A5048935982- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Nicotinic Acetylcholine Receptors Study
- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
- Vestibular and auditory disorders
- Neurogenesis and neuroplasticity mechanisms
- Neural dynamics and brain function
- Lipid Membrane Structure and Behavior
- Receptor Mechanisms and Signaling
- Astrophysics and Cosmic Phenomena
- Particle physics theoretical and experimental studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurobiology and Insect Physiology Research
- RNA regulation and disease
- Heart Rate Variability and Autonomic Control
- Hearing, Cochlea, Tinnitus, Genetics
- Dark Matter and Cosmic Phenomena
- High-Energy Particle Collisions Research
- Neuroscience of respiration and sleep
- Quantum Chromodynamics and Particle Interactions
- Sleep and Wakefulness Research
- Tuberculosis Research and Epidemiology
- Mitochondrial Function and Pathology
- Anesthesia and Neurotoxicity Research
Takasaki University of Health and Welfare
2019-2025
Tokyo University of Agriculture and Technology
2024
National Institute of Information and Communications Technology
2024
Tohoku University
2021-2022
University of Alabama in Huntsville
2021
Waseda University
2008-2019
Gunma University
2000-2009
Gunma University Hospital
2008
Tokyo Medical and Dental University
2006
Japan Science and Technology Agency
1999-2005
1. N‐methyl‐D‐aspartate (NMDA)‐, quisqualate‐ and kainate‐induced currents were recorded in cultured rat hippocampal neurones using the whole‐cell voltage‐clamp technique. To isolate inward carried by Ca2+ other divalent cations (Sr2+, Ba2+, Mn2+ Mg2+), both Na+ K+ control external solution replaced with impermeant cation N‐methylglucamine (NMG). 2. Replacement of Na+, NMG abolished NMDA‐, kinate‐induced currents. In Na(+)‐, K(+)‐free (abbreviated simply as Na(+)‐free) containing 10 mM‐Ca2+...
Ionic mechanisms of excitation were studied in the immature egg cell membrane a starfish, Mediaster aequalis, by analyzing currents during voltage clamp. The shows two different inward current mechanisms. One is activated at potential -55 approximately -50 mV and other -7 -6 mV. They are referred to as channels I II, respectively. A similar difference also found half inactivation. Currents can, therefore, be separated selective both depend on Ca++ (Sr++ or Ba++) but only channel depends Na+....
We report on the highest energy photons from Crab Nebula observed by Tibet air shower array with underground water-Cherenkov-type muon detector array. Based criterion of a number measured in an shower, we successfully suppress 99.92% cosmic-ray background events energies E>100 TeV. As result, 24 photonlike TeV against 5.5 events, which corresponds to 5.6σ statistical significance. This is first detection astrophysical source.
Glial cells express a variety of neurotransmitter receptors. Notably, Bergmann glial in the cerebellum have Ca2+-permeable alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors (AMPARs) assembled without GluR2 subunit. To elucidate role these AMPARs, we converted them into Ca2+-impermeable by adenoviral-mediated delivery gene. This conversion retracted processes ensheathing synapses on Purkinje cell dendritic spines and retarded removal synaptically...
Evidence has been accumulated that glioblastoma cells release and exploit glutamate for proliferation migration by autocrine or paracrine loops through Ca 2+ -permeable AMPA-type receptors. Here, we show signaling mediated AMPA receptor regulates the growth motility of via activation Akt. supplied phosphorylated Akt at Ser-473, thereby facilitating mobility. A dominant-negative form inhibited cell accelerated overexpression receptor. In contrast, introduction a constitutively active rescued...
Effects of thyrotropin-releasing hormone (TRH; thyroliberin) on membrane electrical properties were studied in the clonal rat anterior pituitary cell (GH3) by continuous recording intracellular potential. Application TRH, which stimulates release prolactin and growth from GH3 cell, elicited a transient hyperpolarization followed an enhancement generation action potentials for extended period majority cells tested. The was due to increase conductance K+. spike not depolarization. input...
