A5019524161- Radiation Therapy and Dosimetry
- Spaceflight effects on biology
- Effects of Radiation Exposure
- Neuroscience and Neuropharmacology Research
- DNA Repair Mechanisms
- Glioma Diagnosis and Treatment
- Advanced Radiotherapy Techniques
- Mitochondrial Function and Pathology
- Neurogenesis and neuroplasticity mechanisms
- Cell death mechanisms and regulation
- Advanced X-ray and CT Imaging
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer-related Molecular Pathways
- Cancer, Hypoxia, and Metabolism
- Anesthesia and Neurotoxicity Research
- Radiation Detection and Scintillator Technologies
- Heat shock proteins research
- Space Exploration and Technology
- Ion channel regulation and function
- Medical Imaging Techniques and Applications
- RNA Interference and Gene Delivery
- Advanced Semiconductor Detectors and Materials
- Boron Compounds in Chemistry
- MicroRNA in disease regulation
- Blood groups and transfusion
Gunma University Hospital
2015-2024
Gunma University
2008-2024
Kansai Medical University
2019-2023
Anjo Kosei Hospital
2012
Niigata University
2008
Kobe Gakuin University
2002-2004
Osaka University
2003
Toranomon Hospital
2000
Fukuoka Hospital
1992
Evidence has been accumulated that glioblastoma cells release and exploit glutamate for proliferation migration by autocrine or paracrine loops through Ca 2+ -permeable AMPA-type receptors. Here, we show signaling mediated AMPA receptor regulates the growth motility of via activation Akt. supplied phosphorylated Akt at Ser-473, thereby facilitating mobility. A dominant-negative form inhibited cell accelerated overexpression receptor. In contrast, introduction a constitutively active rescued...
Carbon ion radiotherapy (C-ion RT) offers superior dose conformity in the treatment of deep-seated tumors compared with conventional X-ray therapy. In addition, carbon beams have a higher relative biological effectiveness protons or beams. C-ion RT for first patient at Gunma University Heavy Ion Medical Center (GHMC) was initiated March 2010. The major specifications facility were determined based on experience clinical treatments National Institute Radiological Sciences (NIRS), size and...
DNA double-strand breaks (DSBs) induced by ionizing radiation pose a major threat to cell survival. The can respond the presence of DSBs through two repair pathways: homologous recombination (HR) and nonhomologous end joining (NHEJ). Higher levels death are high-linear energy transfer (LET) when compared low-LET radiation, even at same physical doses, due less effective efficient repair. To clarify whether high-LET inhibits all pathways or specifically one pathway, studies were designed...
pAkt/Glioblastoma/Radiation therapy.phosphorylated-Akt (pAkt) plays an important role in tumorigenesis through promotion of cell survival by inhibiting apoptosis and mediating proliferation.Higher expression pAkt has been reported to be associated with unfavorable prognosis several malignant tumors.In this study, the prognostic value was investigated glioblastomas using immunohistochemical methods.Tissue sections obtained from 64 patients glioblastoma were evaluated.The mean median follow-up...
Background and Purpose To understand the mechanisms involved in strong killing effect of carbon-ion beam irradiation on cancer cells with TP53 tumor suppressor gene deficiencies. Materials Methods DNA damage responses after or X-ray isogenic HCT116 colorectal cell lines without (p53+/+ p53-/-, respectively) were analyzed as follows: survival by clonogenic assay, death modes morphologic observation DAPI-stained nuclei, double-strand breaks (DSBs) immunostaining phosphorylated H2AX (γH2AX),...
Our previous data demonstrated that targeting non-homologous end-joining repair (NHEJR) yields a higher radiosensitivity than homologous recombination (HRR) to heavy ions using DNA gene knockouts (KO) in mouse embryonic fibroblast (MEF). In this study, we determined if combining the use of an NHEJR inhibitor with carbon (C) ion irradiation was more efficient killing human cancer cells compared only HRR inhibitor. The TP53-null non-small cell lung line H1299 used for testing radiosensitizing...
Abstract In cancer therapy today, carbon ion radiotherapy is used mainly as monotherapy, whereas cisplatin concomitantly with X-ray radiotherapy. The effectiveness of concomitant ions and unclear. To obtain the information on mechanisms potentially shared between or X-rays cisplatin, we assessed correlation sensitivity to single treatments. 20 human cell lines, strongly correlated indicating presence target mechanisms. Interestingly, was much weaker than that different cisplatin. Assessment...
