A5021338112- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Gut microbiota and health
- Helicobacter pylori-related gastroenterology studies
- IL-33, ST2, and ILC Pathways
- Escherichia coli research studies
- Clostridium difficile and Clostridium perfringens research
- Transgenic Plants and Applications
- Mycobacterium research and diagnosis
- Microscopic Colitis
- Asthma and respiratory diseases
- CAR-T cell therapy research
- Amoebic Infections and Treatments
- Immune Response and Inflammation
- Pharmacological Effects of Natural Compounds
- Antimicrobial Peptides and Activities
- Digestive system and related health
- Parasitic Infections and Diagnostics
- Probiotics and Fermented Foods
- Cancer Immunotherapy and Biomarkers
Ajou University
2024-2025
Washington University in St. Louis
2019-2024
Pohang University of Science and Technology
2015-2020
Institute for Basic Science
2015-2020
Dietary antigens are normally rendered nonimmunogenic through a poorly understood "oral tolerance" mechanism that involves immunosuppressive regulatory T (Treg) cells, especially Treg cells induced from conventional in the periphery (pTreg cells). Although orally introducing nominal protein is known to induce such pTreg whether typical diet induces population of under normal conditions thus far has been unknown. By using germ-free mice raised and bred on an elemental devoid dietary antigens,...
Cell wall components of probiotic bacterium Bifidobacterium bifidum drive induction regulatory T cells in the gut.
Both higher- and lower-affinity self-reactive CD4+ T cells are expanded in autoimmunity; however, their individual contribution to disease remains unclear. We addressed this question using peptide-MHCII chimeric antigen receptor (pMHCII-CAR) specifically deplete peptide-reactive mice. Integration of improvements CAR engineering with TCR repertoire analysis was critical for interrogating vivo the role affinity autoimmunity. Our original MOG35-55 pMHCII-CAR, which targeted only higher-affinity...
Generation of tolerogenic peripheral regulatory T (pTreg) cells is commonly thought to involve CD103 + gut dendritic (DCs), yet their role in commensal-reactive pTreg development unclear. Using two Helicobacter - specific cell receptor (TCR) transgenic mouse lines, we found that both and – migratory, but not resident, DCs from the colon-draining mesenteric lymph node presented antigens ex vivo. Loss most migratory vivo using murine genetic models did affect frequency Helicobacter-specific...
T cells proliferate vigorously following acute depletion of CD4+ Foxp3+ regulatory [natural Tregs (nTregs)] and also when naive are transferred to syngeneic, nTreg-deficient Rag1-/- hosts. Here, using mice raised in an antigen-free (AF) environment, we show that proliferation these two situations is directed self ligands rather than food or commensal antigens. In both situations, the absence nTregs elevates B7 expression on host dendritic (DCs) enables a small subset CD4 with high affinity...
Abstract CD4 + T lymphocytes consist of naïve, antigen-specific memory, and memory-phenotype (MP) cell compartments at homeostasis. We recently showed that MP cells exert innate-like effector function during host defense, but whether are functionally heterogeneous and, if so, what signals specify the differentiation subpopulations under homeostatic conditions is still unclear. Here we characterize as consisting T-bet high , low − subsets, with innate, Th1-like activity exclusively associated...
A relatively high affinity/avidity of T cell receptor (TCR) recognition for self-peptide bound to major histocompatibility complex II (self-pMHC) ligands is a distinctive feature CD4 regulatory (Treg) cells, including their development in the thymus and maintenance suppressive functions periphery. Despite such self-reactivity, however, all thymic-derived peripheral Treg populations are neither homogenous phenotype nor uniformly immune-suppressive function under steady state condition. We...
We have previously generated a mouse model of spontaneous Th2-associated disease the small intestine called TRAF6ΔDC, in which dendritic cell (DC)-intrinsic expression signaling mediator TRAF6 is ablated. Interestingly, broad-spectrum antibiotic treatment ameliorates TRAF6ΔDC disease, implying role for commensal microbiota development. However, relationship between drug effects and status remains to be formally demonstrated. To directly assess this relationship, we now bone marrow chimera...
CD4 T cells differentiate into RORγt/IL-17A-expressing in the small intestine following colonization by segmented filamentous bacteria (SFB). However, it remains unclear whether SFB-specific can directly from naïve precursors, and their effector differentiation is solely directed towards Th17 lineage. In this study, we used adoptive cell transfer experiments showed that migrate to intestinal lamina propria (sLP) synthesize IL-17A response SFB colonization. Using single RT-PCR analysis,...
Abstract Immune tolerance in the lung is important for preventing hypersensitivity, such as allergic asthma. Maintenance of established by coordinated activities poorly understood cellular and molecular mechanisms, including participation dendritic cells (DCs). We have previously identified DC expression signaling molecule TRAF6 a non-redundant requirement maintenance immune small intestine mice. Because mucosal tissues share similarities how they interact with exogenous antigens, we...
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Memory-phenotype (MP) CD4 + T lymphocytes develop from naïve cells via self-recognition at homeostasis. While previous studies defined MP as a heterogeneous population that comprises helper 1 (T H 1)/17–like subsets, functional significance of the T-bet − Rorγt subpopulation remains unknown. Here we show whole can differentiate into 1/17/regulatory reg ) to mediate mild and persistent inflammation in lymphopenic environments, whereas exhibit strong, 1-dominated responses. Moreover,...
Abstract CD4+ T lymphocytes consist of naïve, antigen-specific memory, and memory-phenotype (MP) cell compartments in steady state. We recently showed that MP cells exert innate-like effector function host defense. However, it remained to be defined whether population comprises multiple subsets, if so, what factors determine their selective differentiation under homeostatic conditions. Here we characterize as consisting T-bethigh, T-betlow, T-bet− with innate Th1-like activity exclusively...