A5080252987- Microbial Natural Products and Biosynthesis
- Marine Sponges and Natural Products
- Lung Cancer Treatments and Mutations
- Bladder and Urothelial Cancer Treatments
- Lung Cancer Research Studies
- Urinary and Genital Oncology Studies
- Enzyme Production and Characterization
- Genomics and Phylogenetic Studies
- Cancer Immunotherapy and Biomarkers
- Neuroendocrine Tumor Research Advances
- Synthesis and Biological Activity
- Cancer Genomics and Diagnostics
- Ion Transport and Channel Regulation
- Electrolyte and hormonal disorders
- Colorectal Cancer Treatments and Studies
- Soft tissue tumor case studies
- Cardiac tumors and thrombi
- Traditional and Medicinal Uses of Annonaceae
- Occupational and environmental lung diseases
- Immune cells in cancer
- Ocular and Laser Science Research
- Ocular Oncology and Treatments
- Phytochemical compounds biological activities
- Histone Deacetylase Inhibitors Research
- Carbohydrate Chemistry and Synthesis
Roswell Park Comprehensive Cancer Center
2020-2025
Florida Atlantic University
2016-2022
Harbor Branch Oceanographic Institute
2016-2022
Tianjin Medical University General Hospital
2010-2022
Tianjin Medical University Cancer Institute and Hospital
2021
117th Hospital of People's Liberation Army
2021
Chinese People's Liberation Army
2021
Wuhan University
2013-2020
Tianjin Medical University
2006-2015
Wenzhou Central Hospital
2013
Marine natural products have become an increasingly important source of new drug leads during recent years. In attempt to identify novel anti-microbial by bioprospecting deep-sea Actinobacteria, three angucyclines, nocardiopsistins A-C, were isolated from Nocardiopsis sp. strain HB-J378. Notably, the supplementation rare earth salt Lanthanum chloride (LaCl3) fermentation HB-J378 significantly increased yield these angucyclines. The structures A-C identified 1D and 2D NMR HR-MS data....
The global prevalence of drug resistance has created an urgent need for the discovery novel anti-infective drugs. major source antibiotics in current clinical practice is terrestrial actinobacteria; less-exploited deep-sea actinobacteria may serve as unprecedented natural products. In this study, we evaluated 50 strains derived from diverse deep water sponges and environmental niches their anti-microbial activities against a panel pathogens including Candida albicans, Clostridium difficile,...
During our search for interesting bioactive secondary metabolites from mangrove actinomycetes, the strain Streptomyces sp. 219807 which produced a high elaiophylin yield of 4486 mg/L was obtained. A new derivative, halichoblelide D (1), along with seven known analogues 2-8 isolated and identified culture broth. Their chemical structures were determined by detailed analysis 1D 2D NMR HRMS data. The absolute configuration (1) confirmed comparing CD spectrum those reported analogues. Compounds...
Neuroendocrine bladder cancer (NEBC) is a rare but highly aggressive cancer, representing approximately 1% of urinary cancer. The most common NEBC small cell (SCBC), characterized by high rates recurrence, chemotherapy resistance, and early mortality. SCBC histologically identical to lung (SCLC) remains significantly understudied. Advances in next-generation sequencing techniques have partially elucidated the molecular characteristics identified druggable targets. This review compiles recent...
Abstract Introduction: Pan-urothelial carcinoma (panUCC) involves synchronous or metachronous urothelial of the bladder (UCB) and upper tract (UTUC). We sought to model panUCC using a triple knockout (TKO: Trp53; Rb1; Pten) genetically engineered mouse (GEMM). Methods: Using urothelium-specific Uroplakin 2-Cre ERT2 (Upk2-CreERT2), we generated novel TKO mice (Upk2-Cre ERT2; Trp53f/f; Rb1f/f; Ptenf/f). The were monitored for hematuria, urine retention, tumor formation, survival. PCR primers...
RET rearrangements are rare, and occur in 1%-2% of all non-small cell lung cancer (NSCLC) patients. Pralsetinib has a significant anti-tumor effect patients with advanced NSCLC rearrangement. Previous studies have confirmed the efficiency neoadjuvant target therapy for NSCLC. Herein we present case involving female patient who was diagnosed stage IIIA adenocarcinoma harbored KIF5B-RET rearrangement based on next-generation sequencing. Radiologic downstaging indicated after pralsetinib...
Invertebrates, particularly sponges, have been a dominant source of new marine natural products. For example, lasonolide A (LSA) is potential anticancer molecule isolated from the sponge Forcepia sp., with nanomolar growth inhibitory activity and unique cytotoxicity profile against National Cancer Institute 60-cell-line screen. Here, we identified putative biosynthetic pathway for LSA. Genomic binning metagenome revealed Gram-negative bacterium belonging to phylum Verrucomicrobia as...
