A5012615383- Adenosine and Purinergic Signaling
- Biochemical and Molecular Research
- Adolescent and Pediatric Healthcare
- Genetics and Neurodevelopmental Disorders
- Peptidase Inhibition and Analysis
- Alkaline Phosphatase Research Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Neuroscience of respiration and sleep
- Neurological Complications and Syndromes
- Neonatal Health and Biochemistry
- Synthesis and Biological Evaluation
- Neonatal Respiratory Health Research
- Pancreatic function and diabetes
- Click Chemistry and Applications
- Nitric Oxide and Endothelin Effects
- Macrophage Migration Inhibitory Factor
- Gastroesophageal reflux and treatments
- Prenatal Substance Exposure Effects
- Immune Cell Function and Interaction
- Cardiovascular Issues in Pregnancy
- Sulfur-Based Synthesis Techniques
- RNA Interference and Gene Delivery
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Synthesis and Catalytic Reactions
Université Laval
2016-2025
Centre hospitalier de l'Université Laval
2016-2025
Université de Sherbrooke
1995-2024
Hudson Institute of Medical Research
2024
Centre hospitalier universitaire de Québec
2010-2022
Faculty (United Kingdom)
2015
University of Rostock
2015
Université du Québec
2012
University of Pittsburgh
2011
Beth Israel Deaconess Medical Center
1999-2009
Vascular ATP diphosphohydrolase (ATPDase) is a plasma membrane-bound enzyme that hydrolyses extracellular and ADP to AMP. Analysis of amino acid sequences available from various mammalian avian ATPDases revealed their close homology with CD39, putative B-cell activation marker. We, therefore, isolated CD39 cDNA human endothelial cells expressed this in COS-7 cells. was found have both immunological identity to, functional characteristics of, the vascular ATPDase. We also demonstrated ATPDase...
Mouse-to-rat cardiac transplants survive long term after transient complement depletion by cobra venom factor and T cell immunosuppression cyclosporin A. Expression of heme oxygenase-1 (HO-1) the graft vasculature is critical to achieve survival. In present study, we asked whether this protective effect was attributable generation one catabolic products HO-1, carbon monoxide (CO). Our data suggests that case. Under same immunosuppressive regimen allows mouse-to-rat (i.e., plus A), inhibition...
Myofibroblasts derived from portal fibroblasts are important fibrogenic cells in the early stages of biliary fibrosis. In contrast to hepatic stellate cells, have not been well studied vitro, and little is known about their myofibroblastic differentiation. this article we report isolation characterization rat culture. We demonstrate that primary undergo differentiation alpha-smooth muscle actin-expressing myofibroblasts over 10-14 days. Marker analysis comparing demonstrated these distinct...
CD39/ENTPD1 hydrolyzes proinflammatory nucleotides to generate adenosine. As purinergic mediators have been implicated in intestinal inflammation, we hypothesized that CD39 might protect against inflammatory bowel disease. We studied these possibilities a mouse model of colitis using mice with global deletion. then tested whether human genetic polymorphisms the gene influence susceptibility Crohn's induced Dextran Sodium Sulfate (DSS). Readouts included disease activity scores, histological...
Nucleoside triphosphate diphosphohydrolases 1, 2, 3 and 8 (NTPDases 8) are the dominant ectonucleotidases thereby expected to play important roles in nucleotide signaling. Distinct biochemical characteristics of individual NTPDases should allow them regulate P2 receptor activation differentially. Therefore, kinetic properties these enzymes were compared. efficiently hydrolyzed ATP UTP with K (m) values micromolar range, indicating that they terminate effects exerted by agonists at P2X(1-7)...
Abstract The presence of one or more calcium‐dependent ecto‐ATPases (enzymes that hydrolyze extracellular 5′‐triphosphates) in mammalian taste buds was first shown histochemically. Recent studies have established dominant consist enzymes now called nucleoside triphosphate diphosphohydrolases (NTPDases). Massively parallel signature sequencing (MPSS) from murine epithelium provided molecular evidence suggesting NTPDase2 is the most likely member present mouse papillae. Immunocytochemical and...
