A5101689353- CAR-T cell therapy research
- Immune cells in cancer
- Acute Myeloid Leukemia Research
- Immunotherapy and Immune Responses
- Neuroinflammation and Neurodegeneration Mechanisms
- Developmental Biology and Gene Regulation
- Cancer Immunotherapy and Biomarkers
- Protein Degradation and Inhibitors
- Histone Deacetylase Inhibitors Research
- Genomics and Chromatin Dynamics
- Animal Genetics and Reproduction
- interferon and immune responses
- Amyotrophic Lateral Sclerosis Research
- Hippo pathway signaling and YAP/TAZ
- Telomeres, Telomerase, and Senescence
- Melanoma and MAPK Pathways
- Mitochondrial Function and Pathology
- Protease and Inhibitor Mechanisms
- Nerve injury and regeneration
- Epigenetics and DNA Methylation
- Circular RNAs in diseases
- Cancer Mechanisms and Therapy
- Extracellular vesicles in disease
- Renal and related cancers
- Natural Compounds in Disease Treatment
Discovery Institute
2019-2024
Sanford Burnham Prebys Medical Discovery Institute
2019-2024
Soochow University
2023
First Affiliated Hospital of Soochow University
2023
Sichuan Cancer Hospital
2022
University of Electronic Science and Technology of China
2022
Xiangya Hospital Central South University
2020-2022
Central South University
2020-2022
Inner Mongolia Medical University
2020
University College Dublin
2012
Abstract Disrupted antiviral immune responses are associated with severe COVID-19, the disease caused by SAR-CoV-2. Here, we show that 73-amino-acid protein encoded ORF9c of viral genome contains a putative transmembrane domain, interacts membrane proteins in multiple cellular compartments, and impairs processes lung epithelial cell line. Proteomic, interactome, transcriptomic analyses, combined bioinformatic analysis, revealed expression only this highly unstable small impaired interferon...
Variations in many genes linked to sporadic Alzheimer's disease (AD) show abundant expression microglia, but relationships among these remain largely elusive. Here, we establish isogenic human ESC-derived microglia-like cell lines (hMGLs) harboring AD variants CD33, INPP5D, SORL1, and TREM2 loci curate a comprehensive atlas comprising ATAC-seq, ChIP-seq, RNA-seq, proteomics datasets. AD-like signatures are observed mutant SORL1 hMGLs, while integrative multi-omic analysis of combined...
Abstract Acute myeloid leukemia (AML) remains incurable, largely due to its resistance conventional treatments. Here, we find that increased abundance of the ubiquitin ligase RNF5 contributes AML development and survival. High expression in patient specimens correlates with poor prognosis. inhibition decreases cell growth culture, patient-derived xenograft (PDX) samples vivo, delays MLL-AF9–driven leukemogenesis mice, prolonging their causes transcriptional changes overlap those seen upon...
PSGL-1 (P-selectin glycoprotein-1) is a T cell-intrinsic checkpoint regulator of exhaustion with an unknown mechanism action. Here, we show that acts upstream PD-1 and requires co-ligation the cell receptor (TCR) to attenuate activation mouse human CD8+ cells drive terminal exhaustion. directly restrains TCR signaling via Zap70 maintains expression inhibitor Sts-1. deficiency empowers respond low-affinity ligands inhibit growth PD-1-blockade-resistant melanoma by enabling tumor-infiltrating...
Abstract Energy restriction (ER) protects against cerebral ischemic injury, but the underlying mechanism remains largely unclear. Here, rats were fed ad libitum (AL) or on an alternate-day food deprivation intermittent fasting (IF) diet for 3 months, followed by middle artery occlusion (MCAO) surgery. The body weight, infarct volume, and neurological deficit score accessed at designated time points. ELISA, qRT-PCR, Western blotting used to determine cytokine secretion expression of SIRT6,...
Identification of target genes that mediate required functions downstream transcription factors is hampered by the large number whose expression changes when factor removed from a specific tissue and numerous binding sites for in genome. Retinoic acid (RA) regulates via RA receptors bound to response elements (RAREs) which there are thousands vertebrate genomes. Here, we combined chromatin immunoprecipitation sequencing (ChIP-seq) epigenetic marks RNA-seq on trunk wild-type Aldh1a2-/-...
