A5006602926- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Esophageal Cancer Research and Treatment
- Catalytic Cross-Coupling Reactions
- Catalytic C–H Functionalization Methods
- Endometrial and Cervical Cancer Treatments
- CAR-T cell therapy research
- Microbial Natural Products and Biosynthesis
- Synthesis and Catalytic Reactions
- ATP Synthase and ATPases Research
- Cancer, Stress, Anesthesia, and Immune Response
- Marine Sponges and Natural Products
- Cancer Research and Treatments
- Immune cells in cancer
- Cervical Cancer and HPV Research
- Cancer-related Molecular Pathways
- Asymmetric Hydrogenation and Catalysis
- Advanced Glycation End Products research
- Immune Response and Inflammation
- Glioma Diagnosis and Treatment
- Synthetic Organic Chemistry Methods
- Catalytic Alkyne Reactions
- Nanoplatforms for cancer theranostics
- Management of metastatic bone disease
Nagoya Ekisaikai Hospital
2025
Fukushima Medical University
2020-2024
Sumitomo Chemical (Japan)
2019-2023
Kyoto University
2008-2023
Gunma University
2014-2023
Hidaka Hospital
2023
Kyoto Katsura Hospital
2023
Takara (Japan)
2019-2021
Sungkyunkwan University
2021
Karolinska Institutet
2014-2018
Accumulating evidence suggests that exogenous cellular stress induces PD-L1 upregulation in cancer. A DNA double-strand break (DSB) is the most critical type of genotoxic stress, but involvement DSB repair expression has not been investigated. Here we show cancer cells upregulated response to DSBs. This requires ATM/ATR/Chk1 kinases. Using an siRNA library targeting genes, discover BRCA2 depletion enhances Chk1-dependent after X-rays or PARP inhibition. In addition, Ku70/80 substantially...
Although it has been shown that chemoradiotherapy may induce immunogenic cell death, which could trigger T-cell immunity mediated by high-mobility group box 1 protein (HMGB1) and calreticulin, there is still limited information to support this theory directly in a clinical setting. In the present study, we evaluated antigen-specific responses against six cancer-testis antigens peripheral blood lymphocytes from patients with esophageal squamous carcinoma (ESCC) receiving chemoradiation....
Sterisch abgeschirmt: Der Eisen-Phosphan-Komplex 1 vermittelt eine hoch C-selektive Alkinylierung nichtaktivierter Alkylhalogenide mit entsprechenden Grignard-Reagentien. Primäre und sekundäre Alkyliodide, -bromide -chloride liefern in guten Ausbeuten die substituierten Alkine, anschließend Cyclisierungen Kreuzkupplungen eingehen können. Detailed facts of importance to specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset....
// Yago Pico de Coaña 1 , Maria Wolodarski 1, 2 Isabel Poschke 3 Yuya Yoshimoto 4 Yuan Yang 5 Nyström Ulrika Edbäck Suzanne Eghyazi Brage Andreas Lundqvist 6 Giuseppe V. Masucci Johan Hansson Rolf Kiessling Department of Oncology and Pathology, Cancer Center Karolinska, Karolinska Institutet, Stockholm, Sweden University Hospital Solna, Division Molecular Gastrointestinal Tumors, German Research Center, Heidelberg, Germany Radiation Oncology, Gunma Graduate School Medicine, Maebashi, Gunma,...
Purpose There is growing evidence that tumor-specific immune responses play an important role in anti-cancer therapy, including radiotherapy. Using mouse tumor models we demonstrate irradiation-induced anti-tumor immunity essential for the therapeutic efficacy of irradiation and can be augmented by modulation cytotoxic T lymphocyte (CTL) activity. Methods Materials C57BL/6 mice, syngeneic EL4 lymphoma cells, Lewis lung carcinoma (LL/C) cells were used. Cells injected into right femurs mice....
In the presence of N,N,N′,N′-tetramethylethylenediamine (TMEDA) as an additive, easily prepared and handled N-chloroamines react with aryl Grignard reagents to give a variety arylamines in good excellent yields. Functional groups such ester nitrile are compatible under reaction conditions.
In the presence of 0.5−1 mol % FeCl3 with lithium bromide as a crucial additive, alkynyl Grignard reagents, prepared from corresponding alkynes and methylmagnesium bromide, react alkenyl bromides or triflates to give conjugated enynes in high excellent yields. The reaction shows wide applicability various terminal electrophiles.
Novel iron-catalyzed amination reactions of various aryl bromides have been developed for the synthesis diaryl- and triarylamines. The key to success this protocol is use in situ generated magnesium amides presence a lithium halide, which dramatically increases product yield. present method simple free precious expensive metals ligands, thus providing facile route triarylamines, recurrent core unit organic electronic materials as well pharmaceuticals.
X-ray radiotherapy activates tumor antigen-specific T-cell responses, and increases in the serum levels of high mobility group box 1 (HMGB1) induced by irradiation play a pivotal role activating anti-tumor immunity. Here, we examined whether carbon-ion beams, as well X-rays, can induce HMGB1 release from human cancer cell lines. The study five lines: TE2, KYSE70, A549, NCI-H460 WiDr. proportion cells surviving X- or beam was assessed clonogenic assay. D 10 , dose at which 10% survive,...
