A5109781832- Blood groups and transfusion
- Erythrocyte Function and Pathophysiology
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Platelet Disorders and Treatments
- Hemoglobinopathies and Related Disorders
- Immunodeficiency and Autoimmune Disorders
- Blood disorders and treatments
- Cancer Research and Treatments
- 3D Printing in Biomedical Research
- Heparin-Induced Thrombocytopenia and Thrombosis
- Cancer Cells and Metastasis
- Autophagy in Disease and Therapy
Guangzhou University of Chinese Medicine
2024
Shanghai Jiao Tong University
2024
Shanghai Blood Center
2006-2019
Lanzhou Army General Hospital
2017
East China Normal University
2008
National Institutes of Health
2006
The purpose of the work was to analyse genotype D-elute (DEL) samples and elucidate whether there were novel DEL alleles in Chinese population.D-negative identified by an indirect antiglobulin test (IAT), absorption\elution tests screen weak D, partial D phenotypes. DELs further analysed multiplex PCR, PCR-sequence-specific primers (PCR-SSP) sequencing. Some determined show RHD zygosity PCR-restriction fragment length polymorphism or real-time quantitative PCR.Of 400 253 from individual...
Impaired tumor cell antigen presentation contributes significantly to immune evasion. This study identifies Berbamine hydrochloride (Ber), a compound derived from traditional Chinese medicine, as an effective inhibitor of autophagy that enhances in cells. Ber increases MHC-I-mediated melanoma cells, improving recognition and elimination by CD8+ T Mutation Atg4b, which blocks autophagy, also raises MHC-I levels on the surface, further treatment with under these conditions does not increase...
summary In order to determine gene frequencies of human platelet antigen (HPA) and establish a panel accredited HPA‐1a, ‐2a, ‐4a, ‐5a ‐6a‐negative donors as well an HPA‐typed donor registry, total 1000 Chinese Han nationality (500 from north China 500 south China) were typed for HPA‐1 through ‐16 using DNA‐based polymerase chain reaction with sequence‐specific primers genotyping method. The HPA‐1b , ‐2b ‐3b ‐4b ‐5b ‐6bw ‐10bw ‐15b 0·0060, 0·0485, 0·4055, 0·0045, 0·0140, 0·0135, 0·0005...
Background and Objectives RHD alleles are considered variable in the Chinese RhD‐negative persons. The purpose of present work was to elucidate molecular bases two novel identified a survey population. Materials Methods A total 163 blood samples were investigated. sequences exons examined by exon specific multiplex polymerase chain reaction (PCR) PCR with sequence‐specific primers (PCR‐SSP). Characterizations intron 2 Rhesus box performed PCR‐PstI digestion. DNA cDNA determined reverse...
The D antigen is the most antigenic in Rh blood group system. Variation may have potential to cause alloimmunization. variant different molecular backgrounds people of ethnic groups. aim this study was investigate serological and differences related among Chinese Blood samples six races Xinjiang were screened for variants using test. suspected further analyzed by polymerase chain reaction sequencing determine RHD genotype. Fourteen elute phenotypes (DELs), included 11 Han, 2 Uigur 1 Hui,...
This work aims to develop a multiplex polymerase chain reaction combined with DNA pooling for mass screening rare blood types.The differences in most group antigens are associated single-nucleotide polymorphisms (SNPs), which used detecting antigen expression at the molecular level. However, all existing sequence-specific primers (PCR-SSP) assays typing genotype one or several SNPs individually. is way that reduces amount of genotyping required.A sensitive PCR-SSP assay testing pooled was...
The novel HLA ‐A*02:06:13 allele shows one nucleotide difference from A *02:06:01 in exon 4 at position 657 ( T → C ).
The newly detected HLA ‐B*40:210 allele has two nucleotide changes in exon 2 compared to B*40:49 allele.
SUMMARY Objectives We aimed to analyse the molecular backgrounds of family in which an eight‐day‐old baby was confirmed have hemolytic disease newborn (HDN) and phenotype observed for did not conform expected phenotype. Background The silent RHCE allele is rare Rh system. Methods To determine antibody specificity, her members' blood samples were collected tested using routine serological methods. C + c‐e E ‐ based on maternal RhC‐c e‐ RH genes members further analysed by sequencing. Results...
HLA ‐A*11:125 allele differs from A*11:01:01 by one nucleotide substitution at nt 140 T to C.
HLA-A*11:141 has 1 nucleotide change from HLA-A*11:01:01 at position 470G>C.
Abstract Circulating tumor cells (CTCs) are cancer that shed from the primary and enter into body fluids of patient, where they travel to distant sites ultimately form metastasis. Understanding biology CTCs, in particular at critical stages their itinerary, holds promises for better cure. Since beginning this century, liquid biopsy has steadily grown be a keen area research due its non-invasive features. As one most promising biomarkers, CTCs have shown great potential diagnosis, prognosis,...