A5064064793- Genetic Syndromes and Imprinting
- Medical Imaging and Pathology Studies
- Advanced Electron Microscopy Techniques and Applications
- Parathyroid Disorders and Treatments
- Ion channel regulation and function
- Receptor Mechanisms and Signaling
- Graphene research and applications
- Force Microscopy Techniques and Applications
- Neurotransmitter Receptor Influence on Behavior
- Monoclonal and Polyclonal Antibodies Research
- Genetic and Kidney Cyst Diseases
- Neuroendocrine regulation and behavior
- vaccines and immunoinformatics approaches
- Bacteriophages and microbial interactions
- Cellular Mechanics and Interactions
- Advanced Fluorescence Microscopy Techniques
- Nanopore and Nanochannel Transport Studies
- Toxin Mechanisms and Immunotoxins
- Thyroid and Parathyroid Surgery
- Nanowire Synthesis and Applications
- Dermatological and Skeletal Disorders
- Cystic Fibrosis Research Advances
- Tryptophan and brain disorders
- Metabolism, Diabetes, and Cancer
- Neuroscience and Neuropharmacology Research
Institut de Biologie Structurale
2021-2024
Centre National de la Recherche Scientifique
2015-2024
CEA Grenoble
2021-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2021-2024
Université Grenoble Alpes
2021-2024
Boston Children's Hospital
2024
Harvard University
2024
Université de Montpellier
2019-2020
Institut de Génétique Moléculaire de Montpellier
2015-2020
Institut de Recherche en Infectiologie de Montpellier
2020
Integrins link the extracellular environment to actin cytoskeleton in cell migration and adhesiveness. Rapid coordination between events outside inside is essential. Single-molecule fluorescence dynamics show that ligand binding bent-closed integrin conformation, which predominates on surfaces, followed within milliseconds by two concerted changes, leg extension headpiece opening, give high-affinity conformation. The extended-closed conformation not an intermediate but can be directly...
Solute carrier family 20 member 2 (SLC20A2) and xenotropic polytropic retrovirus receptor 1 (XPR1) are transporters with phosphate uptake efflux functions, respectively. Both associated primary familial brain calcification (PFBC), a genetic disease characterized by cerebral calcium-phosphate deposition neuropsychiatric symptoms. The association of the two same suggests that they jointly regulate fluxes cellular homeostasis, but direct evidence is missing. Here, we found cross-talk between...
The multifactorial origin and neurochemistry of Alzheimer's disease (AD) call for the development multitarget treatment strategies. We report a first-in-class triple acting compound that targets serotonin type 6 3 receptors (5-HT-Rs) monoamine oxidase B (MAO-B) as an approach treating AD. key structural features required MAO-B inhibition 5-HT6R antagonism interaction with 5-HT3R were determined using molecular dynamic simulations cryo-electron microscopy, respectively. Bioavailable PZ-1922...
Abstract Primary familial brain calcification (PFBC) is a rare neurological disease characterized by deposits of calcium phosphate in the basal ganglia and other regions brain. Pathogenic variants XPR1/SLC53A1 gene, which encodes only known inorganic exporter, cause an autosomal dominant form PFBC. These are typically located SPX N-terminal domain protein. Here, we characterize three XPR1 outside PFBC patients with apparently sporadic presentation: c.1375C > T p.(R459C), c.1855A G...
There are very few antiviral drugs available to fight viral infections and the appearance of strains resistant these antivirals is not a rare event.Hence, design new important.We describe prediction peptides with activity (AVP) derived from glycoproteins involved in entrance herpes simplex (HSV) influenza A viruses into their host cells.It known, that during this event suffer several conformational changes due protein-protein interactions, which lead membrane fusion between envelope cellular...
The aim of this study was to design peptides derived from glycoproteins H (gH) and B (gB) herpes simplex viruses type 1 (HSV-1) 2 (HSV-2) with the potential block herpetic infection evaluate their ability inhibit HSV-1 HSV-2 in vitro.A library continuous 15-25 residue stretches (CRSs) located at surface gH gB created. These CRSs were analyzed, only those that highly flexible rich charged residues selected for antiviral (AVPs). toxicity AVPs evaluated by MTT reduction assays. Virucidal...
Thymocyte differentiation is dependent on the availability and transport of metabolites in thymus niche. As expression metabolite transporters a rate-limiting step nutrient utilization, cell surface transporter levels generally reflect cell's metabolic state. The GLUT1 glucose upregulated actively dividing thymocytes, identifying thymocytes with an increased metabolism. However, it not clear whether essential elements such as phosphate are modulated during thymocyte differentiation. While...
Summary Vortioxetine (VTX) is a recent antidepressant that targets variety of serotonin receptors. We investigate the drug’s molecular mechanism operation at 5-HT 3 receptors (5-HT R), which features two mysterious properties: VTX acts differently on rodent and human R; appears to suppress any subsequent response agonists. Using combination cryo-EM, electrophysiology, dynamics, we show stabilizes resting inhibited state mouse R an agonist bound-like R, in line with functional profile drug....
Graphene is an excellent support film for high-resolution transmission electron microscopy (TEM) but its use with biological samples, notably in cryo-TEM, hindered by inherent hydrophobicity. Whereas surface treatments have been proposed to render graphene hydrophilic, they are often difficult reproduce due a lack of information on the structural changes that modify wetting properties graphene. This study aims correlate atomic structure allow reproducible protocol advance application...