A5044765206- Cancer Immunotherapy and Biomarkers
- Melanoma and MAPK Pathways
- Immunotherapy and Immune Responses
- Cancer-related Molecular Pathways
- Cancer Cells and Metastasis
- Cutaneous Melanoma Detection and Management
- RNA Research and Splicing
- Cytokine Signaling Pathways and Interactions
- Liver Disease Diagnosis and Treatment
- Diet and metabolism studies
- Immune Cell Function and Interaction
- Cancer Mechanisms and Therapy
- Microtubule and mitosis dynamics
- Hematopoietic Stem Cell Transplantation
- Amino Acid Enzymes and Metabolism
- Ubiquitin and proteasome pathways
- Hemoglobinopathies and Related Disorders
- Blood groups and transfusion
- Cancer Research and Treatments
- Alcohol Consumption and Health Effects
- Nuclear Structure and Function
- Ocular Oncology and Treatments
- Protein Degradation and Inhibitors
Rush University Medical Center
2021-2024
Purdue University West Lafayette
2021
Medical University of Vienna
2014-2017
Abstract In melanoma, therapies with inhibitors to oncogenic BRAF V600E are highly effective but responses often short-lived due the emergence of drug-resistant tumor subpopulations. We describe here a mechanism acquired drug resistance through microenvironment, which is mediated by human tumor-associated B cells. Human melanoma cells constitutively produce growth factor FGF-2, activates tumor-infiltrating IGF-1. B-cell-derived IGF-1 critical for melanomas and MEK heterogeneous...
Objectives: Only 20- 30% of individuals with Alcohol Use Disorder (AUD) develop Alcoholic Liver Disease (ALD). While the development gut-derived endotoxemia is understood to be a required co-factor, increased intestinal permeability in ALD not completely understood. Methods: We recruited 178 subjects – 58 healthy controls (HC), 32 ALD, 53 AUD but no liver disease (ALC), and 35 nonalcoholic fatty (NAFLD). Intestinal was assessed by sugar cocktail as percentage oral dose. The test repeated...
Abstract MITF (microphthalmia-associated transcription factor) is a frequently amplified lineage-specific oncogene in human melanoma, whose role intrinsic drug resistance has not been systematically investigated. Utilizing chemical inhibitors for major signaling pathways/cellular processes, we witness as an elicitor of resistance. To search kinase(s) targets able to bypass MITF-conferred resistance, employed multi-kinase inhibitor-directed proteomics-based differential affinity screen...
Activity of both c-Jun and cyclin D1 is deemed critical for melanoma cell proliferation. This functionality corroborated by frequently elevated expression activity these proteins in human melanomas. Correspondingly, alleviating function vital to the success antimelanoma therapeutics.To understand role N-terminal kinase (JNK) signalling pathway proliferation survival.The effect JNK inhibitors SP600125 JNK-IN-8 on survival genetically highly representative lines was studied assays apoptosis....
Melanoma, the deadliest form of skin cancer, is highly immunogenic and frequently infiltrated with immune cells including B cells. The role tumor-infiltrating (TIBCs) in melanoma as yet unresolved, possibly due to technical challenges obtaining TIBCs sufficient quantity for extensive studies limited life span vitro. A comprehensive workflow has thus been developed successful isolation proteomic analysis a low number from fresh, human tissue. In addition, we generated vitro-proliferating TIBC...
Abstract MITF is a frequently amplified lineage-specific oncogene in human melanoma. Current drug discovery effort has, however, paradoxically ignored its criticality melanoma cell biology and has failed to address MITF-associated intrinsic acquired resistance. Here, search the kinase targets able circumvent MITF-conferred resistance, we used multi-kinase inhibitor-directed chemical proteomics-based differential target affinity screen immortalized melanocytes carrying ectopic overexpression....
Abstract For women, breast cancer is the most diagnosed cancer, with metastasis being primary cause of cancer-related deaths. During metastasis, cells detach from extracellular matrix (ECM) to move a secondary site. Unlike normal cells, metastatic are resistant cell death upon ECM detachment. The amino acid glutamine used as an energy source during progression via replenishing TCA cycle. Breast have decreased viability in absence glutamine, but effect detached conditions not known. We...
Introduction: Passenger Lymphocyte Syndrome (PLS) is a subtype of graft-versus-host disease that can occur days to weeks after stem cell transplants or solid organ transplantation an ABO-mismatched organ. Antibodies produced from the transfer donor B-lymphocytes target recipient red cells and cause hemolysis. The true incidence PLS unknown, as many cases are subclinical go undetected. Classically, develops with minor ABO-incompatible transplantation, when type O donated A blood, ABO...