A5016399856- Metabolomics and Mass Spectrometry Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Advanced Proteomics Techniques and Applications
- Bacterial Infections and Vaccines
- S100 Proteins and Annexins
- Immune cells in cancer
- Pneumonia and Respiratory Infections
- Immune Response and Inflammation
- Sepsis Diagnosis and Treatment
- Antimicrobial Peptides and Activities
- Diabetes and associated disorders
- Respiratory viral infections research
- Helminth infection and control
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- HIV-related health complications and treatments
- Mass Spectrometry Techniques and Applications
- Nanoplatforms for cancer theranostics
- HIV Research and Treatment
- Cell Image Analysis Techniques
- Antibiotics Pharmacokinetics and Efficacy
- Virology and Viral Diseases
- Pneumocystis jirovecii pneumonia detection and treatment
- Streptococcal Infections and Treatments
- Pancreatitis Pathology and Treatment
- Surgical site infection prevention
Lund University
2015-2025
Central Inland Fisheries Research Institute
2023
Odisha University of Agriculture and Technology
2017
Jeonbuk National University
2009
Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu
2005
Abstract Interstitial lung diseases such as idiopathic pulmonary fibrosis (IPF) are caused by persistent micro-injuries to alveolar epithelial tissues accompanied aberrant repair processes. IPF is currently treated with pirfenidone and nintedanib, compounds which slow the rate of disease progression but fail target underlying pathophysiological mechanisms. The DNA protein 8-oxoguanine glycosylase-1 (OGG1) has significant roles in modulation inflammation metabolic syndromes. Currently, no...
Abstract Neutrophils are crucial mediators of host defense that recruited to the central nervous system (CNS) in large numbers during acute bacterial meningitis caused by Streptococcus pneumoniae . release neutrophil extracellular traps (NETs) infections trap and kill bacteria. Intact NETs fibrous structures composed decondensed DNA neutrophil-derived antimicrobial proteins. Here we show cerebrospinal fluid (CSF) patients with pneumococcal meningitis, their absence other forms influx into...
•High levels of neutrophil extracellular traps (NETs) in the sputum severe COVID-19 patients.•Recombinant human DNase decreased NETs sputum.•Reduced were associated with recovery and improved oxygenation.•Mass spectrometry analyses plasma indicate resolution inflammation. Severe coronavirus disease 2019 (COVID-19) can result pneumonia acute respiratory failure. Accumulation mucus airways is a hallmark hypoxemia. Here, we show that quantitative proteome analysis from patients reveal high trap...
Abstract The incorporation of machine learning methods into proteomics workflows improves the identification disease-relevant biomarkers and biological pathways. However, models, such as deep neural networks, typically suffer from lack interpretability. Here, we present a approach to combine pathway analysis biomarker increase interpretability experiments. Our integrates priori knowledge relationships between proteins pathways processes sparse networks create biologically informed networks....
Serum Amyloid A (SAA) proteins are acute-phase reactants with critical roles in sterile and bacterial inflammation. Through vitro vivo experiments, we demonstrate that SAA amplify cytokine chemokine responses during inflammation enhance clearance infectious conditions. Mechanistically, augment NF-κB signaling, driving pro anti-inflammatory mediator production. SAA-/- mice carrying a deletion of the Saa1, Saa2, Saa3, Saa4 serum amyloid genes have better survival rates sepsis but more prone to...
Neutrophils are essential for host defense at the oral mucosa and neutropenia or functional neutrophil defects lead to disordered homeostasis. We found that neutrophils from harvested morning saliva had released extracellular traps (undergone NETosis) in vivo. The NETosis was mediated through intracellular signals elicited by binding of sialyl Lewis(X) present on salival mucins l-selectin neutrophils. This led rapid loss nuclear membrane release granule proteins with subsequent trap (NET)...
Pathogenic mycobacteria reside in, and are in turn controlled by, macrophages. However, emerging data suggest that neutrophils also play a critical role innate immunity to tuberculosis, presumably by their different antibacterial granule proteins. In this study, we purified neutrophil azurophil specific granules systematically analyzed the antimycobacterial activity of some proteins against M. smegmatis, bovis-BCG tuberculosis H37Rv. Using gel overlay colony forming unit assays showed...
Neutrophil extracellular traps (NETs) that are extensive webs of DNA covered with antimicrobial proteins into the environment during infection or inflammation as a part their defense arsenal. Image acquisition fluorescently labeled NETs and subsequent image-based quantification is frequently used to analyze NET formation (NETosis) in response various stimuli. However, there important limitations present methods for quantification. Manual tend be error-prone, tedious, often quite subjective,...
The plasma proteome is maintained by the influx and efflux of proteins from surrounding organs cells. To quantify extent to which different cells impact in healthy diseased conditions, we developed a mass-spectrometry-based proteomics strategy infer tissue origin detected human plasma. We first constructed an extensive atlas 18 vascularized 8 most abundant cell types blood. was interfaced with previous RNA protein atlases objectively define proteome-wide protein-organ associations enable...
Streptococcus pyogenes (Group A , GAS) is a significant human pathogen for which no licensed vaccine currently available. Here, we report de novo designed epitope-centric protein-based nanoparticle against GAS. By integrating structural mass spectrometry techniques and deep learning approaches, re-engineered protective epitope (D3m) present in domain 3 of streptolysin O, prominent pore-forming toxin produced by D3m was displayed on the surface self-assembling icosahedral (D3m-NP) to enhance...