Anaplastic thyroid carcinoma (ATC) is one of the most aggressive forms cancer with no curative therapies available. To date, strategies to target ATC by immunotherapy have not been evaluated. We investigated whether would be a suitable for natural killer (NK) cell-based immunotherapy.We first established seven new cell lines from tumors, three papillary tumors and analyzed them together eight additional lines. Cells were sensitivity lysis NK cells their ability chemoattract regulate activity...
To develop more effective therapies for patients with advanced gastric cancer, we examined the potential of ex vivo expanded natural killer (NK) cells. We assessed expression ligands NK Group 2 Member D (NKG2D, an important activation molecule) in primary tumors from 102 cancer by immunohistochemistry and determined their prognostic value. then vitro cytotoxicity cells healthy donors cancer. The resting interleukin (IL)‐2‐activated was compared to that 7 days coculture K562‐mb15‐4.1BBL cell...
Adoptive cell therapy (ACT) is becoming a prominent alternative therapeutic treatment for cancer patients relapsing on traditional therapies. In parallel, antibodies targeting immune checkpoint molecules, such as cytotoxic-T-lymphocyte-associated antigen 4 (CTLA-4) and death protein 1 pathway (PD-1), are rapidly being approved multiple types, including first line PD-L1-expressing non-small-cell lung cancer. The combination of ACT blockade could substantially boost the efficacy ACT. this...
Anti-tumor immunity modulates the local effects of radiation therapy. High mobility group box 1 (HMGB1) plays a pivotal role in activating antigen-specific T-cell responses. Here, we examined relationship between linear energy transfer (LET) and HMGB1 release. We assessed proportions KYSE-70, HeLa SiHa cells surviving after carbon-ion (C-ion) beam irradiation with different LET values, using clonogenic assay. The D10, dose at which 10% survived, was calculated linear–quadratic model. levels...
Background Tumor-associated antigen (TAA)-specific CD8(+) T cells are essential for nivolumab therapy, and irradiation has been reported to have the potential generate activate TAA-specific cells. However, mechanistic insights of T-cell response during combinatorial immunotherapy using radiotherapy still largely unknown. Methods Twenty patients included in this study were registered CIRCUIT trial (ClinicalTrials.gov, NCT03453164 ). All had multiple distant metastases intolerance or...
Abstract In cancer therapy today, carbon ion radiotherapy is used mainly as monotherapy, whereas cisplatin concomitantly with X-ray radiotherapy. The effectiveness of concomitant ions and unclear. To obtain the information on mechanisms potentially shared between or X-rays cisplatin, we assessed correlation sensitivity to single treatments. 20 human cell lines, strongly correlated indicating presence target mechanisms. Interestingly, was much weaker than that different cisplatin. Assessment...
Quinone outside inhibitors (QoIs) are one of the major agricultural fungicide groups used worldwide. However, development resistance by different pathogenic species associated with specific mutation at target gene site is becoming a critical issue for sustainable use QoIs. The authors aimed to design novel QoI molecule overcome aforementioned issue. A rational approach avoid steric hindrance between and mutated was successfully employed. resulting compound, metyltetraprole, characterized...
The current study aimed to evaluate the outcomes of patients with adenocarcinoma (AC) uterine cervix after definitive radiotherapy (RT) and prognostic factors, including immunity-related molecules. A total 71 AC from multiple Japanese institutions were retrospectively analysed. Histological subtypes diagnosed according 2014 World Health Organization classification. All underwent RT comprising external beam intracavitary brachytherapy or without concurrent chemotherapy. Immunohistochemical...
<title>Abstract</title> <bold>Introduction</bold> Multiple surgical options for the treatment of metacarpal fractures are available, including cross-pinning, intramedullary Kirschner wires or screws, and plate screw fixation. However, despite fixation being biomechanically superior to other methods, clinical cases correction loss displacement continue occur. This study aimed elucidate incidence complications outcomes fractures. <bold>Materials Methods</bold> We examined 334 osteosynthesis...
Enantioselective total synthesis of anhydrophytosphingosine pachastrissamine (jaspin B) was achieved using Sharpless asymmetric dihydroxylation and dirhodium(II)-catalyzed C-H amination as key steps.
Objective Enhancing immunologic responses, including human leukocyte antigen (HLA) class I expression on tumor cells and recognition elimination of by tumor-specific cytotoxic T lymphocyte (CTL), is considered a novel concept radiotherapy. The present study examined patients who underwent preoperative hyperthermo-chemoradiotherapy (HCRT) for locally advanced rectal cancer to assess the correlation between HLA clinical outcome. Materials Methods Seventy-eight with adenocarcinoma received HCRT...
ObjectiveTo elucidate tumor mutation profiles associated with outcomes of uterine cervical cancer (UCC) patients treated definitive radiotherapy.MethodsNinety-eight newly diagnosed and pathologically confirmed UCC (82 squamous cell carcinomas, 12 adenocarcinomas, four adenosquamous carcinomas) who were radiotherapy analyzed. DNA was extracted from pre-treatment biopsy specimens. The exons 409 cancer-related genes sequenced using a next-generation sequencer. Genetic mutations identified...
Radiotherapy induces an immune response in the cancer microenvironment that may influence clinical outcome. The present study aimed to analyse alteration of CD8<sup>+</sup> T‑cell infiltration and programmed death‑ligand 1 (PD‑L1) expression following radiotherapy samples from patients with uterine cervical squamous cell carcinoma. Additionally, current sought association between these responses outcomes. A total 75 who received either definitive chemoradiotherapy or were retrospectively...