Space radiation and microgravity (μ
This study aimed to establish a setup for ultra-high-dose-rate (FLASH) carbon-ion irradiation, and conduct the first human cell experiments using FLASH carbon ions.A system irradiation (1-3 Gy at 13 or 50 keV/μm) was developed. The growth senescence of HFL1 lung fibroblasts were assessed by crystal violet staining assays senescence-associated β-galactosidase staining, respectively. Survival HSGc-C5 cancer cells clonogenic assays.The dose rates ions ranged from 96-195 Gy/s, meeting definition...
Abstract Hippocampal CA3 pyramidal neurons receive synaptic inputs from both mossy fibres (MFs) and associational (AFs). Long‐term potentiation (LTP) at these synapses differs in its induction sites N ‐methyl‐ D ‐aspartate receptor (NMDAR) dependence. Most evidence favours the presynaptic postsynaptic mechanisms for of MF LTP AF LTP, respectively. This implies that molecular functional properties differ between sites. In this study, we focused on difference trafficking...
The present study investigated the ability of carboplatin and paclitaxel to sensitize human non-small-cell lung cancer (NSCLC) cells carbon-ion beam irradiation. NSCLC H460 treated with or were irradiated X-rays beams, radiosensitivity was evaluated by clonogenic survival assay. Cell proliferation determined counting number viable using Trypan blue. Apoptosis senescence terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) staining senescence-associated...
Abstract Carbon-ion radiotherapy (CIRT) holds promise to treat inoperable locally-advanced non-small cell lung carcinoma (NSCLC), a disease poorly controlled by standard chemoradiotherapy using X-rays. Since CIRT is an extremely limited medical resource, selection of NSCLC patients likely benefit from it important; however, biological predictors response are ill-defined. The present study investigated the association between mutational status EGFR and KRAS , driver genes frequently mutated...
Multiple unique environmental factors such as space radiation and microgravity (μG) pose a serious threat to human gene stability during travel. Recently, we reported that simultaneous exposure of fibroblasts simulated μG results in more chromosomal aberrations than cells exposed alone. However, the mechanisms behind this remain unknown. The purpose study was thus obtain comprehensive data on expression using three-dimensional clinostat synchronized carbon (C)-ion or X-ray irradiation...
The epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (E-TKI) resistance has emerged as an important clinical issue. To overcome this resistance, researchers have examined different modalities, either for use a monotherapy or in combination with E-TKI therapy. In the present study, we investigated whether decrease nitric oxide (NO) levels affects radiosensitization of non-small cell lung cancer (NSCLC) lines. A549 and H3255 NSCLC cells were examined. They subjected to hypoxic...
Purpose To develop a silicon (Si) and cadmium telluride (CdTe) imaging Compton camera for biomedical application on the basis of technologies used astrophysical observation to test its capacity perform three-dimensional (3D) imaging. Materials Methods All animal experiments were performed according Animal Care Experimentation Committee (Gunma University, Maebashi, Japan). Flourine 18 fluorodeoxyglucose (FDG), iodine 131 (131I) methylnorcholestenol, gallium 67 (67Ga) citrate, separately...
Neurons are essential components of neural circuits and provide brain function organization. We previously reported that X irradiation induces apoptosis in immature neurons. To the best our knowledge, there have been few reports investigating effects on mature analyzed morphology, density cytoskeletal proteins dendritic spines prepared developing hippocampal neurons from 18 days embryo by using Banker's method. at 21 vitro were irradiated several doses immediately fixed. evaluate spine...
This study aimed to analyze whether Ulinastatin, a urinary trypsin inhibitor (UTI), inhibits the TGF-β signaling pathway and lung fibrosis induced by thoracic irradiation in injury mouse model. The thoraces of 9-week-old female fibrosis-sensitive C57BL/6 mice were irradiated with single X-ray dose 12 Gy or 24 Gy. UTI was administrated intraperitoneally at 200,000 units/kg concurrently radiation (concurrent UTI) daily during post-irradiation period for 8–14 days (post-RT UTI). Mice sacrificed...