A5047033032- Alzheimer's disease research and treatments
- Autophagy in Disease and Therapy
- Parkinson's Disease Mechanisms and Treatments
- Metabolism, Diabetes, and Cancer
- Endoplasmic Reticulum Stress and Disease
- Tea Polyphenols and Effects
- Polyamine Metabolism and Applications
- Amino Acid Enzymes and Metabolism
- Neuroscience and Neuropharmacology Research
- Cancer-related Molecular Pathways
- Pancreatic function and diabetes
- Neurogenesis and neuroplasticity mechanisms
- Phytochemicals and Antioxidant Activities
- Sphingolipid Metabolism and Signaling
- Lysosomal Storage Disorders Research
- Adipose Tissue and Metabolism
- Diet, Metabolism, and Disease
- Mitochondrial Function and Pathology
- Cannabis and Cannabinoid Research
- Tryptophan and brain disorders
- Diabetes Treatment and Management
- Diet and metabolism studies
- Immune Cell Function and Interaction
- Genomics, phytochemicals, and oxidative stress
- Medicinal Plants and Neuroprotection
Chung Shan Medical University
2016-2025
Chung Shan Medical University Hospital
2015-2025
JSS Academy of Higher Education and Research
2022
Stony Brook University
2015-2020
State University of New York
2017-2020
National Taiwan University of Sport
2016
Chang Gung Memorial Hospital
2010
National Taiwan University
1998-2008
Institute of Molecular Biology
2008
Taipei Medical University
2001
An isocratic HPLC procedure was developed for simultaneous determination of six catechins, gallic acid, and three methylxanthines in tea water extract. A baseline separation achieved on a Cosmosil C18-MS packed column with solvent mixture methanol/doubly distilled water/formic acid (19.5:80.2:0.3, v/v/v) as mobile phase. gradient also provided the these components. The contents have been measured infusions range green tea, oolong pu-erh products sold consumed China, Japan, Taiwan. When 15...
Black tea is one of the world's most popular beverages, and its health-promoting effects have been intensively investigated. The antiobesity hypolipidemic black attracted increasing interest, but mechanisms underlying these phenomena remain unclear. In present study, major component theaflavins were assessed for their hepatic lipid-lowering potential when administered in fatty acid overload conditions both cell culture an animal experimental model. We found that significantly reduced lipid...
Dabsyl chloride (dimethylaminoazobenzene sulfonyl chloride), a useful chromophoric labeling reagent for amino acids and amines, was developed in this laboratory 1975. Although several methods have been to determine various types of acids, quick easy method determining theanine, GABA, other has not one HPLC system. In paper are analyzed the free acid contents theanine GABA different teas (green tea, black oolong Pu-erh tea) with dabsylation reverse phase high-performance liquid chromatography...
It is now clear that insulin signaling has important roles in regulation of neuronal functions the brain. Dysregulation brain been linked to neurodegenerative disease, particularly Alzheimer's disease (AD). In this regard, there evidence improvement neuroprotective activity against amyloid β (Aβ)-induced neurotoxicity for patients with AD. Linagliptin an inhibitor dipeptidylpeptidase-4 (DPP-4), which improves impaired secretion and downstream peripheral tissues. However, whether protective...
Abstract Insulin resistance is the primary characteristic of type 2 diabetes which as a result insulin signaling defects. It has been suggested that tea polyphenol (–)‐epigallocatechin‐3‐gallate (EGCG) displays some antidiabetic effects, but mechanism for EGCG insulin‐enhancing effects incompletely understood. In present study, investigations on are performed in insulin‐responsive human HepG2 cells cotreated with high glucose. We found glucose condition causes significant increasing Ser307...
Abstract (−)‐Epigallocatechin‐3‐gallate (EGCG), a major polyphenol in green tea, was shown to have cancer chemopreventive activity. In this study, we examined the antimetastatic effects of EGCG or combination and dacarbazine on B16‐F3m melanoma cells vitro vivo. First, potentials five tea catechins were by soft agar colony formation assay, results show that more effective than other inhibiting formation. Second, dose‐dependently inhibited cell migration invasion Transwell assay. Third,...
Abstract p53 mediates the DNA damage response (DDR) by regulating expression of genes implicated in cell cycle arrest, senescence, programmed death (PCD), and metabolism. Herein we demonstrate that human alkaline ceramidase 2 (ACER2) is a novel transcriptional target its transactivation DDR. We found overexpression or activation ionizing radiation (IR) upregulated ACER2 cells. Two putative responsive elements (p53REs) were first intron gene, Chromatin Immunoprecipitation (ChIP) assays...
The cancer-preventive activities of quercetin have been extensively studied in invasive breast cancer; however, the role on triple-negative cancer (TNBC) with overexpression insulin-like growth factor-1 receptor (IGF1R) has not resolved. In this article, we demonstrated that inhibited activation IGF1R and its downstream kinases Akt Erk1/2 a dose-dependent manner human MDA-MB-231 cells (TNBC cell line). Related to this, markedly suppressed metastatic phenotype epithelial–mesenchymal...
