A5056543644- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- Tissue Engineering and Regenerative Medicine
- Metastasis and carcinoma case studies
- Signaling Pathways in Disease
- Immune Response and Inflammation
- Liver physiology and pathology
- Advanced Breast Cancer Therapies
- PI3K/AKT/mTOR signaling in cancer
- Xenotransplantation and immune response
- Chronic Lymphocytic Leukemia Research
- Mesenchymal stem cell research
- Immune cells in cancer
- Lung Cancer Research Studies
- Organ Transplantation Techniques and Outcomes
- Cancer-related Molecular Pathways
- RNA Research and Splicing
- RNA modifications and cancer
- Heat shock proteins research
- Bipolar Disorder and Treatment
- Diabetes and associated disorders
- NF-κB Signaling Pathways
- Bladder and Urothelial Cancer Treatments
- Psoriasis: Treatment and Pathogenesis
Chang Gung Memorial Hospital
2021-2025
Linkou Chang Gung Memorial Hospital
2024
Chang Gung University
2024
E-Da Hospital
2014-2020
I-Shou University
2014-2019
Brigham and Women's Hospital
2009-2015
Harvard University
2011-2014
Institute of Molecular Biology, Academia Sinica
2006
St Vincent's Hospital
1998
Preclinical studies have shown that asparagine deprivation enhances T cell antitumour responses. Here we apply compassionate use of L-asparaginase, usually employed to treat blood malignancies, on patients with recurrent metastatic nasopharyngeal carcinoma. The L-asparaginase notably immune-checkpoint blockade therapy in by strengthening CD8+T fitness. Our study shows this combination is a promising avenue for clinical application and provides further mechanistic insight into how restriction...
The expression of CD127, the IL-7-binding subunit IL-7 R, is tightly regulated during development and activation T cells reduced chronic viral infection. However, molecular mechanism regulating dynamic CD127 still poorly understood. In this study, we report that transcription factor Ets-1 required for maintaining in murine peripheral cells. binds to activates promoter, its absence leads expression, attenuated signaling, impaired IL-7-dependent homeostatic proliferation strongly correlated...
Significance IL-2 plays critical roles in many immune responses. However, its transcriptional regulation is still not fully understood. Data described this paper demonstrate that the factor E26 transformation-specific sequence 1 (Ets-1) promotes expression of by nuclear activated T-cells (NFAT)-dependent mechanisms. Nuclear Ets-1 synergizes with NFAT transcription IL-2. also translocated to cytoplasm response calcium-induced signals and facilitates release from noncoding RNA repressor...
GATA-3, a C2C2-type zinc finger transcription factor, regulates many steps of T cell development and differentiation. It is also required for optimal production type 2 cytokines by CD8(+) cells. However, its role in the function this subset cells still poorly characterized. In paper, we report that GATA-3 MHC-mediated positive selection final maturation CD8 single-positive thymocytes. Deficiency mediated CD4cre transgene led to age-dependent lymphadenopathy partly because abnormal expansion...
PTPN22 is a tyrosine phosphatase and functions as damper of TCR signals. A C-to-T single nucleotide polymorphism (SNP) located at position 1858 human cDNA converting an arginine (R620) to tryptophan (W620) confers the highest risk rheumatoid arthritis among non-HLA genetic variations that are known be associated with this disease. The effect R-to-W conversion on activity protein impact minor T allele C1858T SNP activation cells has remained controversial. In addition, how overall regulated...
The integral membrane protein 2a (Itm2a) is one of the BRICHOS domain-containing proteins and structurally related to Itm2b Itm2c. It expressed preferentially in T lineage among hematopoietic cells induced by MHC-mediated positive selection. However, its transcriptional regulation function are poorly understood. Here we showed Itm2a be a target gene GATA-3, cell-specific transcription factor. Deficiency had little impact on development polyclonal but resulted partial defect thymocytes...
Radiotherapy (RT) combined with a radiosensitizer represents an important treatment for head and neck squamous cell carcinoma (HNSCC). Only few chemotherapy agents are currently approved as radiosensitizers targeted therapy. In this study, the potent cyclin-dependent kinase 4/6 (CDK4/6) inhibitor LEE011 was tested potential to act during RT.RT enhancement by assessed in vitro clonogenic assay, flow cytometry, western blot variety of HNSCC lines. The line OML1 its radiation-resistant clone...
There is high risk of metastasis and recurrence in head neck squamous cell carcinoma (HNSCC) patients, especially for patient who received definitive surgery adjuvant radiotherapy. Aberrant activation PI3K/AKT/mTOR signaling occurs approximately 80% HNSCC, which has been indicated to serve as prognostic biomarkers patients suffer from or metastasis. Therefore, this study, we focus on the relationship between expression level factors pathway tumor HNSCC patients. A tissue microarray (TMA) was...
