A5070218669- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Lipid metabolism and biosynthesis
- Advanced Chemical Sensor Technologies
- Analytical chemistry methods development
- Fatty Acid Research and Health
- Atmospheric and Environmental Gas Dynamics
- CO2 Reduction Techniques and Catalysts
- Electrochemical sensors and biosensors
- Water Treatment and Disinfection
- Atmospheric chemistry and aerosols
- Electrochemical Analysis and Applications
- Analytical Chemistry and Sensors
- Toxic Organic Pollutants Impact
- Water Quality Monitoring and Analysis
- Cholesterol and Lipid Metabolism
- Hydrocarbon exploration and reservoir analysis
- RNA and protein synthesis mechanisms
- Physics of Superconductivity and Magnetism
- nanoparticles nucleation surface interactions
- Catalytic Processes in Materials Science
- Eicosanoids and Hypertension Pharmacology
- Gas Sensing Nanomaterials and Sensors
- Radioactive element chemistry and processing
University of Colorado Denver
2010-2019
University of Colorado Anschutz Medical Campus
2004-2018
Howard Hughes Medical Institute
2008
The University of Texas Southwestern Medical Center
2008
University of Colorado Health
1988-2007
National Jewish Health
2004
Trinity College Dublin
1999
University of Colorado Boulder
1977-1998
Cooperative Institute for Research in Environmental Sciences
1982-1998
National Renewable Energy Laboratory
1994-1996
The focus of the present study was to define human plasma lipidome and establish novel analytical methodologies quantify large spectrum lipids. Partial lipid analysis is now a regular part every patient's blood test physicians readily regularly prescribe drugs that alter levels major lipids such as cholesterol triglycerides. Plasma contains many thousands distinct molecular species fall into six main categories including fatty acyls, glycerolipids, glycerophospholipids, sphingolipids,...
Common organic matrix-assisted laser desorption/ionization (MALDI) matrices, 2,5-dihydroxybenzoic acid, 3,5-dimethoxy-4-hydroxycinnamic and α-cyano-4-hydroxycinnamic were found to undergo sublimation without decomposition under conditions of reduced pressure elevated temperature. This solid vapor-phase transition was exploited apply MALDI matrix onto tissue samples over a broad surface in solvent-free application for mass spectrometric imaging. Sublimation produced an even layer small...
The spectrum of nonalcoholic fatty liver disease (NAFLD) includes steatosis, steatohepatitis (NASH), and cirrhosis. Recognition timely diagnosis these different stages, particularly NASH, is important for both potential reversibility limitation complications. Liver biopsy remains the clinical standard definitive diagnosis. Diagnostic tools minimizing need invasive procedures or that add information to histologic data are in novel management strategies growing epidemic NAFLD. We describe an...
Recent developments in MALDI have enabled direct detection of lipids as intact molecular species present within cellular membranes. Abundant lipid-related ions are produced from the analysis thin tissue slices when sequential spectra acquired across a surface that has been coated with matrix. The lipid-derived can often be distinguished other biomolecules because significant mass defect these due to large number covalently bound hydrogen atoms hydrophobic molecules such lipids. Collisional...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectrochemical Reduction of CO2 Catalyzed by Small Organophosphine Dendrimers Containing PalladiumAlex Miedaner, Calvin J. Curtis, Robert M. Barkley, and Daniel L. DuBoisCite this: Inorg. Chem. 1994, 33, 24, 5482–5490Publication Date (Print):November 1, 1994Publication History Published online1 May 2002Published inissue 1 November 1994https://pubs.acs.org/doi/10.1021/ic00102a022https://doi.org/10.1021/ic00102a022research-articleACS...
