Rheumatoid arthritis (RA) is a chronic inflammatory joint disease. Antioxidative treatment combined with chemotherapy holds great promise for RA treatment, and the ability to efficiently deliver drugs antioxidants synovial highly desired. Herein, we developed programmable polymeric microneedle (MN) platform transdermal delivery of methotrexate (MTX) reactive oxygen species (ROS) scavengers treatment. The biodegradable MNs made polyvinylpyrrolidone (PVP) were incorporated...

Developing an intelligent theranostic nanoplatform with satisfied diagnostic accuracy and therapeutic efficiency holds great promise for personalized nanomedicine. Herein, we constructed a smart nanodevice the accurate diagnosis of endogenous cancer microRNA (miRNA) biomarkers efficient photothermal therapy (PTT). The was composed polydopamine (PDA)-functionalized CuS nanosheets (CuS@PDA NSs) three elaborate DNA hairpin probes (TDHPs). CuS@PDA NSs acted as delivery vehicles agents. They...

COVID-19 is still a global public health concern, and the SARS-CoV-2 mutations require more effective antiviral agents. In this study, entry activity of thirty-one flavonoids was systematically evaluated by pseudovirus model. Twenty-four exhibited with IC50 values ranging from 10.27 to 172.63 µM SI 2.33 48.69. The structure-activity relationship these as inhibitors comprehensively summarized. A subsequent biolayer interferometry assay indicated that bind viral spike RBD block interaction...

MicroRNAs are endogenous, small non-coding RNAs that can play critical gene-regulatory roles during skeletal muscle development and highly conserved.miR-487b-3p is expressed in muscle, the detailed mechanism by which it regulates myoblast proliferation differentiation has not been explored.Here, we found miR-487b-3p expression was significantly higher goat tissues than other fetal mature tissues, suggesting an important effect on myogenesis.Functional studies showed overexpression suppressed...

Colon cancer is a common malignancy of the digestive tract with high morbidity and mortality. There an urgent need to identify effective biomarkers for early diagnosis colon prolong patient survival. Cyclins are family proteins that directly participate in cell cycle associated many types tumors, but role regulatory mechanism most cyclin members remain unclear. Here, we provide systematic comprehensive study gene expression their potential roles cancer. Pan-cancer analysis revealed genes...

Mitochondrial microRNAs (mitomiRs) critically orchestrate mitochondrial functions. Spatial imaging of mitomiRs is essential to understand its clinical value in diagnosis and prognosis. However, the direct monitoring living cells remains a key challenge. Herein, we report an AIE nanoreporter strategy for through pH-controlled exonuclease (Exo)-assisted target cycle signal amplification. The AIE-labeled DNA detection probes are conjugated on Exo III encapsulated polymeric nanoparticles (NPs)...

Cryopreserved cells stored in dry ice or liquid nitrogen is the classical method for transporting between research laboratories different cities around world order to maintain cell viability. An alternative ship live flasks filled with culture medium. Both methods have limitations of either a requirement on special shipping container short times survive process. We recently developed an agarose gel based directly adherent plates dishes ambient temperature. This convenient simplifies...

A novel β-glucosidase, which is inducible and capable of catalyzing the hydrolysis sennosides, was purified from Bifidobacterium sp. strain SEN with Triton X-100 solubilization DEAE-cellulose column chromatography, by hydrolytic activities toward sennoside B, 4-methylumbelliferyl β-glucoside (MUG), p-nitrophenyl (pNPG) were obtained together in same eluted fractions. The activity stable against detergents such as sodium dodecyl sulfate (SDS) X-100, but denatured SDS β-mercaptoethanal when...

A lipid second messenger, phosphatidylinositol (3,4,5)-trisphosphate (PIP3), is a signaling molecule that mediates central cellular events, such as growth, motility, and development by activating downstream proteins. Although functions of various PIP3 binding partners have been unveiled, the roles not resolved thoroughly because limitations analysis. Herein, we describe novel method for analysis relative amount based on spontaneous complementation split luciferase fragments. An N-terminal...

Combinatory photodynamic and chemotherapy have demonstrated superior performance in cancer ablation over singular therapeutics. However, therapy (PDT) often exhibits suboptimal efficacy for deep-seated tumors, owing to the limited penetration depth of illumination light, while is generally accompanied by severe side effects. Therefore, it imperative develop a functional nanoplatform combinatory PDT chemotherapy, which could, PDT, achieve enhanced light and, realize reduced therapeutic...

microRNAs are a kind of endogenous, non-coding, single-strand small RNA. They have been reported as an important regulatory factor in skeletal myogenesis. In this study, miR-452 was selected from RNA high-throughput sequencing data to explore its role Functionally, overexpression could promote C2C12 myoblast proliferation while inhibiting myogenic differentiation. On the contrary, inhibition suppress but accelerate Bioinformatics analysis and dual luciferase report assays showed that...

Fluorescent nanoparticles are promising tools for living cancer cell imaging and targeting. In this study, estrogen conjugated dye-doped fluorescent (estrogen FNPs) were synthesized characterized. The functionalized with low toxicity have shown high selectivity sensitivity toward target cells. Based on the specific recognition between receptor, FNPs been employed as optical probes targeting of receptor-positive cells fluorescence microscopy technology. results demonstrate that can...

A novel fluorescent imaging system was established to monitor ligand-receptor interactions. Fluorescent silica nanoparticles (FNPs) conjugated with ligand molecules were employed as ligands and molecular imprinting polymer (MIP) artificial receptors in this work. nano-particles which have controllable uniform size of 120 nm synthesized characterized by scanning electronic microscopy (SEM). The FNPs excitation emission peaks at 289 570 provide strong fluorescence intensity, excellent...

Traditional facial expression recognition includes a feature extractor and classifier. In this paper, multiple features fusion approaches for are proposed to improve the accuracy. We consider level method, serial fusion, decision linear opinion pool, combine features. Local binary patterns, local directional number pattern edge orientation histograms used extract Then, extreme learning machine is as classifier classification. Experiments on JAFFE CK+ show method achieves better results.

Bifidobacterium sp. strain SEN was isolated and characterized by hydrolytic conversion of sennosides to sennidins (Akao et al., Appl. Environ. Microbiol., 60, 1041 (1994)). The sennoside-hydrolyzing capacity the disappeared following addition glucose media in spite good bacterial growth potent activity hydrolyzing p-nitrophenyl β-D-glucopyranoside (pNPG). In a fructose-containing medium, no such suppressing effect shown. Following 10 h incubation 50 mM potassium phosphate buffer (pH 7.4),...

Since the recent emergence of swine and avian flu, there has been a sense urgency to discover new anti-influenza drugs. In this study, stable cell line with M2 ion channel/ enhanced Green fluorescent protein (EGFP) co-expression was established in order develop an EGFP-based high-throughput assay for screening channel inhibitors. This directly monitors proton conductivity by measuring fluorescence intensity EGFP, which is dependent on sensitive pH. The ability amantadine inhibit detected...

Traditional facial expression recognition includes a feature extractor and classifier. In this paper, multiple features fusion approaches for are proposed to improve the accuracy. We consider level method, serial fusion, decision linear opinion pool, combine features. Local binary patterns, local directional number pattern edge orientation histograms used extract Then, extreme learning machine is as classifier classification. Experiments on JAFFE CK+ show method achieves better results.