A5011505678- Malaria Research and Control
- Mosquito-borne diseases and control
- vaccines and immunoinformatics approaches
- Complement system in diseases
- Hemoglobinopathies and Related Disorders
- Vector-borne infectious diseases
- Erythrocyte Function and Pathophysiology
- Iron Metabolism and Disorders
- Neonatal Health and Biochemistry
- Computational Drug Discovery Methods
- Advanced Proteomics Techniques and Applications
- Trace Elements in Health
- Aquaculture disease management and microbiota
- Antimicrobial Peptides and Activities
- Parasitic Infections and Diagnostics
- Hemoglobin structure and function
- Pharmaceutical studies and practices
- Monoclonal and Polyclonal Antibodies Research
- Diverse Scientific Research Studies
- HIV Research and Treatment
- Hepatitis Viruses Studies and Epidemiology
- Invertebrate Immune Response Mechanisms
- RNA and protein synthesis mechanisms
- Biosensors and Analytical Detection
- Viral Infections and Vectors
Universidad Rey Juan Carlos
2022-2025
Research Institute Hospital 12 de Octubre
2012-2025
Universidad Complutense de Madrid
2012-2020
Significance Malaria remains one of the main health threats in developing world, with staggering social and economic costs. Resistance to artemisins, pharmacological tool currently available against malaria, has been widely reported. Borrelidin, a natural compound that inhibits threonyl-tRNA synthetase, long studied for its antibacterial antiparasitic properties, but undesirable toxic effects prevented further clinical development. Here we present group borrelidin derivatives retain their...
Babesiosis is an emerging zoonosis now found in several areas of the world. Using PCR and indirect immunofluorescence assay, we have diagnosed first case human babesiosis caused by Babesia microti Spain. Diagnosis was delayed because nonspecific clinical symptoms that occurred immunocompetent patient.
Malaria has exerted potent selective pressure on the human genome over millennia and been a significant force in shaping evolution. We have determined 424 individuals living malaria-hyperendemic areas Ghana Democratic Republic of Congo, genotypes at loci PIEZO1, G6PD, HBB, PKLR. By qPCR we also estimated P. falciparum parasitemia all these subjects. found that 41% tested had one protective variant, 20.5% two variants, 6.4% three 0.7% four variants. confirmed Pz_E756del, G6pd A-, HbS are...
One of the main challenges in compiling complete collection protein antigens from pathogens for selection vaccine candidates or intervention targets is to acquire a broad enough representation them be recognized by highly diversified immunoglobulin repertoire human populations. Dried serum spot sampling (DSS) retains large circulating immunoglobulins each individual that can representative population, according sample size. In this work, shotgun proteomics an infectious pathogen based on DSS...
Iron overload caused by hereditary hemochromatosis (HH) increases free reactive oxygen species that, in turn, induce lipid peroxidation. Its 4-hydroxynonenal (HNE) by-product is a well-established marker of peroxidation since it reacts with accessible proteins deleterious consequences. Indeed, elevated levels HNE are often detected wide variety human diseases related to oxidative stress. Here, we evaluated HNE-modified the membrane erythrocytes from HH patients and organs Hfe-/- male female...
Malaria infection in humans elicits a wide range of immune responses that can be detected peripheral blood, but we lack detailed long-term follow-up data on the primary and subsequent infections lead to naturally acquired immunity. Studies antimalarial mice have been based models yielding homogenous profiles. Here, present mouse model which heterogeneous course Plasmodium yoelii lethal malaria is produced non-congenic ICR strain allow comparison among different immunological clinical...
Assessment of serological Plasmodium falciparum –specific antibodies in highly endemic areas provides valuable information about malaria status and parasite exposure the population. Although evidence is commonly determined by -specific immunoglobulin G (IgG) levels; IgM IgA are likely markers that remain relatively unexplored. Previous studies on responses have been based their affinity for single antigens with shortage immune analysis against whole proteome. Here, we provide how P....
Abstract Incomplete non-sterile immunity to malaria is attained in endemic regions after recurrent infections by a large percentage of the adult population, who carry parasite asymptomatically. Although blood-stage Plasmodium falciparum rapidly elicits IgG responses, target antigens partially protective and non-protective antibodies as well basis for acquisition these remain largely unknown. We performed IgG-immunomics screen P. identify epitopes associated with exposure clinical disease....
Vaccines blocking malaria parasites in the blood-stage diminish mortality and morbidity caused by disease. Here, we isolated antigens from total parasite proteins antibody affinity chromatography to test an immunization against lethal infection a murine model. We used sera of self-resistant ICR mice Plasmodium yoelii 17XL for purification their IgGs which were subsequently employed isolate that inoculated immunize BALB/c mice. The presence specific antibodies vaccinated serum was studied...
Abstract Incomplete non-sterile immunity to malaria is attained in endemic regions after recurrent infections by a large percentage of the adult population, who carry parasite asymptomatically. Although blood-stage Plasmodium falciparum rapidly elicits IgG responses, target antigens partially protective and non-protective antibodies as well basis for acquisition these remain largely unknown. We performed IgG-immunomics screen P. identify epitopes associated with exposure clinical disease....