharose and affinity Sepharose 4B-epsilon-aminohexanoylmannosylamine. In contrast with the enzymic activity neutral alpha-mannosidase did not bind to so two types of could be separated at an early stage in purification. The only significant glycosidase contaminant after mannosylamine ligand alpha-L-fucosidase, which selectively removed corresponding...

1. GM(1)-ganglioside, specifically tritiated in the terminal galactose, was hydrolysed by two forms of ;acid' methylumbelliferyl beta-galactosidase isolated on gel filtration. 2. Identification GM(1)-ganglioside activity with methyl-umbelliferyl beta-galactosidases based following: coincident elution profiles filtration; simultaneous inactivation heat and other treatments; stabilization both activities chloride ions; mutual inhibition hydrolysis substrates. 3. The isoenzymes (I) (II) showed...

Here, we report a potentiometric method for detecting single platinum nanoparticles (Pt NPs) by measuring change in open-circuit potential (OCP) instead of the current during Pt NP collisions with mercury-modified ultramicroelectrode (Hg/Pt UME). Similar to current-time (i-t) response reported previously at Hg/Pt UMEs, OCP-time (v-t) consists repeated transient signals that return background level. This is because Hg poisons after collision UME due amalgamation and results deactivation redox...

Research Article| February 01 1967 Rat-urine glycosidases and kidney damage D Robinson; Robinson Search for other works by this author on: This Site PubMed Google Scholar RG Price; Price N Dance Biochem J (1967) 102 (2): 533–538. https://doi.org/10.1042/bj1020533 Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Facebook Twitter LinkedIn MailTo Cite Get Permissions Citation Robinson, Price, Dance; damage. 1 1967; doi: Download citation file: Ris...

Herein the problem of colloidal instability on electrochemically detected nanoparticle (NP) collisions with a Hg ultramicroelectrode (UME) by electrocatalytic amplification is addressed. NP tracking analysis (NTA) shows that rapid aggregation occurs in solution after diluting citrate-stabilized Pt NPs hydrazine/phosphate buffers net ionic strength greater than 70 mM. Colloidal stability improves lowering strength, indicating processes were strongly affected charge screening double layer...