To investigate the role of adenosine formed extracellularly in vascular homeostasis, mice with a targeted deletion cd73/ecto-5'-nucleotidase were generated. Southern blot, RT-PCR, and Western blot analysis confirmed constitutive knockout. In vivo hemodynamic parameters revealed no significant differences systolic blood pressure, ejection fraction, or cardiac output between strains. However, basal coronary flow measured isolated perfused heart was significantly lower (-14%; P<0.05) mutant....
We have previously shown that the extracellular nucleoside triphosphate-hydrolyzing enzyme NTPDase2 is highly expressed in situ by stem/progenitor cells of two neurogenic regions adult murine brain:the subventricular zone (type B cells) and dentate gyrus hippocampus (residual radial glia). explored possibility multipotent neural stem express nucleotide receptors investigated their functional properties vitro. Neurospheres cultured from mouse SVZ presence epidermal growth factor fibroblast 2...
ARL 67156, 6-N,N-Diethyl-D-beta-gamma-dibromomethylene adenosine triphosphate, originally named FPL is the only commercially available inhibitor of ecto-ATPases. Since first report on this molecule, various ectonucleotidases responsible for hydrolysis ATP at cell surface have been cloned and characterized. In work, we identified inhibited by 67156.The effect 67156 recombinant NTPDase1, 2, 3 & 8 (mouse human), NPP1, NPP3 ecto-5'-nucleotidase (human) evaluated. The inhibition activity NTPDases...
The transient receptor potential channel, PKD2L1, is reported to be a candidate for sour taste based on molecular biological and functional studies. Here, we investigated the expression pattern of PKD2L1-immunoreactivity (IR) in buds mouse. PKD2L1-IR present few elongate cells each bud as previously. PKD2L1-expressing are different from those expressing PLCbeta2, marker Type II cells. Likewise PKD2L1-immunoreactive do not express ecto-ATPase which marks I PKD2L1-positive immunoreactive...
Adenosine is a neuromodulator acting through inhibitory A<sub>1</sub> receptors (A<sub>1</sub>Rs) and facilitatory A<sub>2A</sub>Rs, which have similar affinities for adenosine. It has been shown that the activity of intracellular adenosine kinase preferentially controls activation A<sub>1</sub>Rs, but source activating A<sub>2A</sub>Rs unknown. We now show ecto-5′-nucleotidase (CD73), major enzyme able to convert extracellular AMP into adenosine, colocalizes with in basal ganglia. In...
Macrophages are key elements in the inflammatory process, whereas depending on micro-environmental stimulation they exhibit a pro-inflammatory (classical/M1) or an anti-inflammatory/reparatory (alternative/M2) phenotype. Extracellular ATP can act as danger signal adenosine generally serves negative feedback mechanism to limit inflammation. The local increase nucleotides communication is controlled by ectonucleotidases, such members of ectonucleoside triphosphate diphosphohydrolase...
In the adult nervous system, multipotential stem cells of subventricular zone lateral ventricles generate neuron precursors (type-A cells) that migrate via rostral migratory stream to olfactory bulb where they differentiate into neurons. The migrating neuroblasts are surrounded by a sheath astrocytes (type-B cells). Using immunostaining, in situ hybridization and enzyme histochemistry, we demonstrate ecto-ATPase nucleoside triphosphate diphosphohydrolase 2 (NTPDase2) is expressed rat brain....
Abstract Extracellular nucleotides are ubiquitous extracellular mediators that interact with and activate nucleotide type 2 (P2) receptors. These receptors initiate a wide variety of signalling pathways appear important for functional associations between neurons glial cells the regulation blood flow, haemostatic inflammatory reactions in brain. Ectonucleotidases nucleotide‐metabolizing enzymes modulate P2 receptor‐mediated by regulated hydrolysis these agonists. A considerable number...