A5038535929- Influenza Virus Research Studies
- Respiratory viral infections research
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- RNA Research and Splicing
- RNA regulation and disease
- RNA and protein synthesis mechanisms
- Animal Disease Management and Epidemiology
- Cancer-related gene regulation
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Lung Cancer Treatments and Mutations
- Circular RNAs in diseases
- Immune Cell Function and Interaction
- Cancer-related Molecular Pathways
- SARS-CoV-2 and COVID-19 Research
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Immune Response and Inflammation
- Ubiquitin and proteasome pathways
- interferon and immune responses
- Cancer Immunotherapy and Biomarkers
- Cancer Cells and Metastasis
- Mosquito-borne diseases and control
National Yang Ming Chiao Tung University
2004-2025
Chang Gung Memorial Hospital
2010-2025
Chang Gung University
2016-2025
Linkou Chang Gung Memorial Hospital
2020-2025
Johns Hopkins University
2017-2024
Tianjin Medical University General Hospital
2023
Bloomberg (United States)
2022
University of Oxford
2018
Institute of Biomedical Sciences, Academia Sinica
2014
National Taiwan University
2005-2012
Oral squamous cell carcinoma (OSCC) is a highly aggressive cancer and the fourth leading malignancy among males in Taiwan. Some pathogenic bacteria are associated with periodontitis oral cancer. However, comprehensive profile of microbiome during cancer's progression from early stage to late still unclear. We profiled microbiota identified biomarkers OSCC. The an rinse 51 healthy individuals 197 OSCC patients at different stages were investigated using 16S rRNA V3V4 amplicon sequencing,...
Preclinical studies have shown that asparagine deprivation enhances T cell antitumour responses. Here we apply compassionate use of L-asparaginase, usually employed to treat blood malignancies, on patients with recurrent metastatic nasopharyngeal carcinoma. The L-asparaginase notably immune-checkpoint blockade therapy in by strengthening CD8+T fitness. Our study shows this combination is a promising avenue for clinical application and provides further mechanistic insight into how restriction...
Mcl-1 is one Bcl-2 family member that plays a pivotal role in animal development. The extremely labile nature of the protein itself and fact level critical determinant various cell survival pathways suggest cellular processes regulate stability are as important those synthesis. Although transcriptional stimulation synthesis response to stimuli has been well documented, regulation hardly explored. In this study, we identified translationally controlled tumor (TCTP) was factor interacted with...
Translationally controlled Tumor Protein (TCTP) is an evolutionally highly conserved protein which has been implicated in many cellular functions that are related to cell growth, death, and even the allergic response of host. To address physiological roles TCTP, we generated TCTP knockout mice by targeted gene disruption. Heterozygous mutants appeared be developmentally normal. However, homozygous (TCTP(-/-)) were embryonic lethal. TCTP(-/-) embryos smaller size than control littermates at...
Oral squamous cell carcinoma is a prominent cancer worldwide, particularly in Taiwan. By integrating omics analyses 50 matched samples, we uncover Taiwanese patients predominant mutation signature associated with cytidine deaminase APOBEC, which correlates the upregulation of APOBEC3A expression APOBEC3 gene cluster at 22q13. significantly higher tumors carrying APOBEC3B-deletion allele(s). High-level better overall survival, especially among alleles, as examined second cohort (n = 188; p...
During neoplastic development, a multitude of changes in genome-encoded information are progressively selected to confer growth and survival advantages tumor cells. microRNAs-mRNAs regulatory networks, given their role as critical layer robust gene expression control, frequently altered neoplasm. However, whether how these perturbations impact metabolic homeostasis remains largely unresolved. Methods: Through targeted miRNA screening, we uncovered an oral squamous cell carcinoma...
Colorectal cancer (CRC) is one of the most common cancers and causes cancer-related death worldwide. In patients with CRC, metastasis a crucial problem that leads to treatment failure primary cause lethality colon cancer. Long noncoding RNAs (lncRNAs) have recently emerged as critical molecules in development, cell growth, apoptosis, CRC.We investigated transcriptome profiles human lncRNAs tumor tissues corresponding normal mucosa two CRC by using microarray approach. The expression levels...
Despite their lack of protein-coding potential, long noncoding RNAs (lncRNAs) and circular (circRNAs) have emerged as key determinants in gene regulation, acting to fine-tune transcriptional signaling output. These RNA transcripts are known affect expression messenger (mRNAs) via epigenetic post-transcriptional regulation. Given widespread target spectrum, well extensive modes action, a complete understanding biological relevance will depend on integrative analyses systems data at various...
Abstract TP53 mutations are recognized to correlate with a worse prognosis in individuals non-small cell lung cancer (NSCLC). There exists an immediate necessity pinpoint selective treatment for patients carrying mutations. Potential drugs were identified by comparing drug sensitivity differences, represented the half-maximal inhibitory concentration (IC50), between mutant and wild-type NSCLC lines using database analysis. In addition, clinical data from collected evaluate both their status...
