A5100416611- Pharmacogenetics and Drug Metabolism
- Cellular transport and secretion
- Immune Response and Inflammation
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune cells in cancer
- Endoplasmic Reticulum Stress and Disease
- Ubiquitin and proteasome pathways
- Drug Transport and Resistance Mechanisms
- Autophagy in Disease and Therapy
- Traditional Chinese Medicine Studies
- Protein Hydrolysis and Bioactive Peptides
- Histone Deacetylase Inhibitors Research
- Signaling Pathways in Disease
- Healthcare and Venom Research
- Glycosylation and Glycoproteins Research
- Ginseng Biological Effects and Applications
- Traditional Chinese Medicine Analysis
- Glutathione Transferases and Polymorphisms
- Sphingolipid Metabolism and Signaling
- Adenosine and Purinergic Signaling
- Ion channel regulation and function
- RNA and protein synthesis mechanisms
- Dementia and Cognitive Impairment Research
- Genomics, phytochemicals, and oxidative stress
- Hormonal Regulation and Hypertension
Ajou University
2013-2025
Chungnam National University
2011-2021
Korea Basic Science Institute
2020
Sejong General Hospital
2013
Howard Hughes Medical Institute
2004-2012
Yale University
2004-2012
Kavli Institute for Theoretical Sciences
2012
North Carolina State University
2011
Rockefeller University
2004
Seoul National University
1998-2000
Plasma membrane phosphatidylinositol (PI) 4-phosphate (PtdIns4P) has critical functions via both direct interactions and metabolic conversion to PI 4,5-bisphosphate (PtdIns(4,5)P2) other downstream metabolites. However, mechanisms that control this PtdIns4P pool in cells of higher eukaryotes remain elusive. PI4KIIIα, the enzyme thought synthesize pool, is reported localize ER, contrary plasma localization its yeast homologue, Stt4. In paper, we show PI4KIIIα was targeted as part an...
Synaptojanin 1 is a polyphosphoinositide phosphatase concentrated in presynaptic nerve terminals, where it dephosphorylates pool of phosphatidylinositol 4,5-bisphosphate implicated synaptic vesicle recycling. Like other proteins with role endocytosis, synaptojanin undergoes constitutive phosphorylation resting synapses and stimulation-dependent dephosphorylation by calcineurin. Here, we show that cyclin-dependent kinase 5 (Cdk5) phosphorylates regulates its function both vitro intact...
Melatonin exhibits a wide variety of biological effects, including antioxidant and anti-inflammatory functions. Its role impedes the etiopathogenesis pancreatitis, but little is known about signaling pathway melatonin in induction enzymes acute pancreatitis (AP). The aim this study was to determine whether could prevent cerulein-induced AP through nuclear factor erythroid 2-related 2 (Nrf2) curtail inflammation by inhibition NF-kappaB. induced two intraperitoneal (i.p.) injections cerulein...
Abstract Chemokines play critical roles in inflammation by recruiting inflammatory cells to injury sites. In this study, we found that UDP induced expression of chemokines CCL2 (MCP-1) and CCL3 (MIP-1α) microglia, astrocytes, slice cultures activation P2Y6. Interestingly, was more highly expressed than CCL3. However, synthesis kinetics response differed microglia astrocytes; rapidly produced small amounts CCL2, whereas astrocytes continuously synthesized large resulting a high ultimate level...
The interaction of talin with phosphatidylinositol(4) phosphate 5 kinase type Iγ (PIPKIγ) regulates PI(4,5)P2 synthesis at synapses and focal adhesions. Here, we show that phosphorylation serine 650 (S650) within the talin-binding sequence human PIPKIγ blocks this interaction. At synapses, S650 is phosphorylated by p35/Cdk5 mitogen-activated protein rest, dephosphorylated calcineurin upon stimulation. also a substrate for cyclin B1/Cdk1 its in mitosis correlates adhesion disassembly....
Extracellular superoxide dismutase (SOD3) plays an important role in maintaining vascular redox homeostasis by eliminating superoxides. The angiotensin II (AngII) peptide mediates vasoconstriction part via reactive oxygen species (ROS) but has pathologic effects when elevated adults. Histone deacetylase 6 (HDAC6) modulates the acetylation of non‐histone substrates and is associated with hypertensive disorders. Here, we investigated potential regulation SOD3 HDAC6 human aortic endothelial...
