A5039247920- Sarcoma Diagnosis and Treatment
- RNA modifications and cancer
- Cardiac tumors and thrombi
- Lymphoma Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- CAR-T cell therapy research
- Cancer Genomics and Diagnostics
- Prostate Cancer Diagnosis and Treatment
- Corporate Management and Leadership
- Prostate Cancer Treatment and Research
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- RNA Research and Splicing
- Corporate Social Responsibility and Sustainability
- Cancer, Lipids, and Metabolism
- Cell Adhesion Molecules Research
- Monoclonal and Polyclonal Antibodies Research
- Cellular Mechanics and Interactions
- Corporate Governance and Management
- Lung Cancer Treatments and Mutations
- T-cell and B-cell Immunology
- Protein Degradation and Inhibitors
- Machine Learning in Bioinformatics
- biodegradable polymer synthesis and properties
- Fiber-reinforced polymer composites
Ludwig-Maximilians-Universität München
2016-2022
University of Göttingen
2018
Technical University of Munich
2014
The prediction of protein sub-cellular localization is an important step toward elucidating function. For each query sequence, LocTree2 applies machine learning (profile kernel SVM) to predict the native in 18 classes for eukaryotes, six bacteria and three archaea. method outputs a score that reflects reliability prediction. has performed on par with or better than any other state-of-the-art method. Here, we report availability LocTree3 as public web server. server includes learning-based...
Ewing sarcoma is an undifferentiated small-round-cell sarcoma. Although molecular detection of pathognomonic EWSR1-ETS fusions such as EWSR1-FLI1 enables definitive diagnosis, substantial confusion can arise if diagnostics are unavailable. Diagnosis based on the conventional immunohistochemical marker CD99 unreliable due to its abundant expression in morphological mimics. To identify novel diagnostic markers for sarcoma, we performed comparative analyses 768 tumors representing 21 entities...
Ewing sarcoma (EwS) is an aggressive cancer characterized by chromosomal translocations generating fusions of the EWSR1 gene with ETS transcription factors (in 85% FLI1). EWSR1-FLI1 induces expression via binding to enhancer-like GGAA-microsatellites, whose activity correlates number consecutive GGAA-repeats. Herein we investigate role secretory neuropeptide CALCB (calcitonin-related polypeptide β) in EwS, which signals CGRP (calcitonin gene-related peptide) receptor complex, containing...
Abstract Pediatric malignancies including Ewing sarcoma (EwS) feature a paucity of somatic alterations except for pathognomonic driver-mutations that cannot explain overt variations in clinical outcome. Here, we demonstrate EwS how cooperation dominant oncogenes and regulatory germline variants determine tumor growth, patient survival drug response. Binding the oncogenic EWSR1-FLI1 fusion transcription factor to polymorphic enhancer-like DNA element controls expression MYBL2 mediating these...
Ewing sarcoma (EwS) is an aggressive childhood cancer likely originating from mesenchymal stem cells or osteo-chondrogenic progenitors. It characterized by fusion oncoproteins involving EWSR1 and variable members of the ETS-family transcription factors (in 85% FLI1). EWSR1-FLI1 can induce target genes using GGAA-microsatellites as enhancers.Here, we show that hijacks developmental factor SOX6 - a physiological driver proliferation progenitors binding to intronic GGAA-microsatellite, which...
Up to 30-40% of Ewing sarcoma (EwS) patients with non-metastatic disease develop local or metastatic relapse within a time span 2-10 years. This is in part caused by the absence prognostic biomarkers that can identify high-risk and thus assign them risk-adapted monitoring treatment regimens. Since cancer stemness has been associated tumour poor patient outcomes, we investigated current study potential SOX2 (sex determining region Y box 2) - major transcription factor involved development...
Ewing sarcoma (EwS) is an aggressive cancer displaying undifferentiated small-round-cell histomorphology that can be easily confused with a broad spectrum of differential diagnoses. Using comparative transcriptomics and immunohistochemistry (IHC), we previously identified BCL11B GLG1 as potential specific auxiliary IHC markers for EWSR1-FLI1-positive EwS. Herein, aimed at validating the specificity both in far larger independent cohort EwS (including EWSR1-ERG-positive cases) Furthermore,...
Oncogenesis of Ewing sarcoma (EwS), the second most common malignant bone tumor childhood and adolescence, is dependent on expression chimeric EWSR1‐ETS fusion oncogenes, often EWSR1‐FLI1 (E/F). E/F leads to dysregulation focal adhesions (FAs) enhancing migratory capacity EwS cells. Here, we show that, in cell lines tissue samples, adhesion kinase (FAK) expressed phosphorylated at Y397 an E/F‐dependent way involving Ezrin. Employing different as vitro models, found that key properties are...
