The recent cloning of plant resistance (R) genes and the sequencing gene clusters have shed light on molecular evolution R genes. However, up to now, no attempt has been made correlate this with host-pathogen coevolution process at population level. Cross-inoculations were carried out between 26 strains fungal pathogen Colletotrichum lindemuthianum 48 Phaseolus vulgaris plants collected in three centers diversity host species. A high level for against was revealed. Most specificities...

ABSTRACT Population subdivision of Colletotrichum lindemuthianum, the causal agent anthracnose, was studied in three regions located centers diversity its host, Phaseolus vulgaris. Random amplified polymorphic DNA (RAPD) markers, restriction endonuclease analysis ribosomal internal transcribed spacer region, and virulence on a set 12 cultivars were used to assess genetic C. lindemuthianum strains isolated Mexican, Ecuadorian, Argentinean wild common bean populations. The significantly...

The invasive plant pathogen Xylella fastidiosa currently threatens European flora through the loss of economically and culturally important host plants. This emerging vector-borne bacterium, native to Americas, causes several diseases in a wide range plants including crops, ornamentals, trees. Previously absent from Europe, considered quarantine pathogen, X. was first detected Apulia, Italy 2013 associated with devastating disease olive trees (Olive Quick Decline Syndrome, OQDS). OQDS has...

Local adaptation, which has been detected for several wild pathosystems is influenced by gene flow and recombination. In this study, we investigate local adaptation population structure at a fine scale in populations of plant-pathogen fungus. We sampled hierarchically strains Colletotrichum lindemuthianum its host. The analysis AFLP patterns obtained 86 indicated that: (i) many different haplotypes can be discriminated, although occurrence recombination could not shown; (ii) migration...

This study investigated genetic polymorphism on a local scale in Puccinia striiformis f. sp. tritici populations during natural epidemics, four fields located northern France and sampled 1998 or 1999. Two hundred forty-seven isolates were analyzed for their amplified fragment length (AFLP) pattern through primer combinations, 194 of them also tested virulence factors. Only one two pathotypes found each field, all had v17, matching the recently introduced Yr17 resistance gene. Polymorphism...

Impact of host quantitative resistance on pathogen evolution is still poorly documented. In our study, we characterized the adaptation pathogenic fungus Colletotrichum gloeosporioides, to its host, water yam (Dioscorea alata). Genetic and diversities C. gloeosporioides populations were specified at field scale. We used nuclear markers describe fungal population structuring within between six fields three cultivars differently susceptible fungus. Strain aggressiveness was then quantified in...

Abstract Rosa damascena Mill. is used for essential oil production in many regions the world. Surveys conducted Lebanon showed that Lebanese people still widely use R. its multiple varieties to produce rose water. In this study seven cultivars of growing different ecosystems were collected from several Lebanon. The has been extracted by steam distillation and analyzed gas chromatography coupled mass spectrometry GC/MS equipped with a flame ionizing detector GC/FID. investigated factors...

The genetic structure of wild populations Colletotrichum lindemuthianum was evaluated for virulence and molecular markers. Forty‐five isolates were collected from five common bean located in their South‐Andean centre origin. pathogen monomorphic ITS regions, but 45 polymorphic markers identified using RAPDs. Polymorphism also observed; 15 pathotypes characterized on an international set 12 differentials. A variance analysis ( AMOVA ) showed that a very large part the total variation within...

Abstract Microsatellite markers were used to assess genetic diversity of Colletotrichum kahawae isolates from Burundi. Sampling was carried out through all coffee growing areas in The indices such as the number different alleles (Na), effective (Ne) revealed low with an average 1.900 and 1.196 respectively. Higher than expected heterozygosity significant linkage disequilibrium are consistent clonal reproduction. results showed a weak between populations region value Fst indicated that C....

Abstract Fifteen populations of wild bean ( Phaseolus vulgaris ), located in three provinces Argentina, were analysed for their polymorphism a complex resistance gene candidate (RGC) family clustered on the genome and phenotypes to strains Colletotrichum lindemuthianum. Results indicate that RGC is high. Population structure obtained markers related was compared population RAPD order infer evolutionary forces driving at both molecular phenotypic levels. Hierarchical analysis differentiation...

Studying spatial and temporal plant disease dynamics helps us to understand pathogen dispersal processes improve control recommendations. In this study, three cacao plots devoid of primary inoculum Phytophthora megakarya (causal agent black pod rot disease) upon establishment in 2006 were monitored for presence on a weekly basis from 2009 2016. Ripley’s K(r) function, join count statistics, Fisher’s Exact test used analyze dynamics. Disease distribution maps showed aggregated patterns all...

The genetic diversity and population structure of Mycosphaerella pinodes (Dydimella pinodes) in Algeria were investigated by AFLP markers. A total 75 isolates from different collection sites corresponding to four geographic regions analyzed. Data loci used estimate gene diversity, distances, make indirect measures flow between groups. Extensive was detected the M. regardless group. percentage polymorphic varied 41% semi-arid superior region 50% sub-humid region. Nei’s across 0.471 Shannon’s...

The cocoa black pod disease is caused by a fungus Phytophthora sp, which comprises two main species (Phytophthora palmivora and megakarya) commonly found in Ivorian plots cause significant damage. Despite numerous studies, this continues to spread even more so megakarya, did not exist Côte d'Ivoire, beginning significantly. This study focused on the evaluation of epidemiological factors responsible for progression megakarya Department Méagui, southwestern Cote d'Ivoire. To end, prospective...

Fungal plant parasites can have strong reproductive consequences on their hosts, but little is known about the amount of parasite-induced fitness loss under natural conditions. We present data from wild populations Colletotrichum lindemuthianum Sacc. & Magn., a fungal parasite that attacks common bean (Phaseolus vulgaris L.). estimated disease prevalence in two and virulence by examining naturally infected plants for three fitness-related traits: number seeds per pod, mass seed...