A5013709830- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- HIV Research and Treatment
- vaccines and immunoinformatics approaches
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- Immunodeficiency and Autoimmune Disorders
- Immune Response and Inflammation
- Cancer Immunotherapy and Biomarkers
- HIV/AIDS drug development and treatment
- Bacteriophages and microbial interactions
- Influenza Virus Research Studies
- Peptidase Inhibition and Analysis
- Transgenic Plants and Applications
- RNA and protein synthesis mechanisms
- Force Microscopy Techniques and Applications
- RNA Interference and Gene Delivery
- Hematopoietic Stem Cell Transplantation
- Cytomegalovirus and herpesvirus research
- Toxin Mechanisms and Immunotoxins
- Diabetes and associated disorders
- Virus-based gene therapy research
Dana-Farber Cancer Institute
2016-2025
Harvard University
2016-2025
University of Massachusetts Boston
2023
Boston University
1983-2019
Vanderbilt University
1981-2019
Institute of Materials Research and Engineering
2019
Singapore-MIT Alliance for Research and Technology
2019
Massachusetts Institute of Technology
2019
Harvard University Press
1985-2014
Northeastern University
2014
A series of monoclonal antibodies was used to define three discrete stages human intrathymic T-cell differentiation. The earliest stage confined <10% thymocytes, which were·reactive with both OKT9 and OKT10. Subsequently, approximately 70% thymocytes acquired a thymocyte-restricted antigen, OKT6, lost expressed reactivity OKT4 OKT5. These last two were previously shown inducer (helper) cytotoxic/suppressor populations, respectively, in peripheral blood. + , OKT5 OKT6 “common” thymocyte...
Three novel monoclonal antibodies (designated OKT1, OKT3, and OKT4) were generated against surface determinants of human peripheral T cells. Both OKT1 OKT3 reacted with all cells 5 to 10 percent thymocytes but differed in their reactivities cell lines. By contrast, OKT4 55 80 thymocytes. All three selective for the blood that they did not react normal B cells, null monocytes, or granulocytes.
A monoclonal antibody was produced to human peripheral blood T cells. This hybridoma antibody, termed OKT4, reactive by indirect immunofluorescence with only 55-60% of the cell population (OKT4+) and unreactive normal B cells, null macrophages. The OKT4- contained previously described TH2+ subset that has been shown contain cytotoxic/suppressor With cell-sorter separation OKT4+ it these subsets were functionally discrete. Both gave proliferative responses concanavalin A, alloantigens,...
Abstract A monoclonal antibody directed at a determinant on human peripheral blood monocytes was produced and characterized. This hybridoma antibody, termed OKM1, reactive by indirect immunofluorescence complement- (C) mediated lysis with adherent mononuclear cells. OKM1 unreactive lymphocytes, thymocytes, lymphoblastoid cell lines, tumor cells of the T or B lineage. In contrast, acute myelomonocytic leukemia granulocytes were antibody. Pretreatment C before culture soluble antigens totally...
Monoclonal antibodies were produced against a human cytotoxic T cell clone, CT8III (specificity: HLA-A3), with the view of defining clonally restricted (clonotypic) surface molecules involved in its antigen recognition function. Two individual antibodies, termed anti-Ti1A and anti-Ti1B, reacted exclusively clone when tested on panel 80 additional clones from same donor, resting or activated cells, B macrophages, thymocytes, other hematopoietic cells. More importantly, two inhibited...
Evidence is presented that the OKTA+ T cell subset in man, defined by a monoclonal hybridoma antibody, provides help for B lymphocyte differentiation PWM driven system. Both proliferation and intracytoplasmic immunoglobulin synthesis are facilitated OKT4+ not OKT4- cells. Given earlier studies demonstrating cells were necessary generation of cytotoxic present study OKT+ cells, it would appear population major human helper (inducer) subset.
