A5051034667- vaccines and immunoinformatics approaches
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- HIV Research and Treatment
- Biochemical and Molecular Research
- Peptidase Inhibition and Analysis
- Allergic Rhinitis and Sensitization
- Cytomegalovirus and herpesvirus research
- Enzyme Structure and Function
- Dermatology and Skin Diseases
- Ubiquitin and proteasome pathways
- Influenza Virus Research Studies
- Biochemical Acid Research Studies
- Autoimmune Bullous Skin Diseases
- Glycosylation and Glycoproteins Research
- Machine Learning in Bioinformatics
- COVID-19 Clinical Research Studies
- Trypanosoma species research and implications
- Poxvirus research and outbreaks
- Urticaria and Related Conditions
- Toxin Mechanisms and Immunotoxins
- Vaccine Coverage and Hesitancy
Universidad Complutense de Madrid
2016-2025
Research Institute Hospital 12 de Octubre
2022
University of Catania
2017-2021
Hospital Universitario Ramón y Cajal
2020
University College London
2020
Aston University
2018
Dana-Farber Cancer Institute
2001-2007
Harvard University
2001-2007
Boston University
2005
Palo Alto Institute
2002
Abstract The sequence of a novel hemopoietic cytokine was discovered in computational screen genomic databases, and its homology to mouse thymic stromal lymphopoietin (TSLP) suggests that it is the human orthologue. Human TSLP proposed signal through heterodimeric receptor complex consists new member hemopoietin family termed IL-7R α-chain. Cells transfected with both subunits proliferated response purified, recombinant TSLP, induced phosphorylation Stat3 Stat5. TSLPR IL-7Rα are principally...
Abstract Background ADP-ribosylation is an enzyme-catalyzed posttranslational protein modification in which mono(ADP-ribosyl)transferases (mARTs) and poly(ADP-ribosyl)transferases (pARTs) transfer the ADP-ribose moiety from NAD onto specific amino acid side chains and/or units on target proteins. Results Using a combination of database search tools we identified genes encoding recognizable pART domains public genome databases. In humans, family encompasses 17 members. For 16 these genes,...
We have developed PVS (Protein Variability Server), a web-based tool that uses several variability metrics to compute the absolute site in multiple protein-sequence alignments (MSAs). The is then assigned user-selected reference sequence consisting of either first alignment or consensus sequence. Subsequently, performs tasks are relevant for structure-function studies, such as plotting and visualizing 3D-structure. Neatly, also implements some other thought facilitate design epitope...
The lack of available vaccines against African swine fever virus (ASFV) means that the evaluation new immunization strategies is required. Here we show fusion extracellular domain ASFV Hemagglutinin (sHA) to p54 and p30, two immunodominant structural viral antigens, exponentially improved both humoral cellular responses induced in pigs after DNA immunization. However, with resulting plasmid (pCMV-sHAPQ) did not confer protection lethal challenge virulent E75 ASFV-strain. Due fact CD8(+)...
The recently emerged SARS-CoV-2 causing the ongoing COVID-19 pandemic is particularly virulent in elderly while children are largely spared. Here, we explored potential role of cross-reactive immunity acquired from pediatric vaccinations and exposure to common human pathogens protection pathology COVID-19. To that end, sought for peptide matches (identity ≥ 80%, at least 8 residues) proteomes 25 vaccine antigens, subsequently predicted their T B cell reactivity identify epitopes. We found...
Allergen immunotherapy (AIT) is the only curative treatment for allergy. AIT faces pitfalls related to efficacy, security, duration, and patient compliance. Novel vaccines overcoming such inconveniences are in demand.We sought study immunologic mechanisms of action novel targeting dendritic cells (DCs) generated by coupling glutaraldehyde-polymerized grass pollen allergoids nonoxidized mannan (PM) compared with (P) or native extracts (N).Skin prick tests basophil activation N, P, PM were...
Abstract ADP‐ribosyltransferases including toxins secreted by Vibrio cholera, Pseudomonas aerurginosa , and other pathogenic bacteria inactivate the function of human target proteins attaching ADP‐ribose onto a critical amino acid residue. Cross‐species polymerase chain reaction (PCR) database mining identified orthologs these ADP‐ribosylating in humans mouse. The genome contains four functional toxin‐related ADP‐ribosyltransferase genes ( ARTs ) two related intron‐containing pseudogenes;...
DNA methylation plays a key role in the regulation of gene expression. The most common type modification consists cytosine CpG dinucleotide. At present time, there is no method available for prediction sites. Therefore, this study we have developed support vector machine (SVM)-based dinucleotides. Initially SVM module was from human data human-specific This achieved MCC and AUC 0.501 0.814, respectively, when evaluated using 5-fold cross-validation. performance SVM-based better than...
The EGF-like domain of smallpox growth factor (SPGF) targets human ErbB-1, inducing tyrosine phosphorylation certain host cellular substrates via activation the receptor’s kinase and thereby facilitating viral replication. Given these findings, low molecular weight organic inhibitors ErbB-1 kinases might function as antiviral agents against smallpox. Here we show that CI-1033 related 4-anilinoquinazolines inhibit SPGF-induced DNA synthesis, protein activation, c-Cbl association with...
Trypanosoma cruzi infection causes Chagas disease which affects 7 million people worldwide. Two drugs are available to treat it: benznidazole and nifurtimox. Although both efficacious against the acute stage of disease, this is usually asymptomatic goes undiagnosed untreated. Diagnosis achieved at chronic stage, when life-threatening heart and/or gut tissue disruptions occur in ~30% those chronically infected. By then drugs´ efficacy reduced, but not their associated high toxicity. Given...
The EGF-like domain of smallpox growth factor (SPGF) targets human ErbB-1, inducing tyrosine phosphorylation certain host cellular substrates via activation the receptor’s kinase and thereby facilitating viral replication. Given these findings, low molecular weight organic inhibitors ErbB-1 kinases might function as antiviral agents against smallpox. Here we show that CI-1033 related 4-anilinoquinazolines inhibit SPGF-induced DNA synthesis, protein activation, c-Cbl association with...
Prediction of peptide binding to major histocompatibility complex (MHC) molecules is a basis for anticipating T-cell epitopes, as well epitope discovery-driven vaccine development. In the human, MHC are known human leukocyte antigens (HLAs) and extremely polymorphic. HLA polymorphism differential binding, until now limiting practical use current epitope-prediction tools Here, we describe web server, PEPVAC (Promiscuous EPitope-based VACcine), optimized formulation multi-epitope vaccines with...