A5084383853- Neuroscience and Neuropharmacology Research
- Mitochondrial Function and Pathology
- Lipid Membrane Structure and Behavior
- Ion Channels and Receptors
- Neuroscience of respiration and sleep
- Adenosine and Purinergic Signaling
- Aluminum toxicity and tolerance in plants and animals
- Ion channel regulation and function
- Urinary Bladder and Prostate Research
- Metabolism and Genetic Disorders
- Retinal and Optic Conditions
- Cellular transport and secretion
- ATP Synthase and ATPases Research
- Acute Ischemic Stroke Management
- Ocular Diseases and Behçet’s Syndrome
- Vasculitis and related conditions
- Cerebrovascular and Carotid Artery Diseases
- Traumatic Brain Injury and Neurovascular Disturbances
- Marine animal studies overview
- Photoreceptor and optogenetics research
- Nicotinic Acetylcholine Receptors Study
- Epilepsy research and treatment
- Retinal Diseases and Treatments
- Angiogenesis and VEGF in Cancer
- Advanced MRI Techniques and Applications
Hospital de Egas Moniz
2018-2025
Centro Hospitalar de Lisboa Ocidental
2025
Universidade do Porto
2009-2024
University of Coimbra
2000-2017
Centro de Neurociências e Biologia Celular
2017
Hospitais da Universidade de Coimbra
2004-2016
University of Geneva
2006-2013
University of Aveiro
2003-2013
Carnegie Mellon University
2007
Patients with nephrotic syndrome are at heightened risk of infections due to the underlying disease pathophysiology and effects immunosuppressive therapies. Varicella-zoster virus (VZV) infection can cause severe complications in immunocompromised individuals. Concerns about safety live attenuated vaccines this population persist. Emerging vaccination strategies incorporate pre-vaccination stratification algorithms based on immunological criteria. We present a case five-year-old male...
We investigated the expression and functional status of TRPV1 receptor in human urothelial cells.Human urothelium was cultured studied by immunocytochemistry reverse transcriptase-polymerase chain reaction. The influence inflammatory mediators on mRNA levels also studied. Functional assays (cobalt uptake measurements whole cell voltage clamp records) were used to study response cells capsaicin, temperature, low pH mediators. Capsaicin induced adenosine triphosphate release from assessed...
Summary Objective Thirty percent of patients with epilepsy are refractory to medication. The majority these have mesial temporal lobe ( MTLE ). This prompts for new pharmacologic targets, like ATP ‐mediated signaling pathways, since the extracellular levels nucleotide dramatically increase during in vitro epileptic seizures. In this study, we investigated whether sodium‐dependent high‐affinity γ‐aminobutyric acid GABA ) and glutamate uptake by isolated nerve terminals human neocortex could...
Understanding how extant physiological landscapes arise from novel genetic interactions is key to elucidating phenotypic evolution. Sperm cells exemplify a striking case of functional compartmentalization shaped by molecular adjustments, notably regarding energy metabolism. Here, we examine the impact gene duplication and loss on evolution sperm energetics in mammals. Our findings reveal that acquisition an exclusive mechanism controlling plasma membrane Na+ gradient, critical for glucose...
Abstract Introduction: The association of acute anterior uveitis (AAU) with the HLA-B27 is well known but its implication on prognosis still controversial. purpose this study was to compare clinical features and outcomes HLA-B27-positive HLA-B27-negative AAU in a Portuguese population. Materials Methods: Medical records patients between January 2020 July 2021 were reviewed. typing, rheumatologic complications, gender, age, laterality, (fibrinous reaction hypopyon formation), ocular medical...
<h3>Objective</h3> To study the association among endothelial progenitor cells (EPCs), subacute blood–brain barrier (BBB) permeability, and clinical outcome after ischemic stroke, determining micro RNAs of EPCs responsible for good outcome. <h3>Methods</h3> We included consecutive patients with nonlacunar acute strokes in territory a middle cerebral artery ages between 18 80 years. Clinical was defined as modified Rankin Scale score at 3 months. Neuroimaging performed day 0 7 by MRI,...
