A5033790201- Mosquito-borne diseases and control
- Insect symbiosis and bacterial influences
- Viral Infections and Vectors
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Malaria Research and Control
- Hepatitis C virus research
- Viral gastroenteritis research and epidemiology
- Hepatitis B Virus Studies
- Virology and Viral Diseases
- Animal Virus Infections Studies
- Viral Infections and Immunology Research
- SARS-CoV-2 detection and testing
- Liver Disease Diagnosis and Treatment
- Viral Infections and Outbreaks Research
- Escherichia coli research studies
- Vibrio bacteria research studies
- Plant Virus Research Studies
- Vaccine Coverage and Hesitancy
- Studies on Chitinases and Chitosanases
Instituto Nacional de Salud
2018-2023
Technological University of Pereira
2022
Global surveillance programs for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are showing the emergence of variants with mutations in spike protein. Genomic and laboratory important to determine if these may be more infectious or less susceptible antiviral treatments vaccine-induced antibodies. Three most predominant SARS-CoV-2 Colombia during epidemiological peaks 2021 were isolated: Mu, a variant interest; Gamma, concern; B.1.111, which lacks genetic markers associated...
Strain monitoring for emergence of novel strains after the introduction rotavirus vaccine is an integral component routine immunization programs. Using a laboratory based strain surveillance system between 2008 and 2012, wide variation in pattern Colombia was founded both before monovalent 2009. G2P[4], fully heterotypic to predominant (47%) 2010 (54%), 2011 (86%), 2012 (32%). The presence this decreasing prevalence during most recent year suggests secular rather than pressure as cause...
Chikungunya virus (CHIKV) is considered a public health problem due to its rapid spread and high morbidity. This study aimed determine the genetic diversity phylogenetic relationships of CHIKVs in Colombia. A descriptive retrospective was carried out using sera patients infected with during outbreak The whole genomes CHIKV (n = 16) were sequenced an Illumina Hi-seq 2500 assembled Iterative Virus Assembler software. Bayesian inference analysis 157 strains worldwide origin. Colombian sequences...
Abstract During the last two decades, world has witnessed emergence and re-emergence of arthropod-borne viruses, better known as arboviruses. The close contact between sylvatic, rural peri-urban vector species humans been mainly determined by environment-modifying human activity. resulting interactions have led to multiple dead-end host infections allowed sylvatic arboviruses eventually adapt new vectors hosts, contributing establishment urban transmission cycles some viruses with enormous...
Colombia was the second most affected country during American Zika virus (ZIKV) epidemic, with over 109,000 reported cases. Despite scale of outbreak, limited genomic sequence data were available from Colombia. We sought to additional samples and use epidemiology describe ZIKV dynamics in Colombia.We sequenced genomes directly clinical diagnostic specimens infected Aedes aegypti selected cover temporal geographic breadth Colombian outbreak. performed phylogeographic analysis these genomes,...
Dengue is hyperendemic in Colombia, where a cyclic behavior of serotype replacement leading to periodic epidemics has been observed for decades. This level endemicity favors accumulation dengue virus genetic diversity and could be linked disease outcome. To assess the type 2 we sequenced envelope gene 24 isolates from acute cases or severe fever during period 2013-2016. The phylogenetic analysis revealed circulation Asian-American genotype Colombia that period, intra-genotype variability...
The family Tymoviridae comprises positive-sense RNA viruses, which mainly infect plants. Recently, a few Tymoviridae-like viruses have been found in mosquitoes, feed on vertebrate sources. We describe novel virus, putatively named, Guachaca virus (GUAV), isolated from Culex pipiens and quinquefasciatus species of mosquitoes collected the rural area Santa Marta, Colombia. After cytopathic effect was observed C6/36 cells, extracted processed through NetoVIR next-generation sequencing protocol,...
A Zika virus (ZIKV) strain was isolated from an acute febrile patient during the epidemics in Colombia. The intraperitoneally inoculated into BALB/c mice, and 7 days postinoculation, neurological manifestations ZIKV infection brain were demonstrated. reported genome sequence is highly related to strains circulating Americas.