Though Dieckol, a phlorotannin of Ecklonia cava, was known to have antioxidant, anticancer, antidiabetic, and anti-inflammatory effects, the underlying antifibrotic mechanism Dieckol still remains unclear until now. Thus, in current study, inhibitory on liver fibrosis elucidated mainly hepatic stellate cells (HSCs). exerted cytotoxicity LX-2, HSC-T6, HepG2 with reduced features large, spread out, flattened polygonal shapes LX-2 compared untreated control. attenuated expression α-SMA TGF-β1,...
Hypertension and endothelial dysfunction are associated with various cardiovascular diseases. Hydrogen sulphide (H2 S) produced by cystathionine γ-lyase (CSE) promotes vascular relaxation lowers hypertension. Honokiol (HNK), a natural compound in the Magnolia plant, has been shown to retain multifunctional properties such as anti-oxidative anti-inflammatory activities. However, potential role of HNK regulating CSE hypertension remains largely unknown. Here, we aimed demonstrate that...
The type I phosphatidylinositol 4-phosphate 5-kinase (PIP5K) family members and their lipid product, 4,5-bisphosphate (PIP2) are important regulators of actin cytoskeleton. PIP5Kγ 90 kDa (PIP5Kγ90), an isoform PIP5K, localizes to focal adhesions (FAs) is activated via its interaction with the cytoskeletal protein, talin. Currently, regulatory signaling pathways talin–PIP5Kγ90 related FA dynamics cell motility not well understood. Considering presence Akt consensus motifs in PIP5Kγ90, we...
The hexameric ATPase p97 has been implicated in diverse cellular processes through interactions with many different adaptor proteins at its N-terminal domain. Among these, the Ufd1-Npl4 heterodimer is a major adaptor, and p97-Ufd1-Npl4 complex plays an essential role endoplasmic reticulum-associated degradation (ERAD), acting as segregase that translocates ubiquitinated client protein from ER membrane into cytosol for proteasomal degradation. We determined crystal structure of domain SHP box...
Abstract Phosphatidylinositol 4‐phosphate 5‐kinase ( PIP 5K) family members generate phosphatidylinositol 4,5‐bisphosphate 2), a critical lipid regulator of diverse physiological processes. The 5K‐dependent 2 generation can also act upstream the oncogenic 3‐kinase PI 3K)/Akt pathway. Many studies have demonstrated various mechanisms spatiotemporal regulation 5K catalytic activity. However, there are few on protein stability. Here, we examined potential 5Kα, isoform, via ubiquitin‐proteasome...
Although propolis is one of the most popular functional foods for human health, there have been no comprehensive studies herb-drug interactions through cytochrome P450 (CYP) inhibition. The purpose this study was to determine inhibitory effects on activities CYP1A2, 2A6, 2B6, 2C9, 2C19, 2D6, 2E1 and 3A4 using pooled liver microsomes (HLMs). Propolis inhibited CYP2E1 CYP2C19 with an IC50 value 6.9, 16.8, 43.1 μg/mL, respectively, whereas CYP2A6, were unaffected. Based half-maximal...
Cardiac sodium channel dysfunction associated with the SCN5A gene presents mixed phenotypes, including long QT syndrome type 3, sinus node dysfunction, and dilated cardiomyopathy (DCM). We report a Korean case of an overlap cardiac channelopathy p.R1193Q polymorphism, treated by placement intrapericardial implantable cardioverter‐defibrillator (ICD) at age 27 months. Although patient received two appropriate life‐saving shocks for ventricular fibrillations, he eventually died DCM...
Alpha-fetoprotein (AFP) is a well-established serum biomarker for hepatocellular carcinoma (HCC) in clinical laboratories. However, AFP levels can often be high benign liver diseases such as cirrhosis. For this reason, specifically, the level of aberrant N-glycosylation has been proposed HCC to improve diagnostic performance using targeted mass spectrometry (MS). In study, we developed an endoglycosidase-assisted absolute quantification (AQUA) method by which measure N-glycosylated liquid...