A5062683181- Reproductive System and Pregnancy
- Pregnancy and preeclampsia studies
- Reproductive Biology and Fertility
- Ectopic Pregnancy Diagnosis and Management
- Birth, Development, and Health
- Neonatal Respiratory Health Research
- Reproductive Physiology in Livestock
- Estrogen and related hormone effects
- Pregnancy and Medication Impact
- Preterm Birth and Chorioamnionitis
- Organ and Tissue Transplantation Research
- Cancer Risks and Factors
- Prenatal Screening and Diagnostics
- Endometriosis Research and Treatment
- COVID-19 Impact on Reproduction
- Assisted Reproductive Technology and Twin Pregnancy
- Growth Hormone and Insulin-like Growth Factors
- Ovarian function and disorders
- Hypothalamic control of reproductive hormones
- Global Maternal and Child Health
- Anesthesia and Neurotoxicity Research
- Effects and risks of endocrine disrupting chemicals
- Gestational Trophoblastic Disease Studies
- Gestational Diabetes Research and Management
- Maternal and Perinatal Health Interventions
University of Miami
2023-2025
Society for the Investigation of Early Pregnancy
2014-2023
Incept (United States)
2011-2023
Université de Versailles Saint-Quentin-en-Yvelines
2016-2023
Centre Hospitalier Intercommunal de Poissy
2016-2023
Yale University
1983-2017
Allgemeine Unfallversicherungsanstalt
2017
Spital Muri
2017
Università Cattolica del Sacro Cuore
2017
University Hospital of Bern
2017
Significance Secreted from viable embryos into the maternal circulation, PreImplantation factor (PIF) has long been implicated in modulating immune tolerance and promoting embryo implantation. Recent evidence also suggests its possible therapeutic use CNS disorders. However, molecular signal-transduction pathways driving PIF-mediated effects are unclear. Using murine cell culture combined with a rat hypoxic-ischemic brain injury model, we show that synthetic PIF (sPIF) inhibits microRNA...
Catechol-O-methyltransferase (COMT) characteristics and activity were studied using radioenzymatic techniques in placentas from normal high-risk patients at term. The affinity of the catechol estrogen 20-hydroxyestrone (20HE1), Km = 5 μM, for enzyme was found to be least 90-fold higher than that catecholamines orepinephrine, epinephrine, dopamine (450, 490, 850 respectively). product formed after incubation placental cytosol with tritiated 20HE! identified as being exclusively (3H)...
Abstract Aims Neutrophil trafficking within the vasculature strongly relies on intracellular calcium signalling. Sustained Ca2+ influx into cell requires a compensatory efflux of potassium to maintain membrane potential. Here, we aimed investigate whether voltage-gated channel KV1.3 regulates neutrophil function during acute inflammatory process by affecting sustained Methods and results Using in vitro assays electrophysiological techniques, show that is functionally expressed human...
Abstract Background PreImplantation Factor (PIF), a novel peptide secreted by viable embryos is essential for pregnancy: PIF modulates local immunity, promotes decidual pro-adhesion molecules and enhances trophoblast invasion. To determine the role of in post-fertilization embryo development, we measured peptide's concentration culture medium tested endogenous PIF's potential trophic effects direct interaction with embryo. Methods Determine levels multiple mouse single bovine cultured up to...
Viable embryos secrete preimplantation factor (PIF), a peptide that has autocrine effects where levels correlate with cultured development. sPIF (PIF synthetic analog) promotes implantation by regulating decidual-cells immunity, adhesion, apoptosis and enhances trophoblastic cell invasion. Herein priming on non-decidualized endometrium decidualized-stroma are investigated, assessing elements critical for effective embryo-maternal cross-talk, prior to at implantation. We tested effect human...
Bone marrow transplantation (BMT) to treat severe hematologic malignancies often leads potentially fatal acute graft-versus-host disease (GVHD), despite attempts at better donor-recipient matching and/or use of immunosuppressive agents. We report that embryo-derived PreImplantation Factor (PIF) plays a determining role in developing maternal/host tolerance toward the semiallogeneic or total allogeneic embryo and regulating systemic immune response. Synthetic PIF treatment has proven...
We have previously demonstrated that thyroid hormone synergizes with follicle-stimulating (FSH) to exert stimulatory effects on granulosa cell differentiation and function, suggesting it plays a physiological role in amplifying FSH-mediated of cells. The adequate these cells, followed by normal follicle development, is indispensable for ovulation subsequent corpus luteum formation. Thus, the present studies, clinical implications induction function were investigated. Serum levels total...
The cytologic localization and cellular levels of insulin receptors in the human ovary during follicular growth, regression atresia were examined by avidin/biotin immunoperoxidase techniques with a monoclonal antibody to receptor. In primordial follicles, only oocyte showed weak immunostaining for receptor, whereas stromal cells surrounding follicles moderately immunostained. earliest stage growth at which receptor granulosa theca interna became apparent was preantral stage. With increase...
Using a multichannel superfusion apparatus, the secretion of human chorionic gonadotropin (hCG) was measured at 1–6 minute intervals in placental explants 7–9 weeks gestation. hCG found to be secreted distinct pulses (4–5 fold above baseline) every 11–23 minutes peak peak. Addition one GnRH analog significantly increased, whereas progesterone decreased, pulsatile secretion.
Early identification of viable pregnancy is paramount for successful reproduction. Detection specific signals from pre-implantation embryos in normal circulation would indicate initiation embryo-maternal interaction and create a continuum to accurately reflect embryo/fetal well-being post-implantation. Viable mammalian secrete PreImplantation Factor (PIF), biomarker which plays key, multi-targeted roles promote implantation, trophoblast invasion modulate maternal innate adaptive immunity...
Preimplantation factor (PIF) is a peptide secreted by viable mammalian embryos. Moreover, it can be detected in the circulation of pregnant women. Recently, was shown that PIF promotes invasion trophoblast cell lines vitro. Successful human embryo implantation depends on deep and highly controlled extravillous (EVT) maternal endometrium. Trophoblast regulated part matrix metalloproteinase (MMP) activity integrin expression. The present study demonstrates presence early pregnancy...
Maternal control of inflammation is essential during pregnancy and an exaggerated response one the underlying causes fetal loss. Inflammatory mediated by multiple factors Toll-like receptors (TLRs) are central. Activation TLRs results in NALP-3 assembly apoptosis-associated speck-like protein containing a CARD (ASC) caspase-1 into inflammasome production pro-inflammatory cytokines IL-1β IL-18. Given that preventing measures lacking, we investigated PreImplantation Factor (PIF) as therapeutic...