Dengue is a mosquito-borne disease that of major importance in public health. Although it has been extensively studied at the molecular level, sequencing 5′ and 3′ ends untranslated regions (UTR) commonly requires specific approaches for completion corroboration. The present study aimed to characterize dengue virus types 1 4. twenty-nine isolates from acute infections were amplified through modified protocol rapid amplification cDNA approach. For end synthesis, anti-sense primers each...
Aedes aegypti is the primary vector of dengue, Zika, and chikungunya viruses. Studies have shown that insecticide resistance affects competence (VC) some mosquito species. This study evaluates effect to lambda-cyhalothrin kdr V1016I mutation genotypes on VC Ae . strains for DENV-2, ZIKV, CHIKV. Three with gradual (susceptible, resistant, highly resistant) were infected Individual mosquitoes tested detect virus infection in abdomen head-salivary glands, using RT-PCR, mutations allele-specific...
Abstract Background Colombia was the second most affected country during American Zika virus (ZIKV) epidemic, with over 109,000 reported cases. Despite scale of outbreak, limited genomic sequence data were available from Colombia. We sought to additional samples and use epidemiology describe ZIKV dynamics in Methods sequenced genomes directly clinical diagnostic specimens infected Aedes ae-gypti selected cover temporal geographic breadth Colombian outbreak. performed phylogeographic analysis...
Introducción. Se estima que 240 millones de personas en el mundo tienen infección crónica con virus la hepatitis B (HBV). En Colombia, endemia es variable y circulan diferentes genotipos virales. Las mutaciones a lo largo del genoma se han asociado resistencia antiviral, escape ante reacción anticuerpos neutralizadores tras vacunación o natural, oculta progresión carcinoma hepatocelular.Objetivo. Identificar los las presentes región codificante antígeno superficie (S) dominio transcriptasa...
Abstract Background By March 2022, around 34 million people in Colombia had received a complete scheme of vaccines against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) including, mRNA-based vaccines, viral vectored or inactivated whole virus vaccine. However, as several SARS-CoV-2 variants concern (VOC) and interest (VOI) co-circulate country, determining resistance level to vaccine-elicited neutralizing antibodies (nAbs) is useful improve efficacy COVID-19 vaccination...
Enteric viruses have been associated with the production of a variety diseases transmitted by fecal-oral route and carried through contaminated food water. Given their structure composition, they are highly resistant to environmental conditions most chemical agents used in purification processes. Therefore, systematic monitoring raw water is necessary ensure its quality especially when it for producing drinking human consumption. We identified presence rotavirus hepatitis A virus means...
Abstract The healthcare workers are considered as a high-risk group for infection with SARS-CoV-2, so they were included in the first stage of National Plan Vaccination against COVID-19 Colombia. An ongoing prospective cohort study to evaluate immune response vaccination 490 from health institutions Bogotá, Colombia, vaccinated between March and June 2021 BNT162b2 (Pfizer-BioNtech). Multiple samples collected during follow-up period 6 months after immunization. We report cases asymptomatic...
Hepatitis C Virus (HCV) infection is one of the leading risk factors for end-stage liver disease development worldwide. This RNA virus displays high genetic diversity with 8 genotypes and 96 subgenotypes heterogenous geographical distribution around world.In this study we carried out an active case finding individuals history transfusion events before 1996 in three cities Colombia. Then, characterization HCV genotypes, subgenotypes, resistance associate substitutions (RAS) was performed...
Summary Background Global surveillance programs for the virus that causes COVID-19 are showing emergence of variants with mutations in Spike protein, including Mu variant, recently declared a Variant Interest (VOI) by World Health Organization. Genomic and laboratory is important these types because they may be more infectious or less susceptible to antiviral treatments vaccine-induced antibodies. Objectives To evaluate sensitivity variant (B.1.621) neutralizing antibodies induced BNT162b2...