A5040099521- Seaweed-derived Bioactive Compounds
- Marine and coastal plant biology
- Algal biology and biofuel production
- Microbial Natural Products and Biosynthesis
- Echinoderm biology and ecology
- Enzyme Production and Characterization
- Invertebrate Immune Response Mechanisms
- Glycosylation and Glycoproteins Research
- Aquaculture disease management and microbiota
- Carbohydrate Chemistry and Synthesis
- Marine Sponges and Natural Products
- Genomics and Phylogenetic Studies
- Vibrio bacteria research studies
- Probiotics and Fermented Foods
- Fungal Biology and Applications
- Traditional and Medicinal Uses of Annonaceae
- Food Industry and Aquatic Biology
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Microbial Metabolism and Applications
- Moringa oleifera research and applications
- Microbial Community Ecology and Physiology
- Microbial Metabolites in Food Biotechnology
Vietnam Academy of Science and Technology
2016-2024
Nha Trang University
2022-2024
Tanta University
2023
Technical University of Denmark
2020-2022
Fucoidans from brown macroalgae (brown seaweeds) have different structures and many interesting bioactivities. are classically extracted seaweeds by hot acidic extraction. Here, we report a new targeted enzyme-assisted methodology for fucoidan extraction seaweeds. This protocol involves one-step combined use of commercial cellulase preparation (Cellic®CTec2) an alginate lyase Sphingomonas sp. (SALy), reaction at pH 6.0, 40 °C, removal non-fucoidan polysaccharides Ca2+ precipitation,...
Fucoidanases are endo-fucoidanases (also known as endo-fucanases) that catalyze hydrolysis of α-glycosidic linkages in fucoidans, a family sulfated fucose-rich polysaccharides primarily found the cell walls brown seaweeds. promising tools for producing bioactive fucoidan oligosaccharides range biomedical applications. High sulfation degree has been linked to high bioactivity fucoidans. In this study, novel fucoidanase, Fhf2, was identified genome aerobic, Gram-negative marine bacterium...
Fucoidans from brown macroalgae are sulfated fucose-rich polysaccharides, that have several beneficial biological activities, including anti-inflammatory and anti-tumor effects. Controlled enzymatic depolymerization of the fucoidan backbone can help produce homogeneous, defined products for structure-function research pharmaceutical uses. However, only a few endo-fucoidanases been described. This article reports genome-based discovery, recombinant expression in Escherichia coli,...
Seven new deoxyisoaustamide derivatives (1–7) together with known compounds (8–10) were isolated from the coral-derived fungus Penicillium dimorphosporum KMM 4689. Their structures established using spectroscopic methods, X-ray diffraction analysis and by comparison related compounds. The absolute configurations of some alkaloids determined based on CD NOESY data as well biogenetic considerations. cytotoxic neuroprotective activities examined structure-activity relationships pointed out. New...
Endo-fucoidanases, including EC 3.2.1.211 endo-α-1,3-L-fucanase and 3.2.1.212 endo-α-1,4-L-fucanase activities, catalyze depolymerization of fucoidans - a group bioactive, sulfated fucosyl-polysaccharides found primarily in brown macroalgae (brown seaweeds). Quantitative assessment endo-fucoidanase activity is critical for characterizing kinetics comparing the action different endo-fucoidanases on types fucoidans. However, current state-of-the-art assay consists qualitative based...
Vibriosis in farmed animals is a serious threat to aquaculture worldwide. Using probiotics and anti-Vibrio antimicrobial substances systems can be means of preventing Vibrio infections. Therefore, we aimed characterize compare 16 potential (Vi+) isolated from marine sponges fish intestines collected the Vietnam Sea, as well an bacteriocin fully explore their application potentials. 16S rRNA sequencing confirmed all Vi+ Bacillus species with different strain variants across two sample types....
Marine fungi have become an important source of bioactive natural products. The present study was concerned with the screening antimicrobial activity from 73 fungal strains isolated various marine habitats collected five different localities at Son Tra Peninsula, Da Nang, Vietnam. For first step screening, ethyl acetate extract each isolate prepared and their against human microbial pathogens investigated using disc diffusion method. panel used were Bacillus cereus ATCC 11778, Escherichia...
Abstract Carboxymethyl kappa carrageenans (CMKCs) have been synthesized from carrageenan extracted the red alga Kappaphycus striatus with different molar ratios of monochloroacetic acid (MCA) in presence sodium hydroxide. The structural and physicochemical characteristic products wase evaluated by FT-IR, 13 C 1 H NMR spectra, degree substitution, molecular weight, zeta potential biological activity. Degree substitution carboxymethyl group ranged 0.24 to 1.12. FT-IR spectra proved...
