A5038494133- Atomic and Subatomic Physics Research
- Advanced NMR Techniques and Applications
- Advanced MRI Techniques and Applications
- Electron Spin Resonance Studies
- Lanthanide and Transition Metal Complexes
- Quantum, superfluid, helium dynamics
- Biochemical effects in animals
- Nanoplatforms for cancer theranostics
- Head and Neck Cancer Studies
- MRI in cancer diagnosis
- Mechanical and Optical Resonators
- Medical Imaging Techniques and Applications
- Supramolecular Chemistry and Complexes
- NMR spectroscopy and applications
- Analytical Chemistry and Sensors
- Body Composition Measurement Techniques
- Free Radicals and Antioxidants
- Cardiovascular and exercise physiology
- Nanopore and Nanochannel Transport Studies
- Magnetism in coordination complexes
- Cephalopods and Marine Biology
- Marine and coastal plant biology
- Cancer Genomics and Diagnostics
- Cervical Cancer and HPV Research
- Ultrasound Imaging and Elastography
DKFZ-ZMBH Alliance
2003-2025
German Cancer Research Center
2004-2025
Heidelberg University
2007-2025
Charité - Universitätsmedizin Berlin
2022-2025
Karlsruhe Institute of Technology
2024
Leibniz-Forschungsinstitut für Molekulare Pharmakologie
2014-2023
Humboldt-Universität zu Berlin
2022
Freie Universität Berlin
2022
Forschungsverbund Berlin
2020
Universitätskinderklinik
2019
A magnetic resonance approach is presented that enables high-sensitivity, high-contrast molecular imaging by exploiting xenon biosensors. These sensors link atoms to specific biomolecular targets, coupling the high sensitivity of hyperpolarized nuclei with specificity biochemical interactions. We demonstrated spatial resolution a target protein in vitro at micromolar concentration, readout scheme reduces required acquisition time >3300-fold relative direct detection. This technique uses...
Magnetic resonance imaging (MRI) is seriously limited when aiming for visualization of targeted contrast agents. Images are reconstructed from the weak diamagnetic properties sample and require an abundant molecule like water as reporter. Micromolar to millimolar concentrations conventional agents needed generate image contrast, thus excluding many molecular markers potential targets. To address this limitation, we developed characterized a functional xenon NMR biosensor that can identify...
Cancer-related cachexia is an obscure syndrome leading to muscle wasting, reduced physical fitness and quality of life. The aim this study was assess morphology, metabolism, microcirculation in skeletal muscles patients with cancer-related compare these data matched healthy volunteers.In 19 cancer-induced age-, gender-, body-height-matched volunteers body composition aerobic capacity (VO(2max)) were analyzed. Skeletal fiber size capillarization evaluated biopsies the vastus lateralis muscle....
Abstract The targeting of metabolically labeled glycans with conventional MRI contrast agents has proved elusive. In this work, which further expands the utility xenon Hyper‐CEST biosensors in cell experiments, we present first successful molecular imaging such using MRI. Xenon are a novel class very high sensitivity. We designed multimodal biosensor for both fluorescent and detection that is targeted to sialic acid through bioorthogonal chemistry. Through use state art live‐cell bioreactor,...
Labile complexation of cucurbit[6]uril with xenon becomes detectable through saturation transfer highly efficient gas turnover for ultra-sensitive NMR applications.
Abstract Caged xenon has great potential in overcoming sensitivity limitations for solution‐state NMR detection of dilute molecules. However, no application such a system as magnetic resonance imaging (MRI) contrast agent yet been performed with live cells. We demonstrate MRI localization cells labeled caged packed‐bed bioreactor working under perfusion hyperpolarized‐xenon‐saturated medium. Xenon hosts enable NMR/MRI experiments switchable and selectivity cell‐associated versus unbound...
Abstract Reversibly bound Xe is a sensitive NMR and MRI reporter with its resonance frequency being influenced by the chemical environment of host. Molecular imaging enzyme activity presents promising approach for disease identification, but current biosensing concepts are limited since substrate conversion typically has little impact on shift inside tailored cavities. Herein, we exploit ability product enzymatic reaction to bind itself macrocyclic hosts CB6 CB7 thereby displace Xe. We...
We demonstrate a concept for multichannel MRI cell-labeling using encapsulated laser-polarized xenon. Conceptually different Xe trapping properties of two nanocarriers, namely macrocyclic cages as individual hosts or compartmentalization into nanodroplets, ensure large chemical shift separation bound in either the carriers even after cellular internalization. Two differently labeled mammalian cell populations were imaged by frequency selective saturation transfer resulting switchable...