The F-box protein S-phase kinase-associated 2 (Skp2), which acts as an oncogene through targeting p27 for degradation, is overexpressed in many different human cancers. Skp2 can play important role breast cancer progression and may also be a novel molecular target the treatment of cancer, especially estrogen receptor (ER)/human epidermal growth factor (HER2) negative Unfortunately, specific drugs that are unavailable at present. Therefore, it to explore whether commonly used chemopreventive...
Dyshomeostasis of both ceramides and sphingosine-1-phosphate (S1P) in the brain has been implicated aging-associated neurodegenerative disorders humans. However, mechanisms that maintain homeostasis these bioactive sphingolipids remain unclear. Mouse alkaline ceramidase 3 (Acer3), which preferentially catalyzes hydrolysis C18:1-ceramide, a major unsaturated long-chain ceramide species brain, is upregulated with age mouse brain. Acer3 knockout causes an age-dependent accumulation various...
Abelmoschus esculentus (AE), a commonly consumed vegetable, is well-known for its anti-hyperglycemic effects. However, few scientific reports have identified targets because mucilage increases the difficulty of manipulation. We recently reported extraction steps to obtain subfractions AE, which were found attenuate adverse effects high glucose and fatty acid in vitro. In this study, we used modified type 2 diabetic rats explore whether AE can improve metabolic disturbances caused by insulin...
Whether hispidulin, a flavone from traditional Chinese medicine, can modulate the anticancer effects of tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL), cytokine currently in clinical trials was investigated. In present study, we found that hispidulin potentiated TRAIL-induced apoptosis human ovarian cancer cells and converted TRAIL-resistant to TRAIL-sensitive cells. When examined for its mechanism, highly effective activation caspases 8 caspase 3 consequent...
Background Depression is highly associated with dementia, and this study will compare the frequencies, severity, symptoms of depression between dementia Lewy bodies (DLB) Alzheimer's disease (AD). Methods Frequency was determined according to DSM-IV criteria for major or National Institute Mental Health in AD (NIMH-dAD). Severity were assessed using Hamilton Rating Scale, Cornell Scale Dementia, subscale Neuropsychiatric Inventory. The rates depressive compared DLB. Results A total 312...
Abelmoschus esculentus (AE) has been used in traditional medicine to ameliorate hyperglycemia, but its mucilage increased bioassay difficulties. We have obtained a series of AE subfractions. Among them F1 and F2 regulated dipeptidyl peptidase-4 (DPP-4) type 1 glucagon-like peptide receptor (GLP-1R), the treatment targets for 2 diabetes. F1, fraction residues (FR) showed advantage on different aspects, which attenuates insulin resistance metabolic disorder vivo, prevents renal-tubular change...
Deficiency of insulin signaling has been linked to diabetes and ageing-related neurodegenerative diseases such as Alzheimer's disease (AD). In this regard, brains exhibit defective receptor substrate-1 (IRS-1) hence result in alteration progression AD, the most common cause dementia. Consequently, dysregulation plays an important role amyloid-β (Aβ)-induced neurotoxicity. As derivation induced pluripotent stem cells (iPSC) involves cell reprogramming, it may provide a means for regaining...
3,5,3′,4′,5′-Pentamethoxystilbene (MR-5) is a synthetically methoxylated analogue of resveratrol and has been suggested to have antitumor activity because structural similarity resveratrol. Herein, we investigate the antiproliferative effect MR-5 in human breast cancer MCF-7 cells demonstrate that had more potent inhibition on cell growth compared with other derivatives. Exploring growth-inhibitory mechanisms MR-5, found it accompanied by G1 cycle arrest, which coincides marked regulatory...
Loss of p27Kip1 is associated with a poor prognosis in breast cancer. According to previous findings, decrease levels mainly the result enhanced proteasome-dependent degradation mediated by its specific ubiquitin ligase subunit S-phase kinase protein 2 (Skp2). Epigallocatechin-3-gallate (EGCG), main constituent green tea, was found stabilize cancer, but whether this effect through changes Skp2 expression remains unclear. Here we investigated mechanisms involved EGCG’s growth inhibition...
Nucleophosmin (NPM) is an important phosphoprotein with pleiotropic functions in various cellular processes. Although phosphorylation has been postulated as functional determinant, possible regulatory roles of this modification on NPM are not fully characterized. Here, we find that dephosphorylated threonine residues (Thr199 and Thr234/237) response to UV-induced DNA damage. Further experiments indicate the serine/threonine protein phosphatase PP1β a physiological under both genotoxic stress...
Peptidylarginine deiminase type 2 (PADI2) deiminates (or citrullinates) arginine residues in protein to citrulline a Ca 2+ -dependent manner, and is found lymphocytes macrophages.Vimentin an intermediate filament well-known substrate of PADI2.Citrullinated vimentin ionomycin-induced macrophage apoptosis.Citrullinated the target anti-Sa antibodies, which are specific rheumatoid arthritis, play critical role pathogenesis disease.To investigate PADI2 apoptosis, we generated Jurkat cell line...