Gibeom Kim
A5102968997- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Ferroptosis and cancer prognosis
- Cancer Cells and Metastasis
- Cancer Research and Treatments
- FOXO transcription factor regulation
- Pancreatitis Pathology and Treatment
- Natural product bioactivities and synthesis
- Epigenetics and DNA Methylation
- Advanced Proteomics Techniques and Applications
- Magnetism in coordination complexes
- Seaweed-derived Bioactive Compounds
- Protein Degradation and Inhibitors
- Metal-Organic Frameworks: Synthesis and Applications
- Metal complexes synthesis and properties
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Bioactive Compounds and Antitumor Agents
Seoul National University
2020-2025
American Chemical Society
2015
Osaka University
2015
Spermatogenesis is a complex process of sperm generation, including mitosis, meiosis, and spermiogenesis. During spermiogenesis, histones in post-meiotic spermatids are removed from chromatin replaced by protamines. Although histone-to-protamine exchange important for nuclear condensation, the underlying regulatory mechanism still poorly understood. Here, we identify PHD finger protein 7 (PHF7) as an E3 ubiquitin ligase histone H3K14 spermatids. Generation Phf7-deficient mice Phf7 C160A...
The present study was performed to investigate the anti-inflammatory activity of Tetragonia tetragonoides hydrosols (TTH) and its underlying mechanism in lipopolysaccharide (LPS)-induced RAW 264.7 cells. Gas chromatography (GC) coupled with mass spectrometry retention index calculations showed that TTH were mainly composed tetratetracontane (29.5 %), nonacosane (27.6 oleamide (17.1 %). significantly decreased production nitric oxide (NO), prostaglandin E2 (PGE2), interleukin (IL)-6, IL-1β...
Tetranuclear nickel(II) and cobalt(II) complexes with μ3-O-3,5-dimethyl-1-pyrazylmethanolato (dmpm) μ-O,O′-acetato, [M4(dmpm)4(O2CCH3)4]·2CH3CN (M = Ni, Co), were isolated. The crystal structures determined by the single-crystal X-ray diffraction method at 90 K. Both of are isomorphous in tetragonal space group P42/n a 11.5812(11)Å, c 16.580(2)Å, V 2223.8(4)Å3, Dx 1.459 g/cm3, Z 2 for complex; these values 11.608(2)Å, 16.717(4)Å, 2252.4(7)Å3, 1.555 complex. R1 [I > 2σ(I)] wR2 (all data)...
Abstract The associated publication reports proteogenomic analysis of human pancreatic ductal adenocarcinoma (PDAC), where we provided significantly mutated genes (SMGs)/biomarkers, cellular pathways, and cell types as potential therapeutic targets to improve stratification patients with PDAC. This protocol describes the detailed methods for assays orthotopic PDAC mouse models, including tumour tissue processing, ultrasound imaging, Masson-trichrome staining, IHC immune markers.
Abstract The associated publication reports proteogenomic analysis of human pancreatic ductal adenocarcinoma (PDAC), where we provided significantly mutated genes (SMGs)/biomarkers, cellular pathways, and cell types as potential therapeutic targets to improve stratification patients with PDAC. This protocol describes the detailed methods for whole exome sequencing (WES) RNA analyses PDAC samples, including tissue processing analysis, DNA extraction, experimental procedures WES sequencing,...
Abstract The associated publication reports proteogenomic analysis of human pancreatic ductal adenocarcinoma (PDAC), where we provided significantly mutated genes (SMGs)/biomarkers, cellular pathways, and cell types as potential therapeutic targets to improve stratification patients with PDAC. This protocol describes the detailed methods for cell-based assays prognostic biomarkers in PDAC, including culture, viral transduction, assays.
Abstract The associated publication reports proteogenomic analysis of human pancreatic ductal adenocarcinoma (PDAC), where we provided significantly mutated genes (SMGs)/biomarkers, cellular pathways, and cell types as potential therapeutic targets to improve stratification patients with PDAC. This protocol describes the detailed methods for bioinformatics PDAC subtypes, including tumour purity estimation, subtype prediction samples in previous cohorts, pathway enrichment analysis, kinase...
Abstract The associated publication reports proteogenomic analysis of human pancreatic ductal adenocarcinoma (PDAC), where we provided significantly mutated genes (SMGs)/biomarkers, cellular pathways, and cell types as potential therapeutic targets to improve stratification patients with PDAC. This protocol describes the detailed methods for mass spectrometry-based proteomic PDAC samples, including sample preparation, liquid chromatography-tandem spectrometry (LC-MS/MS) analysis, data...
Pancreatic ductal adenocarcinoma (PDAC) is a devastating disease with poor prognosis, and the situation has not improved despite extensive clinical scientific research. Here, we report proteogenomic analysis of PDAC. Mutation-phosphorylation correlations identified signaling pathways associated somatic mutations in significantly mutated genes. mRNA-protein abundance revealed oncogene tumor suppressor candidates correlating patient survival. Integrated clustering mRNA, protein,...