A5086815060- Statistical Methods and Inference
- Statistical Methods and Bayesian Inference
- Zebrafish Biomedical Research Applications
- Bayesian Methods and Mixture Models
- Advanced Causal Inference Techniques
- Gene expression and cancer classification
- Bioactive Compounds and Antitumor Agents
- Angiogenesis and VEGF in Cancer
- Statistical Distribution Estimation and Applications
- Health Systems, Economic Evaluations, Quality of Life
- Nephrotoxicity and Medicinal Plants
- Systemic Sclerosis and Related Diseases
- Cardiomyopathy and Myosin Studies
- Statistical Methods in Clinical Trials
- Hydrological Forecasting Using AI
- Algorithms and Data Compression
- Neuroinflammation and Neurodegeneration Mechanisms
- Biological and pharmacological studies of plants
- Viral Infectious Diseases and Gene Expression in Insects
- Optimal Experimental Design Methods
- Aquaculture disease management and microbiota
- Genetic Mapping and Diversity in Plants and Animals
- Drug-Induced Hepatotoxicity and Protection
- Protein Tyrosine Phosphatases
- Muscle Physiology and Disorders
Tamkang University
2012-2024
Hungkuang University
2013-2014
National Health Research Institutes
2005-2009
Chung Yuan Christian University
2009
National Cheng Kung University
2003
The nephrotoxicity of aristolochic acid (AA) is well known, but information regarding the attenuation AA-induced toxicity limited. aim present study was to nephroprotective effects resveratrol (Resv) and ursolic (UA) in a zebrafish model. We used two transgenic lines, Tg(wt1b:EGFP) Tg(gata1:DsRed), evaluate Resv UA by recording subtle changes kidney red blood cell circulation. Our results demonstrated that both treatment can attenuate malformations improve Glomerular filtration rate assays...
Caffeine is a widely consumed substance that occurs in numerous dietary sources, but teratogenic effects of caffeine intake during embryonic development are still not clear. In the present study, we used zebrafish as model to assess caffeine-induced toxicity on vascular development. A green fluorescent endothelium transgenic line, Tg(fli1:egfp), was utilized for sensitive detection development, including vasculo- and angiogenesis. Caffeine-treated embryos showed no defects vasculogenesis,...
The aim of this study was to investigate novel chalcones with potent anti-inflammatory activities in vivo. Chalcone and two chalcone analogues (compound 5 9) were evaluated using a caudal fin-wounded transgenic zebrafish line "Tg(mpx:gfp)" visualize the effect neutrophil recruitment dynamically. Results showed that treatment compound 9 not only affected wound-induced recruitment, but also Mpx enzymatic activity. Moreover, protein expression levels pro-inflammatory factors (Mpx, NFκB, TNFα)...
Protein tyrosine phosphatase 1β (PTP-1β) is involved in the regulation of several important physiological pathways. It regulates both insulin and leptin signaling, interacts with epidermal- platelet-derived growth factor receptors. The gene located on human chromosome 20q13, rare single nucleotide polymorphisms (SNPs) have been shown to be associated resistance diabetes different populations. As part our ongoing investigations into genetic basis hypertension, we examined common sequence...
Baculoviruses, a family of large, rod-shaped viruses that mainly infect lepidopteran insects, have been widely used to transduce various cells for exogenous gene expression. Nonetheless, how virus controls its transcription program in is poorly understood. With custom-made baculovirus DNA microarray, we investigated the recombinant Autographa californica multiple nucleopolyhedrosis (AcMNPV) expression Sf21 over time course infection. Our analysis kinetics uncovered sequential viral patterns...
Owing to the fact that general semiparametric inference procedures are still underdeveloped for multivariate interval-censored event time data, we pro- pose maximum likelihood estimation gamma-frailty Cox model under mixed-case interval censoring. We establish consistency of estimator (SPMLE) parame- ters, including regression coefficients and cumulative hazard functions in model, variance gamma frailty. The SPMLEs cumu- lative shown have a n 1=3 -rate convergence, while those frailty 1=2...
We used ultraviolet (UV)-induced fin damage in zebrafish as a system for evaluating the chemopreventive potential of flavonoids. Chemopreventive effects each compound, including flavone, flavanone, and chalcone, on development were evaluated using Kaplan-Meier analysis Cox proportional hazards regression. Results showed that 1) flavone has highest capacity to protect fins from UV-induced damages among other groups; 2) UV+1 ppm group are 1.02~9.60 times more likely return normal than ones...
This study applied broccoli and cauliflower extracts (whole, floret, stem) to zebrafish larvae in parallel receive 100 mJ/cm2 of UVB six times, recorded their fin malformation phenotypes. Chemopreventive effects each group, including UVB, whole-, floret-, stem-extracts on development were evaluated using Kaplan-Meier analysis, log-rank test, Cox proportional hazards regression. Results showed that (1) fins the + whole extract group are 6.20~9.32-times more likely return normal than ones only...
Abstract The objective of this study was to investigate the embryotoxicity diclofenac. Zebrafish ( Danio rerio ) embryos at 12 hpf were treated with different dosages diclofenac (0–2,000 ppm) for time courses (12–72 hr). Results showed no evident differences in survival rates or morphological changes between mock‐treated control (0 zebrafish and those 1‐ppm diclofenac‐exposure (12–24, 12–36 hpf). In contrast, after higher doses (5 10 exposure, displayed some defective phenotypes, including...
Abstract 4‐methylimidazole (4‐MI) is an imidazole‐derived organic chemical compound that can be used as a raw material in the manufacture of diverse chemicals and has been identified ingredient caramel color soybean sauce, beers, other soft drinks. The aim present study was to investigate teratogenic effects 4‐MI during zebrafish embryogenesis. Zebrafish embryos were treated with different dosages (0‐120 mM) for exposure durations (12‐60 hours). percentages malformed phenotypes increased...
(1) Background: Roselle (Hibiscus sabdariffa) is a flowering plant reported to have anti-obesity, antioxidant, antibacterial, and anti-inflammatory effects. This study aims evaluate the UV-absorbing antioxidant activities of roselle aqueous extracts (RAE) test protective effects RAE against UV radiation in zebrafish embryos. (2) Methods: DPPH assay UV-spectrum methods were applied activities, respectively. The evaluated using fin morphology recording, Kaplan–Meier analysis, Cox proportional...
AIM:To investigate the long-term consequences of chemotherapy-related HBV reactivation in patients with lymphoma. METHODS:This study was based on database published prospective evaluating lymphoma during chemotherapy.Deteriorated liver reserve (DLR) defined as development either one following conditions follow-up: (1) newly onset parenchyma disease, splenomegaly or ascites without evidence involvement; (2) decrease ratio (albumin/globulin ratio) to less than 0.8 increase INR prothrombin time...
Abstract. This paper describes our studies on non‐parametric maximum‐likelihood estimators in a semiparametric mixture model for competing‐risks data, which proportional hazards models are specified failure time conditional cause and multinomial is the marginal distribution of covariates. We provide verifiable identifiability condition and, based it, establish an asymptotic profile likelihood theory this model. also efficient algorithms computation estimate its variance. The success method...