A5077799127- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Plant Pathogens and Fungal Diseases
- Essential Oils and Antimicrobial Activity
- Pneumocystis jirovecii pneumonia detection and treatment
- Nail Diseases and Treatments
- Bacterial biofilms and quorum sensing
- Enterobacteriaceae and Cronobacter Research
- Urinary Tract Infections Management
- Mycotoxins in Agriculture and Food
- Parasitic Infections and Diagnostics
- Gut microbiota and health
- Pharmaceutical and Antibiotic Environmental Impacts
- Vibrio bacteria research studies
- Reproductive tract infections research
- Nanocomposite Films for Food Packaging
- Diet and metabolism studies
- Herbal Medicine Research Studies
- Antimicrobial agents and applications
- Syphilis Diagnosis and Treatment
- Infections and bacterial resistance
- Dental Anxiety and Anesthesia Techniques
- Occupational health in dentistry
Universidade do Porto
2015-2025
Centre for Health Technology and Services Research
2014-2024
Hospital de São João
2008-2021
Technologies pour la Santé
2021
University of Coimbra
2015
Instituto Piaget
2009
IPO Porto
2001
Lund University
2000
The composition of the essential oil Thymus pulegioides and its antifungal activity on Candida, Aspergillus dermatophyte fungal strains were studied. Essential from aerial parts plant was obtained by hydrodistillation analysed GC GC-MS. showed high contents carvacrol thymol. MIC minimal lethal concentration used to evaluate against Candida (seven clinical isolates four ATCC type strains), [five isolates, two Colección Española de Cultivos Tipo (CECT) strains] five strains. Antifungal...
We have performed the characterization of adhesion profile, biofilm formation, cell surface hydrophobicity (CSH) and antifungal susceptibility 184 Candida clinical isolates obtained from different human reservoirs. Adhesion was quantified using a flow cytometric assay formation evaluated two methodologies: XTT crystal violet assay. CSH with microbial to hydrocarbons test while planktonic assessed accordingly CLSI protocol for yeast M27-A3 S4. Yeast cells non-albicans species exhibit...
ABSTRACT The increasing recognition and importance of fungal infections, the difficulties encountered in their treatment increase resistance to antifungals have stimulated search for therapeutic alternatives. Essential oils been used empirically. essential Thymus ( vulgaris , T. zygis subspecies mastichina ) often folk medicine. aim present study was evaluate objectively antifungal activity according classical bacteriological methodologies − determination minimal inhibitory concentration...
Five new microsatellite loci were described and characterized for use as molecular markers the identification genetic differentiation of Candida albicans strains. Following typing 72 unrelated clinical isolates, analysis revealed that they all polymorphic, presenting from 5 to 30 alleles 8 46 different genotypes. The discriminatory power obtained by combining information generated three microsatellites used in a multiplex PCR amplification strategy was 0.99, highest ever reported. later test...
Candida parapsilosis, an emergent agent of nosocomial infections, was previously made up a complex three genetically distinct groups (groups I, II, and III). Recently, the C. parapsilosis have been renamed as species: sensu stricto, orthopsilosis, metapsilosis. In Portugal, no data pertaining to distribution antifungal susceptibility these species are yet available. present report, we describe incidence metapsilosis among 175 clinical environmental isolates identified by conventional methods...
ABSTRACT In the human fungal pathogen Candida albicans , CUG codon is translated 97% of time as serine and 3% leucine, which potentially originates an array proteins resulting from translation a single gene. Genes encoding cell surface are enriched in codons; thus, mistranslation may influence interactions organism with host. To investigate this, we compared C. strain that misincorporates 28% leucine at CUGs wild-type parental strain. The first displayed increased adherence to inert host...
Biofilms are commonly involved in medical device-related infections. The purpose of this study was to determine the antimicrobial and anti-biofilm activity polyethyleneimine (PEI) PEI-based nanoparticles (nanoPEI) against Staphylococcus aureus, epidermidis, Acinetobacter baumannii Candida albicans (clinical ATCC strains), evaluate their effect upon biofilm formation on polyurethane (PUR)-like catheters. MICs minimal lethal concentrations PEI nanoPEI were determined according CLSI...
ABSTRACT Herein we describe the changes in gene expression profile of Candida parapsilosis associated with acquisition experimentally induced resistance to azole antifungal drugs. Three resistant strains C. were obtained following prolonged vitro exposure a susceptible clinical isolate constant concentrations fluconazole, voriconazole, or posaconazole. We found that after incubation fluconazole became both azoles but not posaconazole, although susceptibility this decreased, whereas strain...
Objectives . The aim of this study was to determine whether irrigation with sodium hypochlorite, chlorhexidine, and ozone gas, alone or in combination, were effective against Enterococcus faecalis Candida albicans ; these are microorganisms frequently isolated from teeth periapical lesions resistant endodontic treatment. Material Methods 220 single root teeth, recently extracted, inoculated formulations tested hypochlorite at 1, 3, 5% chlorhexidine 0.2% 2% gas applied for different periods...
Ibuprofen, a non-steroidal anti-inflammatory drug, exhibited antimicrobial activity against Candida albicans and non-albicans strains. At 10 mg/ml, ibuprofen showed rapid cidal exponential growth phase C. albicans, accompanied by extensive leakage of intracellular K+, permeation to propidium iodide, lysis spheroplasts severe membrane ultrastructural alterations. These results indicate that the killing cells is due direct damage cytoplasmic membrane. 5 inhibited growth; however, it did not...
The polymorphism of a new microsatellite locus (CAI) was investigated in total 114 Candida albicans strains, including 73 independent clinical isolates, multiple isolates from the same patient, several episodes recurrent vulvovaginal infections, and two reference strains. PCR genotyping performed automatically, using fluorescence-labeled primer, 26 alleles 44 different genotypes were identified, resulting discriminatory power 0.97. CAI revealed to be species specific showed low mutation...
ObjectivesThe colonization of indwelling medical devices and subsequent biofilm formation represents a global challenge since it promotes the persistence infection contributes to antimicrobial resistance. The aim this study was determine activity cerium, chitosan hamamelitannin against usual microbial colonizers assess their efficacy regarding on polyurethane (PUR)-like catheters.
Catheter-related bloodstream infections (CRBSIs) are common healthcare-associated associated with increased morbidity and medical costs. Antiseptic- antibiotic-coated central venous catheters (CVCs) have been proposed to reduce the incidence of CRBSIs, variable success. The aim this study was determine in vivo antibiofilm activity biocompatible inexpensive compounds, such as cerium nitrate, chitosan hamamelitannin, against usual agents CRBSIs.The effect hamamelitannin tested Staphylococcus...
The purpose of this study was to unveil whether azole antifungals used in agriculture, similar the clinical azoles humans, can evoke resistance among relevant human pathogens like Aspergillus fumigatus, an ubiquitous agent nature. Additionally, cross-resistance with investigated. Antifungal susceptibility testing environmental and isolates A. fumigatus performed according CLSI M38-A2 protocol. In vitro induction assays were conducted involving daily incubation susceptible isolates, at 35°C...
Abstract The conventional methods used to evaluate chitin content in fungi, such as biochemical assessment of glucosamine release after acid hydrolysis or epifluorescence microscopy, are low throughput, laborious, time‐consuming, and cannot a large number cells. We developed flow cytometric assay, efficient, fast, based on Calcofluor White staining measure yeast A index was defined, its value directly related amount taking into consideration the different levels autofluorecence. Twenty‐two...