Other SectionsABSTRACTINTRODUCTIONRESULTSDISCUSSIONMATERIALS AND METHODSACKNOWLEDGEMENTSCONFLICTS OF INTERESTFIGURESREFERENCES
Abstract Background Microsatellite instable (MSI) gastric cancers exhibit reduced lymph node (LN) metastasis and improved survival compared to microsatellite stable (MSS) counterparts. However, our longstanding observation, clinical N-staging (cN) is frequently overestimated in MSI cases. The implications underlying mechanisms of this discrepancy warrant further investigation. Materials methods We conducted a comprehensive review clinicopathological data from 141 1119 MSS cancer patients....
Nasopharyngeal carcinoma (NPC) is an Epstein-Barr virus (EBV)-associated cancer, and immune checkpoint inhibitors (ICIs) have shown efficacy in its treatment. The combination of chemotherapy ICIs represents a new trend the standard care for metastatic NPC. In this study, we aim to clarify cell profile related prognostic factors ICI-based treatment Programmed death ligand 1 (PD-L1) expression was measured 81 tissue samples that had not received prior ICI combined positive score (CPS) 58.0%...
C-Maf plays an important role in regulating cytokine production TH cells. Its transactivation of IL-4 is optimized by phosphorylation at Tyr21, Tyr92, and Tyr131. However, the molecular mechanism its tyrosine remains unknown. In this study, we demonstrate that Tec kinase family member Tec, but not Rlk or Itk, a c-Maf enhances c-Maf-dependent promoter activity. This effect counteracted Ptpn22, which physically interacts with facilitates dephosphorylation thereby attenuating transcriptional We...
With ageing populations, new elderly end-stage kidney disease (ESKD) cases rise. Unlike younger patients, ESKD patients are less likely to undergo transplant, and therefore the decision of receiving peritoneal dialysis (PD) hemodialysis (HD) is more crucial. A total 36,852 aged than 65, who were newly diagnosed with initiated renal replacement therapy between 2013 2019 identified. These categorized into two groups: PD group HD according their long-term treatment. After propensity score...
Glossogyne tenuifolia (GT) (Hsiang-ju) is a Chinese herbal medicine previously exhibited an anti-inflammatory activity. This study aimed to investigate the effect of GT ethanol extract (GTE) on T cell-mediated adaptive immunity.Human peripheral blood mononuclear cells (PBMCs) and Jurkat were activated by phytohemagglutinin in presence various doses (3.13-50 μg/mL) GTE. The GTE cell activation was examined proliferation assay PBMCs level marker CD69 surface cells. Apoptosis determined...
Background/Aim: Chronic lymphocytic leukemia (CLL) still remains an incurable disease as the cells evade apoptosis, which is obstacle for current therapeutic approaches. Therefore, our aim was to identify ideal target of leukemic cell growth developing inhibitors. Materials and Methods: Mouse line L1210, human Toledo a DBA/2 mouse graft model were used analyze activity dual mTORC1/2 inhibitor AZD2014s. Western blotting flow cytometry performed determine mechanism. Results: AZD2014 inhibited...
Colorectal cancer (CRC) is one of the most common malignant tumors in world. This study aimed to investigate anticancer effect combination treatment Ribociclib (LEE011) and 5-Fluorouracil (5-FU) on CRC cells.HT-29 SW480 cells were treated with LEE011, 5-FU, or LEE011 5-FU. Cell viability cycle investigated through 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay flow cytometry. The expression cell cycle-related proteins was determined western...
In contrast to Western counties, the incidence of urothelial carcinoma (UC) remains mar-edly elevated in Taiwan. Regulatory T cells (Tregs) play a crucial role limiting immune responses within tumor microenvironment. To elucidate relationship between checkpoints microenvironment and UC progression, we utilize Gene Expression Omnibus (GEO) analyze microarray obtained from 308 patients with UC. We observed that expression level CD276 or TIM-3 was positively correlated late-stage poor...
While the transcription factor GATA-3 is well-established for its crucial role in T cell development, specific influence on invariant natural killer (iNKT) cells remains relatively unexplored. Using flow cytometry and single-cell transcriptomic analysis, we demonstrated that deficiency mice leads to absence of iNKT2 iNKT17 subsets, as well an altered distribution iNKT1 cells. Thymic iNKT lacking exhibited diminished expression PLZF T-bet, key factors involved differentiation, reduced...
Abstract Background Indoleamine 2,3-dioxygenase (IDO) impairs anti-pathogen and anti-tumour immunity. Mesenchymal stem cells (MSCs) modulate immunity via IDO but also suppress IFN-γ. While MSC induction by IFN-γ is established, other drivers in this immunosuppressive setting remain unknown. Methods Human bone marrow mesenchymal with or IL-10RA knockdown were co-cultured healthy donor T to assess immunosuppression. PDAC organoid anticancer activity was tested these co-cultures. Results...
The transcription factor Ets1 is essential for the development/differentiation of invariant Natural Killer T (iNKT) cells at multiple stages. However, its mechanisms action and target genes in iNKT are still elusive. Here, we show that required optimal expression Vα14Jα18 cell receptor (TCR) post-selected thymic their immediate differentiation. also critical maintaining peripheral homeostasis cells, which a role independent TCR. Genome-wide transcriptomic analyses further reveal controls...