A5026901817- Cancer Cells and Metastasis
- Digestive system and related health
- Genetic factors in colorectal cancer
- Vitamin D Research Studies
- Colorectal Cancer Surgical Treatments
- Microtubule and mitosis dynamics
- BRCA gene mutations in cancer
- DNA Repair Mechanisms
- 3D Printing in Biomedical Research
- COVID-19 and healthcare impacts
- Colorectal and Anal Carcinomas
- Colorectal Cancer Screening and Detection
- Ferroptosis and cancer prognosis
- Colorectal Cancer Treatments and Studies
- Cancer, Hypoxia, and Metabolism
- Protist diversity and phylogeny
- Wnt/β-catenin signaling in development and cancer
- Body Composition Measurement Techniques
Instituto de Investigaciones Biomédicas Sols-Morreale
2019-2024
Universidad Autónoma de Madrid
2019-2024
Centro de Investigación Biomédica en Red de Cáncer
2019-2023
Hospital Universitario La Paz
2020-2023
Consejo Superior de Investigaciones Científicas
2019-2023
Centre for Biomedical Network Research on Rare Diseases
2019-2022
Hospital de Sant Pau
2022
Hospital La Paz Institute for Health Research
2019
Intestine is a major target of vitamin D and several studies indicate an association between deficiency inflammatory bowel diseases (IBD), but also increased colorectal cancer (CRC) risk mortality. However, the putative effects 1α,25-dihydroxyvitamin D3 (calcitriol), active metabolite, on human colonic stem cells are unknown. Here we show by immunohistochemistry RNAscope in situ hybridization that receptor (VDR) unexpectedly expressed LGR5+ colon tissue normal tumor organoid cultures...
Colon and rectal tumors, often referred to as colorectal cancer, show different gene expression patterns in studies that analyze whole tissue biopsies containing a mix of tumor non-tumor cells. To better characterize colon we investigated the profile organoids generated from endoscopic tumors adjacent normal rectum mucosa therapy-naive cancer patients. We also studied effect vitamin D on these organoid types. Gene profiling was performed by RNA-sequencing. Organoids had shared profoundly...
Plocabulin is a novel microtubule-disrupting antitumor agent of marine origin that currently undergoing phase II clinical trials. has potent antiproliferative and antiangiogenic actions in carcinoma cell lines activity xenografted mice. Here, we used three-dimensional (3D) tumor organoids derived from three colorectal cancer (CRC) patients to study the effect plocabulin personalized assay system ensures dose dependence high reproducibility. The cytotoxicity was an order magnitude higher than...
Abstract Understanding the mechanisms involved in colonic epithelial differentiation is key to unraveling alterations causing inflammatory conditions and cancer. Organoid cultures provide an unique tool address these questions but studies are scarce. We report a system toward enterocytes goblet cells, two major cell lineages, using colon organoids generated from healthy tissue of colorectal cancer patients. Culture medium lacking stemness agents resulted modest ultrastructural phenotype with...
The rising incidence of colorectal cancer (CRC) among young patients is alarming. We aim to characterize the clinico-pathological features and outcomes with early-onset CRC (EOCRC), as well impacts COVID-19 pandemic.We included all pathologically confirmed diagnoses at Hospital Universitario La Paz from October 2016 December 2021. EOCRC cut-off age was 50 years old.A total 1475 diagnosed were included, eighty (5.4%) whom had EOCRC. Significant differences found between later-onset regarding...
BRCA2 is essential for homologous recombination DNA repair. mutations lead to genome instability and increased risk of breast ovarian cancer. Similarly, in BRCA2-interacting proteins are also known modulate sensitivity damage agents established cancer factors. Here we identify the tumor suppressor CDK5RAP3 as a novel helical domain-interacting protein. depletion induced resistance, single-strand annealing upregulation, reduced spontaneous damage-induced genomic instability, suggesting that...
15 Background: Although we seem to be recovering from COVID-19, are now facing the long-term consequence of pandemic on vulnerable subgroup patients such as with early-onset colorectal cancer (EOCRC). We aim study how COVID-19 affected diagnosis and clinic-pathological characteristics, treatment outcomes EOCRC. Methods: This is an observational retrospective including EOCRC diagnosed between September 2016 August 2021 at Hospital Universitario La Paz, Madrid, Spain. Two cohorts were...