Abstract Hippocampus mossy fibre terminals activate CA3 pyramidal neurons via two distinct mechanisms, both quantal and glutamatergic: (i) rapid excitatory transmission in response to afferent action potentials (ii) delayed prolonged release following nicotinic receptor activation. These processes were analysed here using rat hippocampus fibres synaptosomes. The relationships between synaptosome depolarisation glutamate established high‐KCl gramicidin challenges. Half‐maximal corresponded a...
In rapid synapses, neurotransmitter quanta are emitted in less than 100 mus, often at a high frequency. Using fast cryofixation of we found very brief (2-3 ms) change affecting intramembrane particles presynaptic membrane. Vesicle openings also occurred but after significant delay. The particle is most probably linked to mediatophore, proteolipid 220 kDa. Mediatophore aggregates were demonstrated active zones the Reconstituted liposomes, Xenopus oocytes, and neuroblastoma cells, mediatophore...
Abstract Aim Pathophysiological mechanisms of chronic visceral pain ( CVP ) are unknown. This study explores the association between sympathetic system and bladder nociceptors activity by testing effect a prolonged adrenergic stimulation on transient receptor potential vanilloid 1 TRPV 1) urothelial adenosine triphosphate ATP release. Methods Female Wistar rats received saline, phenylephrine PHE ), + silodosin, naftopidil or prazosin. knockout wild‐type mice saline . Visceral behaviour tests...
The model organism Neurospora crassa undergoes programmed cell death when exposed to staurosporine. Here we show that staurosporine causes defined changes in cytosolic free Ca2+ ([Ca2+]c) dynamics and a distinct Ca2+-signature involves influx from the external medium internal stores. We investigated molecular basis of this Ca2+-response with [Ca2+]c measurements combined pharmacological genetic approaches. Phospholipase C was identified as pivotal player during since modulation phospholipase...
We investigated the physiological role of vesicular Ca2+/H+ antiport in rapid synaptic transmission using Torpedo electric organ (a modified neuromuscular system). By inhibiting V-type H+-transporting ATPase (V-ATPase), bafilomycin A1 dissipates H+ gradient vesicles, thereby abolishing driving force. In electrophysiology experiments, significantly prolonged duration evoked electroplaque potential. A biochemical assay for acetylcholine (ACh) release showed that effect was presynaptic. Indeed,...
Spontaneous intracranial hypotension (SIH) is an important cause of new daily persistent headache. It thought to be due spontaneous spinal cerebrospinal fluid (CSF) leaks, which probably have a multifactorial etiology. The classic manifestation SIH orthostatic headache, but other neurological symptoms may present. An epidural blood patch the most effective treatment, blind infusion ineffective. We describe case young man who developed acute severe with pain worsening when assuming upright...
Habitat transitions have considerable consequences in organism homeostasis, as they require the adjustment of several concurrent physiological compartments to maintain stability and adapt a changing environment. Within range molecules with crucial role regulation different processes, neuropeptides are key agents. Here, we examined coding status their receptors pleiotropic activity Cetacea. Analysis 202 mammalian genomes, including 41 species Cetacea, exposed an intricate mutational landscape...
A low-affinity Ca²⁺/H⁺-antiport was described in the membrane of mammalian brain synaptic vesicles. Electrophysiological studies showed that this antiport contributes to extreme brevity excitation-release coupling rapid synapses. Synaptotagmin-1, a vesicular protein interacting with membranes upon Ca²⁺-binding, plays major role coupling, by synchronizing calcium entry fast neurotransmitter release. Here, we report synaptotagmin-1 is necessary for expression Ca²⁺/H⁺-antiport. We measured...
Alterations in the intracellular levels of calcium are a common response to cell death stimuli animals and fungi and, particularly, Neurospora crassa staurosporine. We highlight importance extracellular availability Ca2+ for this response. Limitation ion culture medium further sensitizes cells drug results increased accumulation reactive oxygen species (ROS). Conversely, an approximately 30-fold excess external leads tolerance lower ROS generation. In line with this, distinct...