Abstract Sepsis is the major cause of mortality across intensive care units globally, yet details accompanying pathological molecular events remain unclear. This knowledge gap has resulted in ineffective biomarker development and suboptimal treatment regimens to prevent manage organ dysfunction/damage. Here, we used pharmacoproteomics score time-dependent impact a murine Escherichia coli sepsis model after administering beta-lactam antibiotic meropenem (Mem) and/or immunomodulatory...
Wounds in the oral cavity, constantly exposed to both saliva and bacteria, heal quickly without infection. Furthermore, during licking of skin wounds, promotes wound healing plays a role keeping free infection.To investigate whether induces expression antimicrobial peptides (AMPs) human epidermal keratinocytes clearance intracellular bacteria these cells.Expression AMPs was investigated mucosa ex vivo injured by immunohistochemistry. Human beta-defensin-3 after stimulation, using real-time...
The complement system is activated in response to tissue injury. During wound healing, activation seems beneficial acute wounds but may be detrimental chronic wounds. We found that the epidermal expression of many components was only increased a minor extent skin vivo and cultured keratinocytes after exposure supernatant from stimulated mononuclear cells. In contrast, downregulated ex injured lacking stimulation infiltrating inflammatory cells with intact injury-induced growth factor...
Data independent acquisition mass spectrometry (DIA-MS) has recently emerged as an important method for the identification of blood-based biomarkers. However, large search space required to identify novel biomarkers from plasma proteome can introduce a high rate false positives that compromise accuracy discovery rates (FDR) using existing validation methods. We developed generalized precursor scoring (GPS) trained on 2.75 million precursors confidently control FDR while increasing number...
Abstract Sepsis is one of the leading causes mortality in world. Currently, heterogeneity sepsis makes it challenging to determine molecular mechanisms that define syndrome. Here, we leverage population scale proteomics analyze a well-defined cohort 1364 blood samples taken at time-of-admission emergency department from patients suspected sepsis. We identified panels proteins using explainable artificial intelligence predict clinical outcomes and applied these reduce high-dimensional data...
Meningitis is a potentially life-threatening infection characterized by the inflammation of leptomeningeal membranes. Many different viral and bacterial pathogens can cause meningitis, with differences in mortality rates, risk developing neurological sequelae, treatment options. Here, we constructed compendium digital cerebrospinal fluid (CSF) proteome maps to define pathogen-specific host response patterns meningitis. The results revealed drastic pathogen-type specific influx tissue-,...
Insulin-like growth factor (IGF)-1 has been successfully demonstrated to stimulate proteoglycan synthesis, slow down its catabolism and promote cartilage formation through well defined in vitro studies. It was therefore, assumed that IGF-1 would eventually serve augment current repair techniques vivo. Study designed determine the influence of with or without autografting. For this purpose articular model created left knee 48 New Zealand white rabbits either sex, 6-7 months old, weighing 1-2...
Many bacterial pathogens have developed methods to overcome the defences of host innate immune system. One such defence is release antimicrobial peptides (AMPs). Histones been found function as AMPs, in addition their main biological packaging and organising DNA into nucleosomes. In this study, Gram-positive anaerobic coccus <i>Finegoldia magna</i> was bind histones by Western blot immunoprecipitation analysis. <i>F. magna</i>, which normally a commensal skin mucous...
Streptococcal pharyngitis is among the most common bacterial infections, but molecular mechanisms involved remain poorly understood. Here we investigate interactions three major players in streptococcal pharyngitis: streptococci, plasma, and saliva. We find that saliva activates plasma coagulation system through both extrinsic intrinsic pathways, entrapping bacteria fibrin clots. The escape clots by activating host plasminogen. Our results identify a potential function for pathway of defense...
ABSTRACT The postmortem factors like rate of glycolysis and muscle shortening have a major role on meat characteristics these been efficiently manipulated by methods chilling regime, electrical stimulation different hanging. These optimized with time leaving little scope for further improvement. During the harvesting process there is generalized cell death often referred to as necrosis or apoptosis. This has well studied respect various physiological functions in vivo but its quality...
Abstract The plasma proteome is maintained by the influx and efflux of proteins from surrounding organs cells. To quantify extent different cells contribute to composition, we developed a mass spectrometry-based proteomics strategy infer origin detected in human health disease. First, constructed an extensive atlas 18 vascularized most abundant cell types blood. Second, was interfaced with previous RNA/protein atlases objectively define wide protein-organ associations enable both inference...
Table of contents P1 D-Dimer in adult patients with presumed sepsis and their clinical outcomes Surinder Kumar Sharma, Anurag Rohatgi, Mansi Bajaj P2 Diagnosis infection utilizing Acellix CD64 Charles L. Sprung, Ricardo Calderon Morales, Harvey Kasdan, Allon Reiter, Tobias Volker, Julien Meissonnier P3 High levels phenylcarboxylic acids reflect the severity ICU affect phagocytic activity neutrophils Natalia Beloborodova, Viktor Moroz, Aleksandra Bedova, Yulia Sarshor, Artem Osipov, Katerina...
Background Transrectal prostate biopsy (TRbx) transfers colonic bacteria into prostatic tissue, potentially causing infectious complications, including sepsis. Our objective was to determine whether needle shape, surface properties and sampling mechanism affect the number of transferred through colon wall, evaluate a novel with improved properties.Methods The standard Tru-Cut used today evaluated for mechanisms bacterial transfer in pilot study. A (Forsvall prototype) developed. TRbx...