We report that human galectin-1 (dGal-1), a small dimeric β-galactoside-binding protein, induces phosphatidylserine (PS) exposure, measured by Annexin V staining, on promyelocytic HL-60 cells, T leukemic MOLT-4 and fMet-Leu-Phe-activated, but not resting, neutrophils. This effect of dGal-1 cells is enhanced pretreatment the with neuraminidase, treatment resting neutrophils neuraminidase does enhance their sensitivity to dGal-1. Although induction staining often associated apoptosis,...

We report our studies on the characterization of an ∼14-kDa lectin, termed galectin-1, that we have found to be expressed by Chinese hamster ovary (CHO) cells. cDNA for galectin-1 from CHO cells was prepared and sequenced, a recombinant form (rGal-1) in Escherichia coli. A mutated protein fully retained activity also constructed (termed C2SrGal-1) which Cys-2 changed Ser-2. rGal-1 stable presence reducing agent, but it quickly lost all absence agent. In contrast, glycoprotein ligands, such...

In the accompanying study (Cho, M., and Cummings, R. D.(1995) J. Biol. Chem. 270, 5198-5206), we reported that Chinese hamster ovary (CHO) cells synthesize galectin-1. We have now used several approaches to define subcellular location biosynthesis of galectin-1 in these cells. Galectin-1 was present on cell surface, as assessed by immunofluorescent staining with monospecific antibody protein. Quantitation surface-localized achieved metabolically radiolabeling [35S]Met/Cys measuring amount...

Galectin-1 (Gal-1) regulates leukocyte turnover by inducing the cell surface exposure of phosphatidylserine (PS), a ligand that targets cells for phagocytic removal, in absence apoptosis. Gal-1 monomer-dimer equilibrium appears to modulate Gal-1-induced PS exposure, although mechanism underlying this regulation remains unclear. Here we show sensitivity oxidation. A mutant form Gal-1, containing C2S and V5D mutations (mGal-1), exhibits impaired dimerization fails induce while retaining...

Snail family proteins regulate transcription of molecules for cell-cell adhesion during epithelial-mesenchymal transition (EMT). Based on putative glycogen synthase kinase 3β (GSK-3β) phosphorylation sites within the Slug/Snail2, we explored significance GSK-3β-mediated in Slug/Snail2 expression EMT. Mutation GSK-3β (S92/96A or S100/104A) enhanced Slug/Snail2-mediated EMT properties E-cadherin repression and vimentin induction, compared with wild-type Slug/Snail2. S92/96A mutation inhibited...

The aerial parts of Artemisia capillaris (Compositae) have been used in traditional Korean medicine as a cholagogic, antipyretic, anti-inflammatory, and diuretic purposes. In our previous study, ethanolic extracts the plant demonstrated marked anti-inflammatory effect lipopolysaccharide (LPS)-stimulated RAW 264.7 cells (J. Soc. Appl. Biol. Chem., 2010, 53, 275-282). present capillarisin (CPS), flavone, main constituent A. capillaris, was examined for its activity cells. We found that CPS...

Beta-glucans, which existed in the cell walls of cereals, bacteria, and fungi, comprise a group β-d-glucose polysaccharides. We investigated effects four kinds beta-glucan, that are derived from barley, yeast, mushroom, euglena on wound healing. The migration viability keratinocyte or fibroblast were analyzed using vitro scratch healing assay, invasion MTT vivo assay. All beta-glucans had significant effect at 20 μM showed no toxicity dermal fibroblast. Moreover, treatment keratinocytes with...

Abstract Myricetin is a commonly found dietary flavonoid. In the present study, we investigated effects of myricetin on migration and invasion radioresistant lung cancer cells (A549‐IR). Transcriptome analysis A549‐IR identified several differentially expressed genes (DEGs) in compared to parental A549 cells. Functional enrichment revealed that most DEGs were linked with PI3K‐AKT signaling, proteoglycans, focal adhesion, ECM–receptor interactions. demonstrated enhanced migratory potential...

We previously reported that the four-transmembrane L6 family member 5 (TM4SF5) was highly expressed in hepatocarcinoma, induced morphological elongation and epithelial-mesenchymal transition, caused abnormal cell growth multilayers vitro tumor formation vivo. In this study, we identified a synthetic compound, 4'-(p-toluenesulfonylamido)-4-hydroxychalcone (TSAHC) antagonized both TM4SF5-mediated multilayer TM4SF5-enhanced migration/invasion. TSAHC treatment multilayer-growing cells to grow...

Keratinocyte-fibroblast interactions are critical for skin repair after injury. During the proliferative phase of wound healing, proliferation, migration and differentiation these cells major mechanisms leading to tissue remodeling. We have previously reported that glycitin, a soy isoflavone, stimulates dermal fibroblast proliferation; phytochemical, 4′,6,7-trimethoxyisoflavone (TMF), induces HaCaT keratinocyte cells. therefore investigated whether compounds display synergistic effects on...

Objective: Recognized as pathogen-associated molecular patterns (PAMPs), β-glucans, a naturally occurring heterogeneous group of polysaccharides, were investigated for their ability to accelerate wound healing in the form high water-retaining hydrogel dressing. Approach: Full-thickness wounds on dorsal side mice created using 5-mm biopsy punch treated with β-glucan-based 2 weeks. Standardized photographs site taken at regular time intervals calculate percentage closure. Tissues isolated from...

The thermodynamics of carbohydrate binding to the 14 kDa dimeric beta-galactoside-binding lectin galectin-1 (Gal-1) from Chinese hamster ovary cells and four galactose-specific plant lectins were investigated by isothermal titration microcalorimetry. Recombinant Gal-1 Escherichia coli, a Cys-->Ser mutant with enhanced stability (C2S-Gal-1), monomeric (N-Gal-1) studied along soybean agglutinin Erythrina indica, crystagalli, corollodendrum. Although pattern association constants was similar...

Galectin-1 is a β-galactoside-binding protein secreted by animal cells, and it exists in monomer−dimer equilibrium (Kd ≈ 7 μM). The function(s) of galectin-1 is(are) not yet defined, but dimerization divalency are presumably important. Crystal structures the mammalian dimer predict N- C-terminal interactions at subunit interface. To examine mechanism formation possibly generate active monomeric galectin-1, mutations were made C-termini recombinant hamster galectin-1. N-Gal-1 contains...

Seasonal changes in Perkinsus olseni infection and gametogenesis the Manila clam, Ruditapes philippinarum , were investigated over a 14‐mo period at commercial clam bed on Seonjaedo Island, off west coast of Korea. The prevalence, intensity, gametogenic stages histologically evaluated. prevalence monthly mean intensity ranged from 38 to 97% 0.77–2.48 4‐point scale. autumn was significantly higher than summer (2003 2004, P < 0.05). In 2003, timing spawning varied with respect level;...