The nature of Ia antigens which appear on human T cells after activation and the stimuli required for their expression was examined utilizing a monoclonal antibody reactive with antigen framework. were purified using antibodies directed either at entire T-cell population (OKT3) or inducer subset (OKT4). By indirect immunofluorescence, it shown that contains no detectable Ia+ in resting state. In contrast, excess 60% expresses alloactivation mixed lymphocyte culture. Moreover, these are...
Abstract A monoclonal antibody directed at a determinant on human peripheral blood T cells was produced and characterized. This hybridoma antibody, termed OKT1, reactive by indirect immunofluorescence with the entire cell population subset of thymocytes. In contrast, OKT1 unreactive normal B cells, Null macrophages, ≥90% These findings suggested that defines mature differentiation antigen. support this notion observation acute lymphoblastic leukemia were nonreactive whereas chronic...
To determine whether abnormalities of immunoregulatory T cells are associated with multiple sclerosis (MS), we characterized peripheral lymphocytes in 33 patients untreated MS and compared them 42 normal persons 29 age-matched control subjects who had other neurologic diseases. For this analysis, used monoclonal antibodies to the surface antigens helper (T4) suppressor (T5) T-cell subsets a common antigen (T3). In contract controls diseases, reduced percentage T3-positive (T3+) (P less than...
Recent studies suggested that the clonally unique Ti epitopes defined by non-cross-reactive monoclonal antibodies might represent variable regions of antigen receptor. Here we determine whether such anti-Ti could trigger clonal T cell activation. Anticlonotypic to 49/43-kdalton heterodimer a given clone or 20/25-kdalton membrane associated monomorphic T3 molecule selectively induce proliferation and IL-2 secretion when linked solid support. In contrast, anti-T4 anti-T8 under same conditions...
Human T cell subpopulations have been defined on the basis of differential expression either Fc receptors or specific cell-surface antigens. In this study, we utilized a series monoclonal antibodies reactive with cells, monocytes, and Ia antigens to characterize isolated cells bearing for portion IgG (T gamma) IgM mu. The results showed that mu population contained both inducer (OKT4+) cytotoxic/suppressor (OKT5+) populations was similar unfractionated population, whereas gamma subset few...
The distribution of T cells and cell subsets was examined within the human central nervous system in active lesions from seven patients with chronic multiple sclerosis. monoclonal antibodies anti-T11, anti-T4, anti-T8 were used to detect total (whole) cells, helper suppressor-cytotoxic respectively, a antibody against Ia for macrophages B cells. Lesion progression associated large numbers T4+ at lesion margin these extended great distances into adjacent normal-appearing white matter. T8+...
Introduction of TCRα transgene, TCRβ or both into RAG-2 -/- mice differentially rescues T cell development. have small numbers TCR - CD4 CD8 (double negative, DN) thymocytes that express CD3γδε and ζ proteins intracellularly. a but not the background restored normal thymocytes. These cells were + positive, DP) expressed amounts surface chain dimers in association with ζ. α β transgenes developed DP single positive Thus, subunit, possibly novel CD3 complex, participates DN to transition.
The crystal structure of a complex involving the D10 T cell receptor (TCR), 16-residue foreign peptide antigen, and I-Ak self major histocompatibility (MHC) class II molecule is reported at 3.2 angstrom resolution. TCR oriented in an orthogonal mode relative to its peptide-MHC (pMHC) ligand, necessitated by amino-terminal extension residues projecting from MHC antigen-binding groove as part mini beta sheet. Consequently, disposition complementarity-determining region loops altered that most...
Abstract Viral infections are often associated with immunodeficiency states. Although T lymphocytes have been thought to suppress the host's response, precise etiology remains unclear. Therefore, we characterized from six patients during both acute and convalescent phases of infectious mononucleosis (IM) monoclonal antibodies (titer, 10(-5) 10(-7) antigens restricted TH2- helper (T4) TH2 suppressor (T5) cell subsets as well a common antigen (T3) HLA-D related Ia antigens. It was found that...