The aim of this study was to examine the temporal sensitivity bovine embryos culture environment after fertilization determine which period, if any, is most critical in determining blastocyst quality. Bovine zygotes produced vitro were divided into six groups and cultured either (in synthetic oviductal fluid, SOF), vivo ewe oviduct) or a combination both systems. Development stage, ability blastocysts withstand cryopreservation relative abundance several gene transcripts examined. Culture...

Journal Article Susceptibility of Clostridium perfringens to C2–C18 fatty acids Get access E. Skřivanová, Skřivanová Research Institute Animal Production, Laboratory Physiology Nutrition and Products Quality, Prague, Czech Republic Search for other works by this author on: Oxford Academic Google Scholar M. Marounek, Marounek RepublicInstitute Genetics, Laboratories Developmental Biology, Academy Sciences, Dr Prague 10 – Uhříněves, CZ 104 01, (e‐mail: marounek@iapg.cas.cz). G. Dlouhá, Dlouhá...

Abstract Nucleolar ultrastructural changes occurring in vivo bovine oocytes during follicular growth were analyzed by electron microscopy. The rates of vitro incorporation 3 H‐uridine the same size class evaluated autoradiography. One to two large fibrillogranular, vacuolated nucleoli present from small medium antral follicles 0.5–3 mm diameter. These showed intense hnRNA and rRNA synthesis. homogeneous, agranular 3–4 diameter composed a compact fibrillar material. This morphological change...

We report numerical simulation and hyperspectral Raman imaging of three index-guiding solid-core photonic crystal fibers (PCFs) different air-cladding microstructures to assess their respective potential for evanescent-field spectroscopy, with an emphasis on achieving surface-enhanced scattering (SERS) over the entire fiber length. Suspended-core PCF consisting a silica core surrounded by large air channels conjoined thin web is most robust SERS-active PCFs, demonstrated detection...

We have shown previously that porcine cumulus and mural granulosa cells produce a factor is very similar, if not identical, to the oocyte-derived expansion-enabling (CEEF). Because growth differentiation 9 (GDF9) most likely candidate for CEEF, in present study we tested hypothesis GDF9 expressed only oocytes pig but also somatic follicular cells. In addition, asked whether relative abundance (RA) of mRNA changes and/or during periovulatory period or culture oocyte-cumulus complexes (OCCs)...

An unclad, multi-mode single crystal sapphire fiber was used as a platform, and immobilized colloidal Ag nanoparticles (NPs) were enabler, for evanescent-field fiber-optic sensing via surface-enhanced Raman scattering (SERS) of Rhodamine 6G (R6G) solution. The dependence the measured intensity on NP coverage density (to maximum 120 particles/μm²) well length 6 cm) investigated. We demonstrate utility SERS-active fibers sensitive measurements (10⁻⁸ M R6G). further reveal, with aid theoretical...

The progress of pronuclear DNA synthesis was monitored by the radioactive precursor 3H-thymidine during first cell cycle parthenogenetically activated bovine oocytes. Bovine oocytes were exposed to Ca2+ ionophore A23187 at 24, 30, or 36 h after onset in vitro maturation. Young 24-h subsequently cultured for 6 protein inhibitor, cycloheximide (CHX), ensure similar rates activation (96-100%) and formation (93-97%) among all groups Subsequent autoradiographic experiments revealed a slightly,...

Abstract MicroRNAs (miRNAs) are ubiquitous small RNAs guiding post-transcriptional gene repression in countless biological processes. However, the miRNA pathway mouse oocytes appears inactive and dispensable for development. We propose that marginalization of activity stems from constraints adaptations RNA metabolism elicited by diluting effects oocyte growth. report miRNAs do not accumulate like mRNAs during growth because turnover has adapted to it. The most abundant total tens thousands...

Changes in the level of transcriptional activity 32-cell stage morula nuclei were studied after blastomere electrofusion to enucleated oocytes. Nuclear transplant recipients pulse labelled with 3H-uridine during cultivation vitro, embryos then fixed and processed for autoradiography electron microscopy. Transcriptional substantially decreased 4.5 hr was completely inhibited at last 15 fusion. Transcription resumed thereafter two-cell could be detected both from 70% analyzed. rapidly...

We here report an innovative and scalable strategy of transforming a commercial unclad sapphire optical fiber to all-alumina nanostructured (NSOF). The entails coating with metal aluminum followed by anodization form alumina cladding highly organized pore channel structure. Through experiments numerical simulation, we demonstrate the utility benefit NSOF, analogous all-silica microstructured fiber, for evanescent-field surface-enhanced Raman scattering (SERS) measurements. experimentally...

Long-period gratings (LPGs) inscribed in endlessly single mode (ESM) photonic crystal fibers (PCFs) with symmetric and asymmetric CO2 laser irradiation are investigated both numerically experimentally. Parallel results from conventional (SMFs) presented for comparison. Theoretical predictions, transmission measurements, near-field imaging indicate that, regardless of the fiber type, index perturbation induced by aid a 120° gold-coated reflecting mirror LP(0n) coupling, while without using...

The mechanism of maternal protein degradation during preimplantation development has not been clarified yet. It is thought that a lot proteins are degraded by the ubiquitin–proteasome system. In this study, we focused on role SCF (Skp1-Cullin-F-box) complexes early bovine embryogenesis. We inhibited them using MLN4924, an inhibitor complex ligases controlled neddylation. Oocytes maturated in MLN4924 could be fertilized, but found no cumulus cell expansion and high number polyspermy after...

DNA, RNA and protein synthesis was studied in the epithelium of ductus epididymidis mouse by autoradiography.

Identification of genes that are important for normal preimplantation development is essential understanding the basics early mammalian embryogenesis. In our previous study, we have shown CENPF (mitosin) differentially expressed during bovine embryos. a centromere-kinetochore complex protein plays crucial role in cell division somatic cells. To best knowledge, no study has yet been done on either model, or oocytes and this focused fate embryos after injection double-stranded RNA (dsRNA) into...

Abstract Oocyte developmental competence is acquired during folliculogenesis and regulated by complex molecular mechanisms. Several molecules are involved in these mechanisms, including microRNAs (miRNAs) that essential for oocyte‐specific processes throughout the development. The objective of this study was to identify expression profile miRNAs porcine oocytes derived from follicles different sizes using RNA deep sequencing. Oocytes were aspirated large (LO; 3–6 mm) or small (SO; 1.5–1.9...

Summary. Pig oocytes were matured in vitro a modified M-199 medium for 44 h, subjected to electrical stimulation and scored activation 6 h later. Sham pulsed oocytes, exposed electroporation an a.c. field, did not develop the female pronucleus any more frequently than occurs spontaneously (8·3% within 50 of culture). However, single d.c. pulse proved extremely efficient activating pig oocytes. Pulses 0·75–1·65 kV cm−1 lasting 30 or 100 μs activated at least 90% The developmental pathway...