A5024070604- Pituitary Gland Disorders and Treatments
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Liver Disease Diagnosis and Treatment
- Neuroblastoma Research and Treatments
- Reproductive Biology and Fertility
- Cancer, Lipids, and Metabolism
- Receptor Mechanisms and Signaling
- Growth Hormone and Insulin-like Growth Factors
- Neuropeptides and Animal Physiology
- Ion Transport and Channel Regulation
- RNA modifications and cancer
- Adenosine and Purinergic Signaling
- TGF-β signaling in diseases
- Parvovirus B19 Infection Studies
- Circular RNAs in diseases
- Sarcoma Diagnosis and Treatment
- Renal and related cancers
- Cancer-related gene regulation
- Gout, Hyperuricemia, Uric Acid
- Neuroendocrine Tumor Research Advances
- Cancer-related molecular mechanisms research
- Gastric Cancer Management and Outcomes
- Histone Deacetylase Inhibitors Research
- Phagocytosis and Immune Regulation
Peking University People's Hospital
2017-2025
Peking University
2017-2025
Hebei Academy of Sciences
2025
Hebei University of Chinese Medicine
2021
Shandong Academy of Chinese Medicine
2021
Augusta University
2017
Georgia Regents Medical Center
2017
Baylor College of Medicine
2017
Cedars-Sinai Medical Center
1994-2015
Huizhou Central People's Hospital
2015
Abstract Pathogenesis of tumor formation in the anterior pituitary has been intensively studied, but common mechanism involved cell transformation and tumorigenesis remains elusive. In this study, we used mRNA differential display PCR to identify mRNAs that are differentially expressed rat cells compared with normal tissue. An exclusively not was characterized. Using tumor-specific product as a probe screen cDNA library constructed from GH4 cells, 974 bp isolated. This encodes novel protein...
Molecular mechanisms of pituitary tumorigenesis were studied using Polymerase chain reaction-single stranded conformational polymorphism with DNA sequencing to identify potential mutations in the ras protooncogenes and tumor suppressor gene p53 invasive adenomas carcinomas. Sequencing exons 5 through 8 revealed no mutations, nor detected N- or K-ras four carcinomas their respective metastatic deposits. Point H-ras however, identified three distant secondaries, but not primary carcinomas, six...
TASK3 gene (Kcnk9) is amplified and overexpressed in several types of human carcinomas. In this report, we demonstrate that a point mutation (G95E) within the consensus K+ filter not only abolished potassium channel activity but also abrogated its oncogenic functions, including proliferation low serum, resistance to apoptosis, promotion tumor growth. Furthermore, provide evidence TASK3G95E dominant-negative mutation, because coexpression wild-type mutant resulted inhibition current...
Pituitary tumor-transforming gene (PTTG) encodes a protein implicated in cellular transformation and transcriptional regulation. To identify downstream target genes, I established cell lines with tightly regulated inducible expression of PTTG. DNA arrays were used to analyze profiles after PTTG induction. identified c-myc oncogene as major PTTGtarget. Induction resulted increased proliferation through activation c-myc. showed that activates transcription transfected cells. binds promoter...
Pituitary tumor-transforming gene (PTTG) is a recently characterized oncogene whose expression product contains transcriptional activation domain at the C terminus. To understand mechanisms involved in PTTG biological functions, we used yeast two-hybrid screening to identify proteins that interact with PTTG. This study reports isolation and characterization of novel PTTG-binding factor (PBF). PBF an open reading frame 179 amino acids predicted molecular mass 22 kDa. In Northern blot...
Inhibin gene expression in the ovary is stimulated by FSH, which uses cAMP as an intracellular second messenger. To examine further transcriptional regulation of α inhibin FSH and cAMP, we have isolated characterized a genomic clone that contains entire rat gene. Sequence analysis promoter region revealed several potential response elements (CREs) transcription factor AP2-binding sites might mediate regulation. determine functional importance these sequences, fusion genes including 5′...
The echinoderm microtubule-associated protein-like 4-anaplastic lymphoma kinase (EML4-ALK) fusion gene resulting from an inversion within chromosome 2p occurs in approximately 5% of non-small cell lung cancer and is mutually exclusive with Ras EGFR mutations. In this study, we have used a potent selective ALK small molecule inhibitor, NPV-TAE684, to assess the oncogenic role EML4-ALK (NSCLC). We show here that TAE684 inhibits proliferation induces cycle arrest, apoptosis, tumor regression...
