A5100337696- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- Protein Hydrolysis and Bioactive Peptides
- Microbial infections and disease research
- Influenza Virus Research Studies
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Aquaculture disease management and microbiota
- Virology and Viral Diseases
- vaccines and immunoinformatics approaches
- Insect Utilization and Effects
- Animal Virus Infections Studies
- Respiratory viral infections research
- Invertebrate Immune Response Mechanisms
- Herpesvirus Infections and Treatments
- Coccidia and coccidiosis research
- Viral Infections and Vectors
- Probiotics and Fermented Foods
- Bacterial Infections and Vaccines
- Chemical Synthesis and Analysis
- Streptococcal Infections and Treatments
- Bacterial Genetics and Biotechnology
- Escherichia coli research studies
- T-cell and B-cell Immunology
- Antifungal resistance and susceptibility
Henan University of Science and Technology
2007-2021
Hubei Academy of Agricultural Sciences
2020
Animal Science Research Institute
2020
Dalian University of Technology
2015-2017
Northeast Agricultural University
2017
Dalian University
2017
Nanjing Agricultural University
2007-2014
Korea University of Science and Technology
2012
University of Science and Technology
2012
National Center for Nanoscience and Technology
2010
The function of Autoinducer-2 (AI-2) which acts as the signal molecule LuxS-mediated quorum sensing, is regulated through lsr operon (which includes eight genes: lsrK, lsrR, lsrA, lsrC, lsrD, lsrB, lsrF, and lsrG). However, functions remain unclear in avian pathogenic Escherichia coli (APEC), causes severe respiratory systemic diseases poultry. In this study, presence 60 APEC clinical strains (serotypes O1, O2, O78) was investigated found to be correlated with serotype has highest detection...
Abstract Escherichia coli, Pasteurella multocida, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella spp. and Staphylococc us aureus are six bacterial pathogens of avian. However, these may cause many similar pathological changes, resulting in clinical isolates that difficult to quickly simultaneously detect identify. Here, a multiplex polymerase chain reaction (m-PCR) assay is reported rapidly identify targets genes ( phoA, KMT1, ureR, toxA, invA , nuc ) samples. Six pairs specific...
Abstract Background The problem of increasing resistance against conventional antibiotics has drawn people’s attention. Therefore, the development novel antibacterial agents with effective and safe therapeutic effects is imminent. Antimicrobial peptides (AMPs) are considered a promising class due to their broad spectrum. Results In this study, on basis our previously studied peptide PMAP-37(F34-R), antimicrobial Chol-37(F34-R) was developed by N-terminal cholesterol modification increase...
Abstract Cathelicidins are short cationic host defense peptides and play a central role in innate immune system. Here we identified two novel cathelicidins, Cl-CATH2 3, from Columba livia . Evolutionary analysis of avian cathelicidins via phylogenetic tree Ka/Ks calculations supported the positive selection that prompted evolution CATH2 to CATH1 which originate common ancestor could belong one superfamily. 3 both adopt amphipathic α-helical comformations by circular dichroism 3D structures...
Abstract Cathelicidins are regarded as promising antibiotics due to their capability against antibiotic-resistant bacteria without cytotoxicity. However, some concerns about the balance of cytotoxicity and antimicrobial activity, weak stability enzymatic susceptibility sually restrict therapeutic use. Here, we designed a series shortened variants, Hc1~15, based on our previously characterized Hc-CATH. Hc3, one with best after point mutation was engineered trypsin inhibitor loop, ORB-C,...
With increasing resistance against conventional antibiotics, there is an urgent need to discover novel substances replace antibiotics. This provides opportunity for the development of antimicrobial peptides (AMPs). To develop new AMPs with effective and safe therapeutic effects, two PMAP-37 analogs called PMAP-37(R13-I) PMAP-37(K20/27-I) were designed increase hydrophobicity. Antimicrobial susceptibility testing animal infection models used assess their antibacterial activity. The results...
ABSTRACT There is an urgent need for identification of a new adjuvant capable selectively promoting efficient immune response use with vaccines and especially subunit vaccines. Our pervious study showed that Bursopentine (BP5) novel immunomodulatory peptide has the ability to significantly stimulate antigen-specific in mice. In this study, potential activities BP5 were examined chickens by coinjection inactivated avian influenza virus (AIV) (A/Duck/Jiangsu/NJ08/05 [AIV H9N2 subtype]). The...
Bacterial resistance induced by the use of antibiotics has provided a chance for development antimicrobial peptides (AMPs), and modification AMPs to enhance antibacterial activity or stability become research focus. PMAP‐37 is an AMP isolated from porcine myeloid marrow, studies on its have not yet been reported. In this study, three analogs named PMAP‐37(F9‐R), PMAP‐37(F34‐R), PMAP‐37(F9/34‐R) were designed residue substitution positive charge. The in vitro vivo detected. results showed...
Drug-resistant bacteria infections and drug residues have been increasing causing antibiotic resistance public health threats worldwide. Antimicrobial peptides (AMPs) are novel antimicrobial drugs with the potential to solve these problems. Here, a peptide based on our previously studied PMAP-36PW was designed via N-terminal myristoylation referred as Myr-36PW. The fatty acid modification provided as-prepared good stability higher activity compared in vitro. Moreover, Myr-36PW exhibited...
One hundred and 63 Bordetella bronchiseptica isolates from pigs, consisting of 65 isolated in 2003 98 2007, were tested for antimicrobial susceptibility to 17 agents by the disk diffusion method. All 163 B. sensitive polymyxin B cefoperazone/sulbactam; majority strains amikacin (149/163; 91.4%), gentamicin (132/163; 81.0%), ampicillin/sulbactam (127/163; 77.9%) ciprofloxacin (115/163; 70.6%). A high level resistance was found furazolidone (100%), ampicillin (90.2%), cefazolin (89.0%),...
Previous studies have proved that a novel antioxidant system composed of various peptides (AOPs) exists in the skin ranid frogs, keeping redox homeostasis. However, only small number AOPs been identified so far. Here, total 47 cDNA sequences encoding 21 different belonging to 11 families were cloned from library Limnonectes fragilis. Among them, fragilin-A1 (VKRRGQDCIHGFCSD) and fragilin-B1 (GQFNDKRWIPFG) also purified secretions. They selected with odorranain-Q-Lf (APIRMWYMYRKLTDMEPKPVA),...
Bursal Pentapeptide (BPP)-(III-V) are peptides isolated from the Bursa of Fabricius (BF).The peptides' adjuvant activities were evaluated in mice immunized with H9N2 avian influenza vaccine.The results showed that BPP-IV did not promote humoral or cellular immune responses. BPP-III had no effect on antibody production, but increased populations CD3+ and CD8+ T cells, CTL levels proliferation splenic T-lymphocytes. BPP-V level antibodies (HI, IgG its subtype IgG1) cytokine IL-4, as well ratio...
Abstract Background Pasteurella multocida is responsible for a highly infectious and contagious disease in birds, leading to heavy economic losses the chicken industry. However, pathogenesis of this poorly understood. We recently identified an aspartate ammonia-lyase ( aspA ) P. that was significantly upregulated under iron-restricted conditions, protein which could effectively protect flocks against . functions gene remain unclear. In present study, we constructed mutant strain △ :: kan...
Studies on the anticancer mechanism of peptide P18 in human leukemia K562 cells revealed that causes death most by depolarizing plasma membrane potential and enhancing permeability, rather than activating classical apoptosis pathway. The mechanistic studies indicate disrupting is an effective approach to kill cancer help design more analogues future therapies.