A5024221416- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Malaria Research and Control
- Infectious Encephalopathies and Encephalitis
- Viral gastroenteritis research and epidemiology
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Virus-based gene therapy research
- Long-Term Effects of COVID-19
- SARS-CoV-2 and COVID-19 Research
- Monoclonal and Polyclonal Antibodies Research
- Epigenetics and DNA Methylation
- Virology and Viral Diseases
- Pancreatic and Hepatic Oncology Research
- Genetic and rare skin diseases.
- Vascular Malformations and Hemangiomas
- Pancreatitis Pathology and Treatment
- Autoimmune and Inflammatory Disorders
- Dermatologic Treatments and Research
- Wnt/β-catenin signaling in development and cancer
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Animal Virus Infections Studies
- Parasites and Host Interactions
- Research on Leishmaniasis Studies
- CAR-T cell therapy research
Universidade Federal do Rio de Janeiro
2008-2025
Hospital Geral de Bonsucesso
2011-2025
Hospital Universitário Clementino Fraga Filho
2018-2023
Fundação Oswaldo Cruz
1995-1998
Neurological and other systemic complications occur in adults with severe COVID-19. Here we describe SARS-CoV-2 infection complicated by neuroinvasion the post-mortem tissues of a child.We performed complete autopsy 14-month-old child who died COVID-19 pneumonitis. Histological sections multiple organs were stained haematoxylin eosin. Luxol fast blue staining for myelin immunohistochemistry selected areas brain. The presence was investigated immunostaining anti-spike protein antibody...
Introduction The dengue virus (DENV) is the etiological agent that causes fever illness, an arbovirus with a major endemic potential has become increasingly prevalent in Brazil and already been associated fatal cases children. DENV tropism for several organs, including lungs causing pulmonary complications. aim of this article was to evaluate inflammatory histopathological profile lung tissue three children infected DENV, which represents group more susceptible fatality due its incomplete...
Zika virus (ZIKV) is an emerging involved in recent outbreaks Brazil. The association between the and Guillain-Barré syndrome (GBS) or congenital disorders has raised a worldwide concern. In this work, we investigated rare case, which was associated with GBS spontaneous retained abortion. Using specific anti-ZIKV staining, identified placenta (mainly Hofbauer cells) several fetal tissues, such as brain, lungs, kidneys, skin liver. Histological analyses of organs revealed different types...
SUMMARY OBJECTIVE: This prospective study aimed to provide a comprehensive analysis of the methylation status two pivotal genes, CDKN2A/p16INK4A (cyclin-dependent kinase inhibitor 2A) and RB1 (retinoblastoma transcriptional corepressor 1), in breast cancer patients. METHODS: Samples were obtained from 15 women diagnosed with who underwent total mastectomy. DNA was extracted tumor, non-tumor tissue, peripheral blood (circulating cell-free DNA). The pattern collected on day mastectomy compared...
Immunotherapy can revolutionize anti-cancer therapy if specific targets are available. Immunogenic peptides encoded by cancer-specific genes (CSGs) may enable targeted immunotherapy, even of oligo-mutated cancers, which lack neo-antigens generated protein-coding missense mutations. Here, we describe an algorithm and user-friendly software named RAVEN (Rich Analysis Variable gene Expressions in Numerous tissues) that automatizes the systematic fast identification CSG-encoded highly affine to...
Coronavirus disease 2019 (COVID-19) was initially characterized as a respiratory illness. Neurological manifestations were reported mostly in severely affected patients. Routes for brain infection and the presence of virus particles situ have not been well described, raising controversy about how causes neurological symptoms. Here, we report autopsy findings 1-year old infant with COVID-19. In addition to pneumonitis, meningitis multiple organ damage related thrombosis, previous...
The first fatal case caused by the new genome type 7i is described in an 8-month-old boy requiring long-term respiratory support who developed Reye's syndrome, acute distress, and bronchiolitis obliterans with evolution. Adenovirus was detected nasopharyngeal secretions persistently positive during hospitalization. IgM IgG adenovirus antibody titers measured serum enzyme-linked immunoassay (EIA) were 1:32 1:800, respectively. Serum interleukins (IL) interferons (IFN) EIA as follows: IL-2,...