// Shu-Jen Chen 1, 2, 3, * , Hsuan Liu Chun-Ta Liao 4, Po-Jung Huang 5 Yi 2 An Hsu 3 Petrus Tang Yu-Sun Chang Hua-Chien Tzu-Chen Yen 6 1 Department of Biomedical Sciences, Gung University, Taoyuan, 33302, Taiwan Genomic Core Laboratory, Graduate Institute 4 Otorhinolaryngology, Memorial Hospital, 33305, Bioinformatics Nuclear Medicine, These authors have contributed equally to this work Correspondence to: Yen, e-mail: yen1110@adm.cgmh.org.tw Chen, hcchen@actgenomics.com Keywords:...
Adenosine-to-inosine RNA editing constitutes a crucial component of the cellular transcriptome and critically underpins organism survival development. While recent high-throughput approaches have provided comprehensive documentation editome, its functional output remains mostly unresolved, particularly for events in non-coding regions. Gene ontology analysis known targets unveiled preponderance genes related to apoptosis regulation, among which proto-oncogenes XIAP MDM2 encode two most...
The primary role of cytoplasmic viral RNA-dependent RNA polymerase (RdRp) is genome replication in the cellular cytoplasm. However, picornaviral RdRp denoted 3D (3Dpol) also enters host nucleus, where its function remains unclear. In this study, we describe a novel mechanism attack which 3Dpol nucleus through nuclear localization signal (NLS) and targets pre-mRNA processing factor 8 (Prp8) to block splicing mRNA synthesis. fingers domain associates with C-terminal region Prp8, contains...
Radiotherapy is an indispensable treatment modality in head and neck cancer (HNC), while radioresistance the major cause of failure. The aim this study to identify a prognostic molecular signature associated with radio-resistance HNC for further clinical applications. Affymetrix cDNA microarrays were used globally survey different transcriptomes between cell lines isogenic radioresistant sublines. KEGG Partek bioinformatic analytical methods assess functional pathways radioresistance....
Inactivated influenza vaccines induce greater antibody responses in females than males among both humans and mice. To test the breadth of protection, we used recombinant mouse-adapted A/California/2009 (maA/Cal/09) H1N1 viruses containing mutations at one (1M), two (2M), or three (3M) antigenic sites, addition to a virus 1M mutation substitution Ca2 site (Sub) with derived from an H5 hemagglutinin (HA) challenge mice sexes. Following maA/Cal/09 vaccination, produced virus-specific,...
The centromere is a highly specialized chromosomal element that essential for chromosome segregation during mitosis. Centromere integrity must therefore be properly preserved and strictly dependent upon the establishment maintenance of surrounding chromatin structure. Here we identify WDHD1, WD40-domain HMG-domain containing protein, as key regulator function. We show WDHD1 associates with centromeres in cell cycle-dependent manner, coinciding mid-to-late S phase. down-regulation compromises...
ABSTRACT Previous data showed that the cellular proteins TIA-1 and TIAR bound specifically to West Nile virus 3′ minus-strand stem-loop [WNV3′(−)SL] RNA (37) colocalized with flavivirus replication complexes in WNV- dengue virus-infected cells (21). In present study, sites on WNV3′(−)SL required for efficient vitro T-cell intracellular antigen-related (TIAR) antigen-1 (TIA-1) protein binding were mapped short AU sequences (UAAUU) located two internal loops of structure. Infectious clone RNAs...
Nucleophosmin (NPM) is an important phosphoprotein with pleiotropic functions in various cellular processes. Although phosphorylation has been postulated as functional determinant, possible regulatory roles of this modification on NPM are not fully characterized. Here, we find that dephosphorylated threonine residues (Thr199 and Thr234/237) response to UV-induced DNA damage. Further experiments indicate the serine/threonine protein phosphatase PP1β a physiological under both genotoxic stress...
Death evasion is crucial for both carcinogenesis and resistance to anticancer therapies. Recently, we identified nucleophosmin (NPM) as a key factor counteracting death stimuli in human hepatocellular carcinoma (HCC) cells. Here report the identification of novel NPM-BCL2-associated X protein (BAX) pathway orchestrating HCC Silencing NPM expression significantly sensitized cells-particularly those bearing inactivated p53 gene (Huh7, Hep3B, Mahlavu)-to ultraviolet irradiation, mitomycin C,...
Abstract Background Esophageal squamous cell carcinoma (ESCC) is the sixth leading cause of cancer-associated death worldwide with a dismal overall 5-year survival rate less than 20%. The standard first-line therapy for advanced ESCC concomitant chemo-radiation (CCRT); however, patients usually develop resistance, resulting in unfavorable outcomes. Therefore, it urgent to identify mechanisms underlying CCRT resistance and effective treatment strategies. Methods Patients’ endoscopic biopsy...
Anxiety disorders significantly impair quality of life. However, limited knowledge the underlying mechanisms impedes development effective therapeutics. Previous studies have suggested that expression Nptx2 gene is associated with anxiety, but neurobiological processes this association remain unclear. We generated multiple mouse models knockout or overexpression in specific brain regions and during different developmental stages to assess adult neurogenesis, glucocorticoid-related...