PreQ0 base (7-cyano-7-deazaguanine, compound 1) is the biosynthetic precursor of queuosine-tRNA and important synthetic intermediate for bioactive compounds. It was obtained first time as a new natural product from mangrove actinomycete Streptomyces qinglanensis 172205, during course searching anti-cancer compounds marine microbes. showed anti-HeLa (IC50 = 62.0 μg/ml) anti-HepG2 80.6 activities, higher cytotoxicity than positive control, fluorouracil. Furthermore, it exhibited weak α...
Diffusion weighted imaging (DWI), with the applying of intravoxel incoherent motion model, has showed promising results in obtaining additional information about microperfusion and tubular flow associated morphologic changes chronic kidney diseases. The study aims to evaluate potential T2-weighted signal intensity (SI) DWI mono- bi-exponential models reflect serial on cisplatin (CP) induced rat renal fibrosis models.Magnetic resonance exams were performed prior 2 nd day, 4 th 6 8 week, 3 rd...
One novel brominated nocardiopsistin D (1) and two new sulfur-containing nocardiopsistins E–F (2–3) were identified from Nocardiopsis sp. HB-J378. The biosynthetic gene cluster ncd featuring a brominase was identified. Compounds 1–3 exhibited significant anti-methicillin-resistant Staphylococcus aureus (anti-MRSA) activities with minimum inhibitory concentrations (MICs) of 0.098, 3.125, 0.195 μg/mL, respectively. single bromination in 1 drastically enhanced the anti-MRSA activity by 128-fold...
Abstract Crizotinib showed significant antitumor effect in patients with advanced ROS1‐rearranged non‐small cell lung cancers (NSCLC). Most recently, many studies have explored the feasibility and efficacy of target therapy for perioperative application NSCLC. Here, we describe a female patient who was diagnosed stage IIIB adenocarcinoma exhibiting CCDC6‐ROS1 rearrangement by high‐throughput sequencing. The tumor lymph nodes durable response after treatment crizotinib. Given that...
Five new compounds 15R-17,18-dehydroxantholipin (1), (3E,5E,7E)-3-methyldeca-3,5,7-triene-2,9-dione (2) and qinlactone A–C (3–5) were identified from mangrove Streptomyces qinglanensis 172205 with “genetic dereplication,” which deleted the highly expressed secondary metabolite-enterocin biosynthetic gene cluster. The chemical structures established by spectroscopic methods, absolute configurations determined electronic circular dichroism (ECD). Compound 1 exhibited strong anti-microbial...
ABSTRACT Comparative genome sequencing analysis of a lincomycin-resistant strain Streptomyces coelicolor A3(2) and the wild-type identified novel mutation conferring high level lincomycin resistance. Surprisingly, new was an in-frame DNA deletion in genes SCO4597 SCO4598 , resulting formation hybrid gene linR. encode two histidine kinases, which together with SCO4596 encoding response regulator, constitute unique two-component system. Sequence indicated that these three their arrangement...
Neoadjuvant chemotherapy (NAC) followed by radical cystectomy is the standard-of-care for patients with muscle-invasive bladder cancer (MIBC). Defects in nucleotide excision repair (NER) are associated improved responses to NAC. Excision Repair Cross-Complementation group 3 (ERCC3) a key component of NER process. No inhibitors available treating cancer. We have developed an ex vivo cell-based assay 6-4 pyrimidine-pyrimidinone (6-4PP) removal as surrogate measure capacity human cell lines....
Approximately 80% of patients with advanced bladder cancer do not respond to immune checkpoint inhibitor (ICI) immunotherapy. Therefore, there is an urgent unmet need develop clinically relevant preclinical models so that factors governing immunotherapy responses can be studied in immunocompetent mice. We developed a line mouse triple knockout (TKO: Trp53, Pten, Rb1) urothelial carcinoma organoids transplanted into These tumors recapitulate the molecular phenotypes and heterogeneous observed...
Functional pancreatic neuroendocrine tumors (pNETs) rarely produce vasopressin. Here, we reported a case of pNET producing vasopressin in 78-year-old man with hyponatremia.The patient presented anorexia approximately 4 years ago, and the laboratory test results indicated hyponatremia. He was hospitalized 3 times subsequently due to past years, during which tests consistently severe hyponatremia.Upon admission, his serum osmolarity, urine sodium level, 24-hour level 277 mOsm/kg H2O, 465 82.5...
Appropriate immunohistochemistry (IHC) scoring criteria can guarantee the reliability of mutation detection results. Most current studies suggest that "four-grade criterion" may be best among all systems. The aim this study is to discuss influence different four-grade on test results.Three were respectively used evaluate EGFR status 83 cases non-small cell lung cancer (NSCLC) samples. sensitivity, specificity, positive predictive value (PPV), negative (NPV), agreement each criterion compared...
Patients with non-small cell lung cancer (NSCLC) harboring mutations of the epidermal growth factor receptor (EGFR) respond well to EGFR-tyrosine kinase inhibitor therapy. Immunohistochemistry (IHC) is a simple and widely used technique in clinical pathology laboratories. IHC also features cost effectiveness rapid detection EGFR compared molecular methods. This study aims determine accuracy for mutation NSCLC.Specimens (obtained from surgery or biopsy) 97 NSCLC cases were stained through...