Sweet syndrome or acute febrile neutrophilic dermatosis is a recurrent and rare skin disease caused by the release of cytokines, with diverse possible etiologic causes. It presents clinically polymorphic lesions, fever, arthralgia, peripheral leukocytosis. In general, it associated infections, malignancy drugs. usually regresses spontaneously treatment primarily to control basic disease. The authors report case child 1 year 11 months who developed syndrome.
During Schistosoma mansoni infection, there is morphological evidence of involvement various hematopoietic growth factors, which cause eosinophil, neutrophil, megakaryocytic and erythroid extramedullary foci in the liver, lymph nodes omental mesenteric milky spots. While eosinophil metaplasia periphery hepatic granulomas roughly reproduced intensity medullary eosinopoiesis, neutrophil metaplasia, on contrary, was more intense during period depression bone marrow. This fact suggests that are...
Dengue virus (DENV) infection represents a worldwide public health concern and can cause damage to multiple organs, including the kidney. In this work, we investigated histopathological changes caused by dengue along with detection of inflammatory mediators, cytokines, cell expression patterns in renal tissue three fatal cases children. Hematoxylin Eosin staining was performed analyze these changes. Immunohistochemistry allowed for immunological markers tissues that were quantified further...
Introduction Dengue virus (DENV), the etiologic agent of dengue fever illness, represents a global public health concern, mainly in tropical and subtropical areas across globe. It is well known that this acute viral disease can progress to severe hemorrhagic stages some individuals, however, immunopathogenic basis development more forms by these patients yet be fully understood. Objective In context, we investigated characterized histopathological features as cytokine profile cell...
A Síndrome de Bloch-Sulzberger (Incontinência Pigmentar) é uma genodermatose rara, que afeta, principalmente, o sexo feminino, pois costuma ser letal em pacientes do masculino intraútero. Caracteriza-se, pelas manifestações dermatológicas, podendo também apresentar anomalias dentárias, oftalmológicas e neurológicas. As lesões cutâneas apresentam 4 fases distintas: vesiculosa, verrucosa, pigmentar atrófica; podem seguir sequência irregular, havendo até sobreposição das mesmas
AbstractWe present a comparative histopathological study of both acute and chronic human adenovirus pneumonia, with reference to the cellular extracellular matrix components. Seventeen lungs from autopsied patients whose ages ranged 2 60 months were studied. Adenovirus types 1, 2, 3, 5, 7 isolated 15 lung disease, other two pulmonary illness. The results indicated occurrence basic patterns interstitial pneumonia: (1) classic pattern (acute), characterized by necrosis degeneration many type...
Introduction: E-cadherin, encoded by the CDH1 gene, is a glycoprotein involved in cell adhesion, and methylation of can prevent protein expression favoring tumor invasion. This study investigated DNA extracted from non-tumor tissues breast cancer patients. In addition, human epidermal growth factor receptor-2 (HER-2), estrogen receptor (ER), progesterone (PR), marker proliferation Ki-67 (Ki-67) was analyzed immunohistochemistry. Methods: Samples were collected 15 women diagnosed with...
ABSTRACT Immunotherapy can revolutionize anti-cancer therapy if specific targets are available. Recurrent somatic mutations in the exome create highly neo-antigens. However, especially pediatric cancers oligo-mutated and hardly exhibit recurrent Yet, immunogenic peptides encoded by cancer-specific genes (CSGs), which virtually not expressed normal tissues, may enable a targeted immunotherapy of such cancers. Here, we describe an algorithm provide user-friendly software named RAVEN (Rich...
Solid pseudopapillary neoplasm of the pancreas (SPNP) is considered as a tumor with low malignant potential. Little known about how its molecular heterogeneity involved in pathogenesis. We aim to evaluate by immunohistochemistry (IH) and proteomics three macroscopically distinct areas. Tumor fragments were obtained from 12-year-old female patient. identified mass spectrometry (MS) 1,427, 5,786, 4,298 proteins for each sample, 1,337 being common all fragments. Several MS results...