Immunotherapy can revolutionize anti-cancer therapy if specific targets are available. Immunogenic peptides encoded by cancer-specific genes (CSGs) may enable targeted immunotherapy, even of oligo-mutated cancers, which lack neo-antigens generated protein-coding missense mutations. Here, we describe an algorithm and user-friendly software named RAVEN (Rich Analysis Variable gene Expressions in Numerous tissues) that automatizes the systematic fast identification CSG-encoded highly affine to...
In prostate adenocarcinoma (PCa), distinction between indolent and aggressive disease is challenging. Around 50% of PCa are characterized by TMPRSS2‐ERG (T2E)‐fusion oncoproteins defining two molecular subtypes (T2E‐positive/negative). However, current prognostic tests do not differ both subtypes, which might affect outcome prediction. To investigate gene‐signatures associated with metastasis in T2E‐positive T2E‐negative independently, we integrated tumor transcriptomes clinicopathological...
Wood flour is particularly suitable as a filler in thermoplastics because it environmentally friendly, readily available, and offers high strength-to-density ratio. To overcome the insufficient interfacial adhesion between hydrophilic wood hydrophobic matrix, thermoplastic polymer was grafted from via surface-initiated activators regenerated by electron transfer-atom transfer radical polymerization (SI-ARGET ATRP). particles were modified with an ATRP initiator subsequently methyl acrylate...
Soft-tissue sarcomas are rare, heterogeneous, and often aggressive mesenchymal cancers. Many of them associated with poor outcome, partially because biomarkers that can identify high-risk patients lacking. Studies on limited by small sample-sizes rendering the identification difficult when focusing individual cohorts. However, increasing number publicly available 'omics' data opens inroads to overcome this obstacle. Here, we combine transcriptome analyses, immunohistochemistry, functional...
// Andreas Kirschner 1 , Melanie Thiede Franziska Blaeschke 1, 2 Günther H.S. Richter Julia S. Gerke 3 Michaela C. Baldauf Thomas G.P. Grünewald 3, 4, 5 Dirk H. Busch 6 Stefan Burdach * Uwe Thiel Laboratory for Functional Genomics and Transplantation Biology, Departments of Pediatrics Children’s Cancer Research Center, Klinikum rechts der Isar, Technische Universität München, Munich, Germany Immunotherapy, Dr. von Hauner Hospital, Medical center the LMU Pediatric...
ABSTRACT Cell lines have been essential for major discoveries in cancer including Ewing sarcoma (EwS). EwS is a highly aggressive pediatric bone or soft-tissue characterized by oncogenic EWSR1-ETS fusion transcription factors converting polymorphic GGAA-microsatellites (mSats) into neo-enhancers. However, further detailed mechanistic evaluation of gene regulation hindered the limited number well-characterized cell line models. Here, we present Sarcoma Line Atlas (ESCLA) comprising 18 with...
Abstract Ewing sarcoma is an undifferentiated bone-associated cancer. Although molecular detection of pathognomonic EWSR1-ETS fusions such as EWSR1-FLI1 enables definitive diagnosis, substantial confusion can arise if diagnostics are unavailable. Diagnosis based solely on the conventional immunohistochemical marker CD99 unreliable due to its abundant expression in morphological mimics. This study aimed identify novel diagnostic markers for sarcoma. We analyzed 768 microarrays representing 21...
ABSTRACT Immunotherapy can revolutionize anti-cancer therapy if specific targets are available. Recurrent somatic mutations in the exome create highly neo-antigens. However, especially pediatric cancers oligo-mutated and hardly exhibit recurrent Yet, immunogenic peptides encoded by cancer-specific genes (CSGs), which virtually not expressed normal tissues, may enable a targeted immunotherapy of such cancers. Here, we describe an algorithm provide user-friendly software named RAVEN (Rich...
ABSTRACT Ewing sarcoma (EwS) is an aggressive childhood cancer likely originating from mesenchymal stem cells or osteo-chondrogenic progenitors. It characterized by fusion oncoproteins involving EWSR1 and variable members of the ETS-family transcription factors (in 85% FLI1). EWSR1-FLI1 can induce target genes using GGAA-microsatellites (mSats) as enhancers. Here, we show that hijacks developmental factor SOX6 – a physiological driver proliferation progenitors binding to intronic GGAA-mSat,...