To characterize mesenchymal stem cell-like cells isolated from human amniotic fluid for a new source of therapeutic cells.Fibroblastoid-type obtained at the time birth.The ability ex vivo expansion was investigated until senescence, and characteristics were analyzed by examining differentiation potential, messenger RNA expression immunophenotypes.A morphologically homogenous population fibroblastoid-type (HAFFTs) cells, similar to bone marrow (BM-MSCs), third passage. The became senescent...

Ischaemic stroke is a leading cause of death and long-lasting disability. Gastrodia elata blume (GEB) Chinese herb that widely used to treat convulsive disorders, such as epilepsy, p-hydroxybenzyl alcohol (HBA) the active ingredient in GEB. The present study was conducted evaluate effects GEB HBA on brain damage transcriptional levels Protein disulfide isomerase (PDI) 1-Cys peroxiredoxin (1-Cys Prx) genes known play role antioxidant systems after transient focal ischemia rat brain. Focal...

Abstract Various attempts have been made to develop stem cell-based therapy alleviate type I diabetes using animal models. However, it has a question whether human insulin produced from explanted cells is solely responsible for the normoglycemia of diabetic animals. In this study, we isolated neural crest-like eyelid fat and examined their therapeutic potentials diabetes. The adipose-derived (HEACs) displayed characteristics crest cells. Using two-step culture condition combined with...

The therapeutic goal in treating cerebral ischemia is to reduce the extent of brain injury and thus minimize neurological impairment. We examined effects p-hydroxybenzyl alcohol (HBA), an active component Gastrodia elata Blume, on transient focal ischemia-induced with respect involvement protein disulphide isomerase (PDI), nuclear factor-E2-related factor 2 (Nrf2), neurotrophic factors. All animals were ovariectomized 14 days before ischemic injury. Ischemic was induced for 1 h by middle...

Cells were isolated from four human amniotic membranes, and their biological characteristics analyzed during ex vivo expansion. Morphologically homogenous populations of fibroblast-like cells obtained the second or third passage. Under appropriate culture conditions, these membrane-derived mesenchymal (HAM) shown to differentiate into adipocytes, osteocytes, chondrocytes neuronal cells, as visualized by Oil Red O, von Kossa, alcian blue, anti-Neu N, anti-Gal C antibody staining,...

Relationship between Irritable Bowel Syndrome, Worry and Stress in Adolescent GirlsThe aim of this study is to investigate prevalence irritable bowel syndrome (IBS) among adolescents difference worry stress normal IBS groups.Questionnaire survey was conducted at a girl's middle high school.Students from seventh eleventh grade participated the examination on Rome II criteria, lifestyle dietary habits.Worry were measured with Korean version Penn State Questionnaire-Children Brief Encounter...

Advanced hepatic fibrosis is associated with cardiovascular disease (CVD) in patients nonalcoholic fatty liver (NAFLD). We investigated the association between noninvasive serum markers and coronary artery calcium score (CACS) subjects NAFLD.We analyzed 665 NAFLD without chronic or heart 2011 2015. The that were used to evaluate severity of included (NFS), fibrosis-4 (FIB-4) score, Forn's index, aspartate aminotransferase platelet ratio index (APRI).The areas under receiver operating...

Introduction. The neutrophil-lymphocyte ratio (NLR) is a significant systemic predictor of cardiovascular disease (CVD). coronary artery calcium score (CACS) reflects calcification and an independent risk factor for stenosis. In the present study, we explored relationship between NLR CACS in terms subclinical inflammation calcification. Materials Methods. We evaluated males females who did not have CVD, diabetes, high blood pressure, or fasting sugar levels. measured white cell, neutrophil,...

Dynamin, a GTP-binding protein, is involved in endocytosis animal cells. We found that dynamin-like ADL1, present multiple forms Arabidopsis leaf tissue. Subcellular fractionation experiments, together with gel-filtration and nondenaturing-gel electrophoresis revealed most of ADL1 as high-molecular-mass complex 400 to 600 kD the membrane or pellet fraction, whereas soluble fraction monomer. The subcellular distribution affected by various agents such Ca2+, cyclosporin A, GTP, ATP. Ca2+...

The study of the correlation menopausal symptoms with heart rate variability (HRV) has not been adequate. aim this was to investigate relationship between postmenopausal measured by menopause rating scale (MRS) and HRV.We assessed (using MRS) age, BMI, educational status, occupation, marital alcohol caffeine consumption, smoking history, exercise, duration sleep amenorrhea, degree anxiety depression, menarcheal variability. For evaluation HRV, record electrocardiogram for 5 minutes in...

Although fatigue is common in patients with inflammatory bowel disease (IBD), it often goes unrecognized and untreated. We investigated the degree of associated factors IBD. A multicenter study involving 147 IBD was conducted at five academic hospitals from August 2019 to December 2021. Fatigue evaluated using validated Korean version Multidimensional Inventory (MFI-K). Among 97 ulcerative colitis 50 Crohn's patients, mean total MFI-K score 59.0 ± 5.5, which corresponded a moderate-to-severe...

An increased homocysteine level is a risk factor for cardiovascular disease, venous thromboembolism, cerebrovascular and chronic kidney disease. In addition, vitamin D deficiency associated with coronary artery disease metabolic disorders. The present study included data from 1375 adults (895 men 480 women) mean age of 52.62 ± 9.94 years who visited the Health Promotion Center University Hospital in Gyeonggi-do, Republic Korea January 2018 to December 2022 routine checkups that assessments...

Coronary artery calcification (CAC), an indicator of coronary stenosis, is independent risk factor ischemic heart disease. Smoking increases the metabolic syndrome (MS) and cardiovascular Almost no previous studies have evaluated combined effect MS smoking status on CAC. Therefore, in this study we examined relationships between CAC, MS, smoking. This included 775 adult males without histories disease who visited Health Promotion Center at University Hospital Gyeonggi-do, Republic Korea from...

Introduction . A nonspecific symptom, fatigue accompanies a variety of diseases, including cancer, and can have grave impact on patients’ quality life. As for multidimensional instruments, one the most widely used is Multidimensional Fatigue Inventory (MFI). This study aims to verify reliability validity MFI Korean (MFI-K) version. Materials Method was performed at four university hospitals in Republic Korea. Among outpatients visiting Department Family Medicine, those complaining or chronic...

Background: Coronary artery disease (CAD) is an important issue in public health.Previous studies have shown that the ratio of fat to muscle mass a significant predictor metabolic disease, and it known be associated with atherosclerosis.In this study, we evaluated association between fat-to-muscle (FMR) CAD healthy adults.Methods: A total 617 participants without diabetes mellitus, hypertension, CAD, or stroke who visited Health Promotion Center from 2009 2018 were included study.Computed...

Ziprasidone is a benzisothiazolyl piperazine derivative that was developed from the chemically related antipsychotic drug tiospirone, and it improves neurological functions of ischemic brain effective in treatment schizophrenia. Mesenchymal stem cells (MSCs) are considered as leading candidate for regenerative therapy because their neural differentiation properties damaged brain. We investigated whether transplantation progenitor (NPCs) derived adipose mesenchymal combined with ziprasidone...

In this Study, we investigated the effects of lifestyle and metabolic syndrome on free oxygen radical levels in men women Korea.A total 254 adults were included study from February 2011 to June 2012 at a health promotion center. Information lifestyles presence factors was obtained. Biochemical markers measured radicals test (FORT) performed blood.Of subjects, 86 (33.9%) had syndrome, 187 (73.6%) men. Between subjects with without there significant increase alanine aminotransferase serum FORT...