A5079551707- Ion Channels and Receptors
- Phytochemicals and Antioxidant Activities
- Neurobiology and Insect Physiology Research
- Ion channel regulation and function
- Bioactive Compounds and Antitumor Agents
- Postharvest Quality and Shelf Life Management
- Biochemical Analysis and Sensing Techniques
- Respiratory and Cough-Related Research
- Herbal Medicine Research Studies
- Platelet Disorders and Treatments
- Cardiac electrophysiology and arrhythmias
- Circadian rhythm and melatonin
- Pharmacology and Obesity Treatment
- Blood Coagulation and Thrombosis Mechanisms
- Reproductive Biology and Fertility
- Pain Mechanisms and Treatments
- Endometriosis Research and Treatment
- Calcium signaling and nucleotide metabolism
- Pelvic floor disorders treatments
- Folate and B Vitamins Research
- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Sperm and Testicular Function
- Natural product bioactivities and synthesis
- Blood properties and coagulation
Universidad de Extremadura
2016-2025
Sorbonne Université
2018
Hôpital Charles-Foix
2018
Hospital San Pedro de Alcántara
2017
Inserm
2017
Université Paris-Sud
2017
Université Paris-Saclay
2017
Johannes Kepler University of Linz
2013-2016
Czech Academy of Sciences, Institute of Biophysics
2013
Cholesterol suppresses the activity of a channel needed to refill intracellular calcium stores.
Transient receptor potential channels convey signaling information from a number of stimuli to wide variety cellular functions, mainly by inducing changes in cytosolic Ca2+ concentration. Different members the TRPC, TRPM and TRPV subfamilies have been reported play role tumorigenesis. Here we show that estrogen positive triple negative breast cancer cell lines, MCF7 MDA-MB-231, respectively, exhibit enhanced expression TRPC6 channel as compared non-tumoral MCF10A line. In vitro knockdown...
Orai proteins contribute to Ca(2+) entry into cells through both store-dependent, release-activated (CRAC) channels (Orai1) and store-independent, arachidonic acid (AA)-regulated (ARC) leukotriene C4 (LTC4)-regulated (LRC) (Orai1/3 heteromultimers). Although activated by fundamentally different mechanisms, CRAC channels, like ARC LRC require stromal interacting molecule 1 (STIM1). The role of endoplasmic reticulum-resident STIM1 (ER-STIM1) in channel activation is widely accepted. ER-STIM1...
TRPC (canonical transient receptor potential) channel subunits have been shown to assemble into homo- or hetero-meric complexes, including different Ca2+-handling proteins, required for the activation of CCE (capacitative Ca2+ entry) NCCE (non-CCE) pathways. In present study we found evidence dynamic interaction between endogenously expressed hTRPC6 (human TRPC6) with either both Orai1 and STIM1 (stromal molecule 1) hTRPC3 participate in NCCE. Electrotransjection cells an anti-hTRPC6...
There is a body of evidence suggesting that Ca2+ handling proteins assemble into signaling complexes required for fine regulation signals, events regulate variety critical cellular processes. Canonical transient receptor potential (TRPC) and Orai have both been proposed to form Ca2+-permeable channels mediating entry upon agonist stimulation. A number studies demonstrated inositol 1,4,5-trisphosphate receptors (IP3Rs) interact with plasma membrane TRPC channels; however, at present there no...
AbstractStore-operated Ca2+ entry (SOCE) is a mechanism regulated by the filling state of intracellular stores that requires participation sensor STIM1, which communicates content to plasma membrane Ca2+-permeable channels. We have recently reported Orai1 mediates communication between STIM1 and channel hTRPC1. This event important confer hTRPC1 store depletion sensitivity, thus supporting functional role STIM1-Orai1-hTRPC1 complex in activation SOCE. Here we explored relevance lipid rafts...
Mammalian cells accumulate Ca2+ into agonist-sensitive acidic organelles, vesicles that possess a vacuolar proton-ATPase. Acidic stores include secretory granules and lysosome-related organelles. Current evidence clearly indicates participate in cell signaling function, including the activation of store-operated entry human platelets upon depletion stores, although mechanism underlying controlled by remains unclear. STIM1 has been presented as endoplasmic reticulum sensor, but its role...
The Ca(2+) release-activated channel mediates influx in a plethora of cell types, thereby controlling diverse cellular functions. complex is composed stromal interaction molecule 1 (STIM1), an endoplasmic reticulum Ca(2+)-sensing protein, and Orai1, plasma membrane channel. Channels STIM1 Orai1 mediate even at low extracellular concentrations. We investigated whether the activity adapted to different environmental used homology modeling molecular dynamics simulations predict presence...