1 The presynaptic action of kainate (KA) receptor activation at the mossy fibre-CA3 synapse was examined using fluorescence measurement Ca2+ influx as well electrophysiological recordings in mouse hippocampal slices. 2 Bath application a low concentration (0·2 μM) KA reversibly increased amplitude volley evoked by stimulation fibres to 146 ± 6 % control (n= 6), whereas it reduced field excitatory postsynaptic potential (EPSPs) 30 4 %. 3 potentiating effect on volleys also observed Ca2+-free...
The four subunits of the AMPA-type glutamate receptor (GluR1–GluR4 or GluR-A–GluR-D) exist in two distinct forms, flip and flop, generated by alternative splicing a 115 bp region. GluR2 subunit plays key role determining functional properties AMPA channel. In this study, we examined differences kinetic between flop splice variants expressed Xenopus oocytes using fast agonist application techniques. Glutamate was applied to outside-out patches from with piezo-driven double-barreled pipettes....
The effect of a low concentration (1 μM) kainate (kainic acid; KA) on presynaptic calcium (Ca 2+ ) influx at the Schaffer collateral‐commissural (SCC) synapse was examined in rat hippocampal slices. Following selective loading terminals with fluorescent Ca indicator rhod‐2 AM, transient increases (pre[Ca ] t and field excitatory postsynaptic potentials (EPSPs) evoked by electrical stimulation SCC pathway were recorded simultaneously. Bath application 1 μM KA reversibly suppressed EPSPs...
1. Two different types of kainate response were recorded in cultured rat hippocampal neurons with the use whole-cell and outside-out configurations patch-clamp technique. 2. There was an outward rectification current-voltage (I-V) plot kainate-induced current (type I response) relatively large bearing a morphological resemblance to young pyramidal cells. In smaller elliptical somata fine neurites, characterized by remarkable inward I-V significant permeability Ca2+ II response). 3. Both type...
1. The effect of acetylcholine (ACh) on the rat adrenal chromaffin cell membrane was examined in culture by using an intracellular recording technique. ACh applied with gas pressure through a pipette which had internal tip diameter about 3 μm. 2. Upon application depolarized superimposed potential fluctuations. Occasionally repetitive spikes were observed during entire period up to 30 sec. In some cells hyperpolarization slow time course followed initial depolarization. 3. depolarization...
The differential cross sections of the Σ^{-}p→Λn reaction were measured accurately for Σ^{-} momentum (p_{Σ}) ranging from 470 to 650 MeV/c at J-PARC Hadron Experimental Facility. Precise angular information about was obtained first time by detecting approximately 100 events each step Δcosθ=0.1. show a slightly forward-peaking structure in regions. integrated -0.7≤cosθ≤1.0 as 22.5±0.68 [statistical error(stat.)] ±0.65 [systematic error(syst.)] mb and 15.8±0.83(stat)±0.52(syst)...
1. The effect of synthetic joro spider toxin (JSTX‐3) on alpha‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid (AMPA) receptor channels in cultured rat hippocampal neurones was investigated using the whole‐cell patch‐clamp technique. 2. A population had AMPA receptors with strong inward rectification and substantial Ca2+ permeability (type II neurones), whereas most I neurones) slight outward little permeability. JSTX‐3 selectively suppressed inwardly rectifying Ca(2+)‐permeable expressed...
1 To investigate mechanisms responsible for the presynaptic inhibitory action mediated by axonal group II metabotropic glutamate receptor (mGluR) at mossy fibre-CA3 synapse, we used a quantitative fluorescence measurement of Ca2+ in mouse hippocampal slices. 2 Bath application mGluR-specific agonist (2S,1′R,2′R,3′R)-2-(2,3-dicarboxycyclopropyl)glycine (DCG-IV, μM) reversibly suppressed influx (to 55·2 ± 4·6 % control, n= 5) as well field EPSPs recorded simultaneously 3·1 2·0 %). Presynaptic...
Glutamate transporters are essential for terminating excitatory neurotransmission. Two distinct glutamate transporters, glutamate–aspartate transporter (GLAST) and amino acid 4 (EAAT4), expressed most abundantly in the molecular layer of cerebellar cortex. GLAST is Bergmann glial processes surrounding synapses on Purkinje cell dendritic spines, whereas EAAT4 concentrated extrasynaptic regions spine membranes. To clarify functional significance coexistence these we analyzed kinetics EPSCs...