Matrix-assisted laser desorption ionization/ionization imaging mass spectrometry (MALDI IMS) with a time-of-flight analyzer was used to characterize the distribution of lipid molecular species in brain rats two injury models. Ischemia/reperfusion rat after bilateral occlusion carotid artery altered appearance phospholipids present hippocampal region, specifically CA1 region. These regions also had large increase ion abundance at m/z 548.5 and collisional activation supported identification...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIdentification of methoxylated phenols as candidate tracers for atmospheric wood smoke pollutionSteven B. Hawthorne, David J. Miller, Robert M. Barkley, and Mark S. KriegerCite this: Environ. Sci. Technol. 1988, 22, 10, 1191–1196Publication Date (Print):October 1, 1988Publication History Published online1 May 2002Published inissue 1 October 1988https://pubs.acs.org/doi/10.1021/es00175a011https://doi.org/10.1021/es00175a011research-articleACS...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and characterization of alkaline-earth-metal .beta.-diketonate complexes used as precursors for chemical vapor deposition thin film superconductorsS. B. Turnipseed, R. M. Barkley, E. SieversCite this: Inorg. Chem. 1991, 30, 6, 1164–1170Publication Date (Print):March 1, 1991Publication History Published online1 May 2002Published inissue 1 March...
Neutral lipids are an important class of hydrophobic compounds found in all cells that play critical roles from energy storage to signal transduction. Several distinct structural families make up this class, and within each family there numbers individual molecular species. A solvent extraction protocol has been developed efficiently isolate neutral without complete more polar phospholipids. Normal-phase HPLC was used for the separation cholesteryl esters (CEs), monoalkylether...
Lipid mediators are important in lung biochemistry and derived from the enzymatic oxidation of arachidonic docosahexaenoic acids, which PUFAs that present phospholipids cell membranes. In this study, MALDI imaging MS was used to determine localization arachidonate- docosahexaenoate-containing mouse lung. These PUFA-containing were determined be uniquely abundant at lining small large airways, unequivocally identified by immunohistochemistry. addition, it found blood vessels characterized...
The positions of double bonds along the carbon chain methylene interrupted polyunsaturated fatty acids are unique identifiers specific derived from biochemical reactions that occur in cells. It is possible to obtain direct structural information as these bond using tandem mass spectrometry after collisional activation carboxylate anions an acetone adduct at each formed by photochemical Paternò-Büchi reaction with acetone. This can be carried out exposing a small portion inline fused silica...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSupercritical fluid transport-chemical deposition of filmsBrian N. Hansen, Brooks M. Hybertson, Robert Barkley, and E. SieversCite this: Chem. Mater. 1992, 4, 749–752Publication Date (Print):July 1, 1992Publication History Published online1 May 2002Published inissue 1 July 1992https://pubs.acs.org/doi/10.1021/cm00022a003https://doi.org/10.1021/cm00022a003research-articleACS PublicationsRequest reuse permissionsArticle...
The cell wall of M. tuberculosis is central to its success as a pathogen. Mycolic acids are key components this wall. genes involved in joining the alpha and mero mycolates located cluster, beginning with Rv3799c extending at least until Rv3804c. role each enzyme encoded by these five fairly well understood, except for Rv3802c. Rv3802 one seven putative cutinases genome tuberculosis. In phytopathogens, hydrolyze waxy layer plants, cutin. strictly mammalian pathogen, such tuberculosis, it...
Omega-3 polyunsaturated fatty acids (ω3 PUFAs) suppress inflammation through activation of free acid receptor 4 (FFAR4), but this pathway has not been explored in the context cardiovascular disease. We aimed to elucidate involvement FFAR4 by ω3 PUFAs process vascular and neointimal hyperplasia mice.We used mice with disruption (Ffar4(-/-)), along a strain that synthesizes high levels (fat-1) group crossed (Ffar4(-/-)/fat-1), role dysfunction using acute chronic thrombosis/vascular remodeling...
We hypothesized that measurement of a specific product from reaction N,N'-dimethylthiourea (Me2TU) and H2O2 would provide good indication the scavenging protection seen after addition Me2TU to biological systems. found yielded single stable product, dioxide. dioxide formation correlated with consumption as function added concentration was prevented by simultaneous catalase (but not boiled catalase), superoxide dismutase, dimethyl sulfoxide, mannitol, or sodium benzoate. formation,...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOzonization of caffeine in aqueous solutionKenneth J. Kolonko, Robert H. Shapiro, M. Barkley, and E. SieversCite this: Org. Chem. 1979, 44, 22, 3769–3778Publication Date (Print):October 1, 1979Publication History Published online1 May 2002Published inissue 1 October 1979https://pubs.acs.org/doi/10.1021/jo01336a007https://doi.org/10.1021/jo01336a007research-articleACS PublicationsRequest reuse permissionsArticle Views612Altmetric-Citations42LEARN...