<title>Abstract</title> The red alga Betaphycus gelatinus showed a monthly little variation in carrageenan yields, 3,6 anhydro galactose and sulfate contents for the native carrageenans (68.2 – 73.1%, 23.1-24.8% 19.5 20.3%, respectively) alkali modified (23.7 31.5%, 41.6 43.9% 8.6 8.7%, respectively). gelling, melting temperatures gel strength of from April to June no change, with recorded values 40.8 o C, 74.5 C 310 g cm-2 , respectively. molecular weight was primarily distributed at 876...
Aqueous extracts from 21 species of Vietnam marine invertebrates, including 11 bivalve and 10 gastropod species, were examined for haemagglutination activity using native enzyme-treated different animal human erythrocytes. The 8 found to have haemagglutinination activities toward at least one type erythrocyte tested. A total 86% invertebrate surveyed active. Strong was detected in two (Tridacna squamosa Geloina coaxans) three (Tutufa rubeta, Pleuroploca trapezium Tectus conus) with rabbit,...
The fucoidanase from the digestive glands of marine shell Vasticardium flavum was studied. catalysed hydrolysis 1→3-L-fucan sea cucumbers Stichopus variegatus, Holothuria spinifera, did not catalyze fucoidan F. evanescens and vesiculosus containing alternating α-1→4 α-1→3 glycoside bonds. This enzyme also U. pinnatifida, S. mcclurei, which belong to galactofucan group. Optima pH time incubation were at 3-4 24 hours, respectively. activity significantly increased in presence Ca2+, Ba2+, Co2+...
Sponge associated marine microbes recognised as potential candidate for screening and isolation of bioactive compounds. In this work, thirty-one bacterial strains with seven species sponges collected from Phu Quoc Island, Vietnam were isolated screened antimicrobial activity against selected human animal pathogens including Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Vibrio parahaemolyticus, harveyi, Bacillus cereus, Streptococcus faecalis, Listeria monocytogenes,...
The biosynthesis of compounds with antibiotic activity produced by marine fungi, strongly depends on their growth conditions. A good understanding the role culture conditions in metabolites may lead to better exploitation microbial metabolites. In this study, influence including incubation period, initial pH and salinity antimicrobial secondary production fungus 01NT.1.1.5 was investigated. This isolate, obtained from sponge Stylissa sp. Nha Trang Bay, exhibited a broad spectrum vitro...
Mangrove-derived fungi are the potential source of novel metabolites, comprising unique molecular structures with diverse bioactivities. In this study, 32 fungal strains isolated from Tan Dao mangrove forest in Khanh Hoa province were investigated for their ability to produce antimicrobial agents using disc diffusion method. Seventy-eight percent obtained (25 out isolates) exhibited activity against at least two test pathogens. Of them, bacteria Bacillus cereus and Streptococcus faecalis...
A new lectin from the marine red alga Gracilaria canaliculata (GCL) was isolated by a combination of aqueous ethanol extraction, precipitation, ion exchange and filtration chromatography. Lectin gave single band with molecular mass 22,000 Da in both non-reducing reducing SDS-PAGE conditions, indicating that GCL is monomeric protein. The hemagglutination activities were stable over wide range pH 3 to 10, temperature rose 60 °C it not affected either presence EDTA or addition divalent cations....
Screening antibacterial activity of fifty marine bacterial strains isolated from twenty-three species sponges Phu Quoc island. Among them, twenty-one (42%) have the ability to inhibit effectively at least two ten tested bacteria. Especially, strain 045-203-4 showed strong against six pathogenic bacteria for humans and organisms, viz. Pseudomonas aeruginosa, Escherichia coli, Vibrio harveyi, Klebsiella pneumoniae, Bacillus cereus Streptococcus faecalis. The produced with high in medium...
A new lectin from the marine red alga Gracilaria canaliculata (GCL) was isolated by a combination of aqueous ethanol extraction, precipitation, ion exchange and filtration chromatography. Lectin gave single band with molecular mass 22,000 Da in both non-reducing reducing SDS-PAGE conditions, indicating that GCL is monomeric protein. The hemagglutination activities were stable over wide range pH 3 to 10, temperature up 60 oC not affected either presence EDTA or addition divalent cations. had...