A5033434305- Advanced Glycation End Products research
- Cell Adhesion Molecules Research
- Glycosylation and Glycoproteins Research
- Cancer-related gene regulation
- Melanoma and MAPK Pathways
- S100 Proteins and Annexins
- Immune cells in cancer
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Cancer Research and Treatments
- Cellular Mechanics and Interactions
- Inflammatory mediators and NSAID effects
- Liver physiology and pathology
- Cancer, Stress, Anesthesia, and Immune Response
- Carbohydrate Chemistry and Synthesis
- Nanoplatforms for cancer theranostics
- Hippo pathway signaling and YAP/TAZ
- Cancer-related Molecular Pathways
- Nanoparticle-Based Drug Delivery
- Galectins and Cancer Biology
- Microtubule and mitosis dynamics
- Genetic factors in colorectal cancer
- Cancer Cells and Metastasis
- Immune responses and vaccinations
- Chemical Synthesis and Analysis
The Wistar Institute
2019-2024
University of Miami
2015-2023
Seoul National University
2008-2015
Cancer Research Institute of the Slovak Academy of Sciences
2008
Here, we report that functional heterogeneity of macrophages in cancer could be determined by the nature their precursors: monocytes (Mons) and monocytic myeloid-derived suppressor cells (M-MDSCs). Macrophages are differentiated from M-MDSCs, but not Mons, immune suppressive, with a genomic profile matching M-MDSCs. Immune-suppressive activity M-MDSC-derived is dependent on persistent expression S100A9 protein these cells. also promotes M2 polarization macrophages. Tissue-resident-...
Triple-negative breast cancer (TNBC) is a highly aggressive and metastatic subtype, which generally untreatable once it metastasizes. We hypothesized that interfering with the Receptor for Advanced Glycation End-products (RAGE) signaling small molecule RAGE inhibitors (TTP488/Azeliragon FPS-ZM1) would impair TNBC metastasis fundamental mechanisms underlying tumor progression metastasis. Both TTP488 FPS-ZM1 impaired spontaneous experimental of models, reducing to greater degree than FPS-ZM1....
We previously reported that the four-transmembrane L6 family member 5 (TM4SF5) was highly expressed in hepatocarcinoma, induced morphological elongation and epithelial-mesenchymal transition, caused abnormal cell growth multilayers vitro tumor formation vivo. In this study, we identified a synthetic compound, 4'-(p-toluenesulfonylamido)-4-hydroxychalcone (TSAHC) antagonized both TM4SF5-mediated multilayer TM4SF5-enhanced migration/invasion. TSAHC treatment multilayer-growing cells to grow...
Summary Transmembrane 4 L six family member 5 (TM4SF5) plays an important role in cell migration, and focal adhesion kinase (FAK) activity is essential for homeostatic pathological migration of adherent cells. However, it unclear how TM4SF5 signaling mediates the activation cellular machinery, FAK activated during adhesion. Here, we showed that direct adhesion-dependent binding to causes a structural alteration may release inhibitory intramolecular interaction FAK. In turn, this activate at...
The EMT (epithelial–mesenchymal transition) is involved in fibrosis and cancer, regulated by different signalling pathways mediated through soluble factors, actin reorganization transcription factor actions. Because the tetraspan (also called tetraspanin) TM4SF5 (transmembrane 4 L6 family member 5) highly expressed hepatocellular carcinoma induces EMT, understanding how expression hepatocytes important. We explored mechanisms that induce whether impaired for inhibit acquisition of...
BRAF and MEK inhibitors (BRAFi MEKi) are actively used for the treatment of metastatic melanoma in patients with BRAFV600E mutation their tumors. However, development resistance to BRAFi MEKi remains a difficult clinical challenge limited therapeutic options available these patients. In this study, we investigated mechanism potential utility combination adoptive T-cell therapy (ACT) resistant BRAFi.Investigations were performed vitro vivo various human cell lines sensitive as well...
The receptor for advanced glycation end products (RAGE) is a multiligand transmembrane that can undergo proteolysis at the cell surface to release soluble ectodomain. Here we observed ectodomain shedding of RAGE critical its role in regulating signaling and cellular function. Ectodomain both human mouse was dependent on ADAM10 activity induced with chemical activators (ionomycin, phorbol 12-myristate 13-acetate, 4-aminophenylmercuric acetate) endogenous stimuli (serum ligands). Ectopic...
Background Women with breast cancer (BCa) experience heightened distress, which is related to greater inflammation and poorer outcomes. The s100 protein family facilitates the inflammatory response by regulating myeloid cell function through binding of Toll‐like receptor 4 for advanced glycation end products (RAGE). heterodimer s100A8/A9 RAGE ligand associated hastened tumor development metastasis. Previously, a 10‐week stress‐management intervention using cognitive behavioral therapy (CBT)...