The reversible binding of xenon to host molecules has found numerous applications in nuclear magnetic resonance studies. Quantitative characterization the Xe exchange dynamics is important understand and optimize physico-chemical behavior such hosts, but often challenging achieve at low concentrations. We have investigated a sensitive quantification technique based on chemical saturation transfer with hyperpolarized nuclei, qHyper-CEST. Using simulated signals we demonstrated that...
Guest capture and release are important properties of self-assembling nanostructures. Over time, a significant fraction guests might engage in short-lived states with different symmetry stereoselectivity transit frequently between multiple environments, thereby escaping common spectroscopy techniques. Here, we investigate the cavity an iron-based metal organic polyhedron (Fe-MOP) using spin-hyperpolarized
Abstract Fluorescent derivatives of the 129 Xe NMR contrast agent cryptophane‐A were obtained by functionalization with near infrared fluorescent dyes DY680 and DY682. The resulting conjugates spectrally characterized, their interaction giant large unilamellar vesicles varying phospholipid composition was analyzed fluorescence spectroscopy. In latter, a chemical exchange saturation transfer hyperpolarized (Hyper‐CEST) used to obtain sufficient sensitivity. To determine partitioning...
Ultrasound and hyperpolarized magnetic resonance imaging enable the visualization of biological processes in deep tissues. However, few molecular contrast agents are available to connect these modalities specific aspects function. We recently discovered that a unique class gas‐filled protein nanostructures known as gas vesicles could serve nanoscale reporters for modalities. need produce via expression specialized cultures cyanobacteria or haloarchaea limits their broader adoption by other...
Xenon is well known to undergo host-guest interactions with proteins and synthetic molecules. As xenon can also be hyperpolarized by spin exchange optical pumping, allowing the investigation of highly dilute systems, it makes an ideal nuclear magnetic resonance probe for such host The utility as a further improved using Chemical Exchange Saturation Transfer nuclei (Hyper-CEST), but accurate experiments requires polarizer infusion system optimized measurements. We present design...
A nanoparticulate carrier system is used to efficiently deliver a contrast agent for highly sensitive xenon Hyper-CEST MRI. The not only improves the biocompatibility and solubility of agent, it also allows selective cell targeting as demonstrated by discrimination human brain capillary aortic endothelial cells. As service our authors readers, this journal provides supporting information supplied authors. Such materials are peer reviewed may be re-organized online delivery, but copy-edited...
Research on molluscan specimens is increasingly being carried out using high-throughput molecular techniques. Due to their efficiency, these technologies have effectively resulted in a strong bias towards genotypic analyses. Therefore, the future large-scale correlation of such data with phenotype will require significant increase output morphological studies. Three-dimensional (3D) scanning techniques as magnetic resonance imaging (MRI) or computed tomography (CT) can achieve this goal they...
Trapping xenon in functionalized cryptophane cages makes the sensitivity of hyperpolarized (HP) $^{129}\mathrm{Xe}$ available for specific NMR detection biomolecules. Here, we study signal transfer onto a reservoir unbound HP by gating residence time nuclei cage through temperature-dependant exchange rate. Temperature changes larger than $\ensuremath{\sim}0.6\text{ }\text{ }\mathrm{K}$ are detectable as an altered signal. The temperature response is adjustable with lower concentrations caged...
Abstract A new approach to MRI thermometry using encapsulated hyperpolarized xenon is demonstrated. The method based on the temperature dependent chemical shift of in a cryptophane‐A cage. This linear with slope 0.29 ppm °C −1 which perceptibly higher than proton resonance frequency water (ca. 0.01 ) that currently used for thermometry. Using spectroscopic imaging techniques, we collected maps phantom sample could discriminate by direct NMR detection between differences 0.1 at sensor...
The NMR signal of hyperpolarized 129Xe trapped in cryptophane cages different solvents experiences chemical shifts. An encoding method is presented that involves the optimal use reversible Xe binding and efficiently uses hyperpolarization. This utilized nanomolar imaging, subsecond time-resolved studies while maintaining high spectral selectivity. Detailed facts importance to specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or...
The combination of hyperpolarized Xe with chemical exchange saturation transfer (Hyper-CEST) is a powerful NMR technique to detect highly dilute concentrations binding sites using RF pulses. Crucially, that pulse strength and duration generates the maximal Hyper-CEST effect priori unknown. In contrast CEST in proton MRI, where system reaches steady-state for long times, has an optimal time, i.e. saturating shorter or longer reduces effect. Here, we derive expressions this length. We also...