A5102016068- Microscopic Colitis
- Inflammatory Bowel Disease
- Immunotherapy and Immune Responses
- Liver Disease Diagnosis and Treatment
- Hepatitis B Virus Studies
- Molecular Biology Techniques and Applications
- Bacteriophages and microbial interactions
- Monoclonal and Polyclonal Antibodies Research
- Antimicrobial Peptides and Activities
- SARS-CoV-2 detection and testing
- Helicobacter pylori-related gastroenterology studies
- Viral gastroenteritis research and epidemiology
- Protein purification and stability
- IL-33, ST2, and ILC Pathways
- Selenium in Biological Systems
- RNA Interference and Gene Delivery
- Respiratory viral infections research
- Influenza Virus Research Studies
- Immune Cell Function and Interaction
- Hepatitis C virus research
China Medical University
2009-2017
It is well accepted that vaccination by oral administration has many advantages over injected parenteral immunization. The present study focuses on whether with a DNA vaccine could induce protective immunity against respiratory challenge infection. M1 gene of influenza A virus was used to construct using pcDNA 3.1(+) plasmid, eukaryotic expression vector. cationic liposomes were deliver the constructed vaccine. In vitro and in vivo observed cell line intestine orally vaccinated C57BL/6 mice,...
Background. The role of the Th17/Treg balance in development experimental colitis remains poorly understood. Methods. We exploited differential response BALB/c mice and C57BL/6 towards drinking water mediated by dextran sulfate sodium (DSS) challenge. Results. DSS-resistant were characterized low levels IFN- γ TNF- α but high IL-4, IL-6, IL-10, IL-17A, IL-17F, colon lamina propria mesenteric lymph node (MLN) CD4 + CD25 FoxP3 T cells when compared to mice. Collectively, these data indicate...
Abstract Background Liver fibrosis progression is commonly found in patients with CHB. biopsy a gold standard for identifying the extent of liver fibrosis, but has many draw-backs. It essential to construct noninvasive model predict levels risk fibrosis. would provide very useful information help reduce number biopsies CHB patients. Methods 339 chronic hepatitis B HBsAg-positive were investigated retrospectively, and divided at random into 2 subsets twice as training set validation set; 116...
To assess the effect of sodium selenite on severity dextran sulfate (DSS)-induced colitis in C57BL/6 mice.Mice were randomly divided into four groups (n = 10/group): normal group, selenium (Se) chronic and Se + group. The mice sacrificed day 26. Survival rates, clinical symptoms, colon length, histological changes determined. percentages absolute numbers immune system cells lamina propria lymphocytes (LPL) colon, expression mRNA tissue, concentrations Th1, Th17, Treg cytokines LPL from large...
Objective To detect the distribution of expression products Ag85A DNA vaccine in spleen. Methods E. coil DH5α was transformed by pCDNA3. 1 +-Ag85A. After extraction and purification re-comhinant p]asmid with cndotoxin free kit, liposome encapsulated recombinant pre-pared. C57BL/6 mice were randomly divided into two groups, test group control group, respectively. The immunized through intragastric administration once weeks for three times. sacri-riced after final vaccine. spleen detected...