Abstract Overexpression of transmembrane 4 L six family member 5 (TM4SF5), a four‐transmembrane L6 member, causes aberrant cell proliferation and angiogenesis, but the roles TM4SF5 in migration, invasion, tumor metastasis remain unknown. Using vitro hepatocarcinoma cells that ectopically or endogenously express vivo mouse systems, metastatic potentials were examined. We found expression facilitated invadopodia formation, MMP activation, eventually lung nude mice, suppression with its shRNA...
Four-transmembrane L6 family member 5 (TM4SF5) and its homolog L6, a tumor antigen, form four-transmembrane family. TM4SF5 expression causes uncontrolled cell proliferation angiogenesis. Although other genuine transmembrane 4 superfamily (TM4SF) members co-operate with integrins for migration, roles of in the cellular spreading migration are unknown. Using hepatocarcinoma clones that ectopically express TM4SF5, we found cross talks via an extracellular interaction between integrin alpha2...
// Seo Hee Nam 1 , Doyeun Kim 2 Mi-Sook Lee Doohyung Tae Kyoung Kwak Minkyung Kang 2,3 Jihye Ryu Hye-Jin Haeng Eun Song Jungeun Choi Gyu-Ho Sang-Yeob 4 Hwa Park Dae Gyu Hoon Kwon Tai Young Jean Paul Thiery 5,6,7 Sunghoon 1,2 and Jung Weon Interdisciplinary Program in Genetic Engineering, Seoul National University, Seoul, Republic of Korea Department Pharmacy, Medicinal Bioconvergence Research Center, Institute Pharmaceutical Sciences, College 3 Biomedical Medicine, University Ulsan, 5 Cancer...
In this study, we evaluated the ability of negatively charged bio-degradable nanoparticles, ONP- 302, to inhibit tumor growth. Therapeutic treatment with ONP-302 in vivo resulted a marked delay growth three different syngeneic models immunocompetent mice. 302 efficacy persisted depletion CD8+ T cells mice and also was effective immune deficient Examination effects on components microenvironment (TME) were explored. caused gene expression shift TAMs toward pro-inflammatory M1 type...
Two separate clinical studies of advanced hepatocarcinoma patients recently reported that the multikinase inhibitor sorafenib (nexavar) could extend survival only by 2-3 months. We also previously demonstrated 4'-(p-toluenesulfonylamido)-4-hydroxychalcone (TSAHC) blocks multilayer growth and migration mediated TM4SF5, which is highly expressed in approximately 80% Korean patients. Therefore, we wondered how TSAHC might be different from to deal with terms therapeutic characteristics...
Integrin-mediated adhesion to extracellular matrix proteins is dynamically regulated during morphological changes and cell migration. Upon adhesion, protein-protein interactions among molecules at focal adhesions (FAs) play major roles in the regulation of morphogenesis Although tyrosine phosphorylation paxillin critically involved adhesion-mediated signaling, significance Ser-85 mechanism by which it regulates migration remain unclear. In this study, we examined how affects FA formation We...
Protein-protein interactions and/or signaling activities at focal adhesions, where integrin-mediated adhesion to extracellular matrix occurs, are critical for the regulation of adhesion-dependent cellular functions. Although phosphorylation and molecules have been intensively studied, effects O-GlcNAc modification their Ser/Thr residues on functions largely unexplored. We investigated actin reorganization morphology rat insulinoma INS-1 cells after glucosamine (GlcN) treatment. found that...
e14553 Background: The tumor microenvironment (TME) plays a crucial role in growth and progression has significant influence on response to therapy. TME consists of myeloid-derived cells, stroma (e.g. fibroblasts extracellular matrix (ECM)), the vasculature that together support progression. Studies animal models humans show myeloid- derived cells such as myeloid suppressor (MDSCs) associated macrophages (TAMs) engage activities These also promote immune suppression blunts efficacy...
Abstract Ovarian cancer is characterized by an immunosuppressive tumor microenvironment (TME) maintained tumor-associated M2-like macrophages (TAMs) hindering anti-tumor responses and immunotherapy efficacy. An effective approach to target TAMs in ovarian treatment currently not available, but would be expected release pressure, enable a robust T cell response improve outcomes. Retinoblastoma protein (Rb) well-known suppressor regulator of proliferation. Accumulating evidence demonstrates...
Abstract Ovarian cancer remains a major health threat with limited treatment options available. It is characterized by immunosuppressive tumor microenvironment (TME) maintained tumor- associated macrophages (TAMs) hindering anti-tumor responses and immunotherapy efficacy. Here we show that targeting retinoblastoma protein (Rb) disruption of its LxCxE cleft pocket, causes cell death in TAMs induction ER stress, p53 mitochondria-related pathways. A reduction pro-tumor Rb high M2-type from TME...