Abstract Background Medulloblastoma (MB) is an aggressive brain tumor that predominantly affects children. Recent high-throughput sequencing studies suggest the noncoding RNA genome, in particular long RNAs (lncRNAs), contributes to MB subgrouping. Here we report identification of a novel lncRNA, lnc-HLX-2-7, as potential molecular marker and therapeutic target Group 3 MBs. Methods Publicly available (RNA-seq) data from 175 patients were interrogated identify lncRNAs differentiate between...
Abstract A key hallmark of cancer, altered metabolism, is central to cancer pathogenesis and therapy resistance. Robust glutamine metabolism among cellular processes regulating tumor progression responsiveness in a number cancers, including melanoma breast cancer. Among mechanisms underlying the increase tumors enhanced uptake mediated by transporters, with SLC1A5 (also known as ASCT2) shown play predominant role. Correspondingly, increased expression coincides poorer survival patients...
Abstract Background We aimed to determine the performance of 18 F-FAPI PET/CT used for preprocedural assessment glioblastoma before radiotherapy. Methods Twelve patients having undergone incomplete surgical resection or biopsy were examined with and MRI scanning All had confirmed tumor residues according findings histopathological and/or long-term clinical radiological follow-ups. Lesion characterization data, including SUV max tumor-to-background ratio (TBR) on attained. compared in terms...
Our objective was to profile genetic pathways whose differential expression correlates with maturation of visual function in zebrafish. Bioinformatic analysis transcriptomic data revealed Jak-Stat signalling as the pathway most enriched eye, develops. Real-time PCR, western blotting, immunohistochemistry and situ hybridization confirm that multiple genes are up-regulated zebrafish eye between 3-5 days post-fertilisation, times associated significant vision. One is proto-oncogene Pim1 kinase,...
Cell therapy is an emerging therapeutic modality with the power to exploit new cancer targets and potentially achieve positive outcomes for patients few other options. Like all synthetic treatments, cell has risk of toxicity via unpredicted off-target behavior. We describe empirical method model off-tumor, reactivity receptors used investigational T therapies. This approach utilizes optimal panel diverse human cell-lines capture large majority protein-coding gene expression in adult tissues....
One approach to understanding how tissue-specific cancers emerge is determine the requirements for "reprograming" such neoplastic cells back their developmentally normal primordial pre-malignant epiblast-like pluripotent state and then scrutinizing spontaneous reconversion a neoplasm, perhaps rendering salient earliest pivotal oncogenic pathway(s) (before other aberrations accumulate in adult tumor). For prototypical malignancy anaplastic thyroid carcinoma (ATC), we found that tonic RAS...
Acute leukemia is a particularly problematic collection of hematological cancers, and, while somewhat rare, the survival rate patients typically abysmal without bone marrow transplantation. Furthermore, traditional chemotherapies used as standard-of-care for cause significant side effects. Understanding evolution to identify novel targets therefore, drug treatment regimens medical need. Genomic rearrangements and other structural variations (SVs) have long been known be causative pathogenic...
Abstract Identification of target genes that mediate required functions downstream transcription factors is hampered by the large number whose expression changes when factor removed from a specific tissue and numerous binding sites for in genome. Retinoic acid (RA) regulates via RA receptors bound to response elements (RAREs) which there are thousands vertebrate genomes. Here, we combined ChIP-seq epigenetic marks RNA-seq on trunk wild-type Aldh1a2 -/-embryos lacking synthesis exhibit body...
ABSTRACT Acute myeloid leukemia (AML) remains incurable, largely due to its resistance conventional treatments. Here, we found that increased expression and abundance of the ubiquitin ligase RNF5 contributes AML development survival. High in patients correlated with poor prognosis. inhibition decreased cell growth culture vivo , blocked MLL-AF9–driven leukemogenesis mice, prolonging their led transcriptional changes overlapped those seen upon HDAC1 inhibition. induced formation K29 chains on...
Summary/Abstract We previously identified the adhesion molecule PSGL-1 as a T cell intrinsic immune checkpoint regulator of exhaustion. Here we show that ability to restrain TCR sginaling correlates with decreased expression Zap70 inhibitor Ubash3b (Sts-1) in PSGL-1-deficient cells. PSGL-1-deficency cells supports antitumor responses PD-1 blockade resistant melanoma wherein tumor-specific CD8 + sustain an enhanced metabolic state, elevated gene signature promotes increased glycolysis and...