Silicosis is an occupational disease characterized as inflammatory cells infiltration and severe progressive pulmonary fibrosis. Kaempferol (Kae), a flavonoid that exists in many plants fruits, has been proved to have anti-inflammatory antifibrosis functions. However, the effects of Kae on silicosis remain unclear. In present study, we analyzed therapeutic 1-, 7-, 28-day models, respectively. 1-day model, treatment did not vary wet-to-dry weight ratios lung, apoptotic rate, autophagy, or...
Hepatitis B virus (HBV)-derived hepatocellular cancer (HCC) is a serious threat to human health, especially in China. There no highly sensitive and specific HCC biomarker at present, which makes it difficult detect the early stage. Serum exosomal circular RNAs (circRNAs) have been reported as novel diagnostic prognostic biomarkers of cancers. In present study, we aimed explore performance serum circRNAs for HBV-derived screening. At first, many were found be differentially expressed exosomes...
Pituitary tumor-transforming gene (PTTG) is a recently characterized oncogene that can act as transcriptional activator. In this study, we have the transactivation domain of PTTG. Transient transfection fusion constructs containing GAL4 DNA-binding and different parts PTTG indicated located between amino acids 119 164. Mitogen-activated protein (MAP) kinase cascade important in regulation cell growth, apoptosis, differentiation. Therefore, explored possibility plays role regulating function....
Gastrointestinal malignancy is a severe public health threat worldwide, and survival for most types of gastrointestinal cancer very poor. Therefore, finding better biomarkers to diagnose predict patient essential. HDAC1 has been reported be closely associated with several cancer, but the precise role in not clear. Recently, quite few studies have investigated correlation between expression clinical features or prognosis multiple malignancies, results were inconsistent. In this study, we...
Inhibin is an important modulator of reproductive function at both the endocrine level, through its regulation pituitary FSH biosynthesis, and paracrine autocrine levels, as intragonadal regulatory factor. To investigate in vivo actions inhibin gonadal function, transgenic mice that overexpress rat α-subunit gene were generated. A transgene includes mouse metallothionein-I promoter (MT-α) fused to precursor coding sequences was used produce three lines mice. Transgene mRNA expressed numerous...
Abstract Objectives Circular RNAs (circRNAs) are known to be associated with cardiovascular diseases. At present, an ideal biomarker for the early diagnosis of coronary heart disease (CHD) is still lacking. Methods We screened differentially expressed circRNAs (DEcircRNAs) in peripheral blood monocytes (PBMCs) patients CHD, using microarray technology comparing transcriptome. identified upregulated and downregulated circRNAs. same time, we collected patient clinical medical records PBMCs,...
Data on the involvement of proprotein convertase subtilisin/kexin type 9 (PCSK9) and adiponectin in prediabetes progression to 2 diabetes mellitus (T2DM) remains inconclusive. Therefore, this study investigated roles PCSK9 process. This included 1,528 participants with T2DM conducted correlation analyses investigate relationship between levels, pancreatic beta-cell function, insulin levels. Logistic regression analysis receiver operating characteristic (ROC) curves were used determine...
BACKGROUND Esophageal cancer constitutes one of the most aggressive malignant neoplasms associated with poor clinical outcomes. While surgical resection remains cornerstone curative intervention, optimization perioperative care protocols has emerged as an essential strategy to reduce postoperative complications and potentially improve long-term survival rates in patients undergoing esophagectomy. However, substantial debate persists regarding relative importance various risk factors their...
Introduction: It was to investigate the effect of subcutaneous injection low molecular weight heparin (LMWH) sodium on preventing miscarriage in patients with adverse pregnancy and childbirth Serum estradiol (E2), progesterone (P), human chorionic gonadotropin (HCG), D-dimer (D-D) fibrinogen (FIB) levels. Material Method: Eighty-two admitted Wuhai Maternal Child Health Hospital from April 2024 December were randomly grouped, 41 cases each group. Controlgroup(CG) adopted dydrogesterone,...