A5003558872- Pneumonia and Respiratory Infections
- Microbial infections and disease research
- Respiratory viral infections research
- Antibiotic Resistance in Bacteria
- Immune Response and Inflammation
- Lung Cancer Research Studies
- Antibiotics Pharmacokinetics and Efficacy
- Pneumocystis jirovecii pneumonia detection and treatment
- Mycobacterium research and diagnosis
Beijing Friendship Hospital
2018-2025
Capital Medical University
2019-2025
Beijing Fengtai Hospital
2025
Research Institute for Tropical Medicine
2021
Peking University
2018
Meitan General Hospital
2018
Peking University Third Hospital
2018
Abstract This paper retrospectively analysed the prevalence of macrolide-resistant Mycoplasma pneumoniae (MRMP) in some parts China. Between January 2013 and December 2019, we collected 4,145 respiratory samples, including pharyngeal swabs alveolar lavage fluid. The highest PCR-positive rate M. was 74.5% Beijing, resistance 100% Shanghai, Gansu lowest with 20%. 2013, MRMP 97.4% 2019; for adults Beijing 17.9% 10.48%. Among children diagnosed community-acquired pneumonia (CAP), rates were both...
Introduction Mycoplasma pneumoniae is one of the important pathogens community-acquired pneumonia (CAP), and P1 adhesin serves as a pathogenic protein an immune involved in pathogenesis mycoplasma . The aim this study was to investigate genotype its association with disease severity patients CAP from 2017 2019. Methods M. identified patient samples by real-time quantitative polymerase chain reaction (qPCR). genotypes were determined using culture-independent typing method. Results In total,...
ABSTRACT To analyze the characteristics of Mycoplasma pneumoniae as well macrolide antibiotic resistance through whole-genome sequencing and comparative genomics. Thirteen clinical strains isolated from 2003 to 2019 were selected, 10 which resistant erythromycin (MIC >64 µg/mL), including 8 P1-type I 2 II. Three sensitive (<1 µg/mL) One strain had an A→G point mutation at position 2064 in region V 23S rRNA, others it 2063, while three no here. Genome assembly genome analysis revealed a...
Mycoplasma pneumoniae (MP) is an important human pathogen that mainly affects children causing general and severe pneumonia (G/SMPP). In the present study, a comprehensive immune response data (33 cytokines) was obtained in school-age (3–9 years old) during MPP, aiming to analyze patterns MPP. At acute phase, changes of cytokines were both detected GMPP (24/33) SMPP (23/33) groups compared healthy group (p &lt; 0.05), with 20 identical cytokines. Between MPP groups, levels 13 (IL-2,...
Background: Mycoplasma pneumoniae is an important infectious pathogen of lower respiratory tract infection in children and adolescents. Macrolide resistant M. (MRMP) has become increasingly prevalent, identifying resistance genes crucial for treatment. Case Presentation: We report a patient with severe refractory pneumonia (MPP). The failure initial clinical treatment prompted the re-analysis metagenomic next-generation sequencing (mNGS) data macrolide-resistant gene. Macrolide-resistance...
Mycoplasma pneumoniae is a leading cause of community-acquired respiratory infections. Infantile Feire Kechuan Oral Solution (IFKOS) effective for treatment M. infection. The aim this study was to explore the potential mechanism IFKOS against infection in basal epithelial human lung adenocarcinoma A549 cells.The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay used determine effects on viability cells infected with pneumoniae. Optical microscopy observe cell morphology and...
The aim of this study was to evaluate the inhibitory effect antibiotics and Xiao'er Feire Kechuan Oral Solution on Mycoplasma pneumoniae (MP) clinical isolates.Twenty isolates containing A-to-G transition at position 2063 10 without mutations in 23S rRNA V regions were randomly selected. international standard strain FH chosen as control strain. minimum concentration (MIC) macrolide, quinolones, tetracycline, MP performed using broth microdilution method.In vitro antibiotic susceptibility...
Mycoplasma pneumoniae (MP) is an important respiratory pathogen, the prevalence of macrolide-resistant MP (mainly containing A2063G mutation in 23S rRNA) increased recent years. Epidemiological studies suggest a higher type I resistant (IR) strains than corresponding sensitive (IS/IIS) strains, but not II (IIR) strains. Here, we aimed to analyze factors underlying altered IR First, proteomic analyses exhibit protein compositions were specific, while more differential proteins detected...
Objective To developed A laboratory diagnosis of Moraxella catarrhalis by an laboratories diagnostic method real-time fluorescence quantitative PCR assay. Methods The specific primers and probes were designed based on the sequence outer membrane protein CopB(copB)gene in catarrhalis, Taqman probe RT-PCR was to detect catarrhalis.The standard plasmids extracted from strains used constitute samples, compared with these sensitivity assay tested established curves.The specificity DNA...
Older children especially from seven to thirteen years old are more prone develop Mycoplasma pneumoniae (MP) infection; in winter susceptible infect with MP. In Beijing, China 2016 the rates of macrolide resistance MP were 69.48% (in total children), 61.59% outpatients) and 79.28% hospitalized patients), respectively. All resistant isolates harbored A2063G or A2064G mutation 23S rRNA gene. Seven showed a mixed infection. Susceptibility results that 73 demonstrated different levels...
Objective To investigate the antibacterial effect of Fusidic acid on Mycoplasma pneumoniae and antibiotic resistant in vitro. Methods Twenty- eight clinical strains isolated from patients with respiratory tract infection at Beijing Friendship Hospital Affiliated to Capital University Medical Sciences January December 2016 2 reference were enrolled.The minimum inhibitory concentration (MIC) Azithromycin determined by using micro-dilution ration method.The chessboard method was used...
Abstract This study aims to evaluate laboratory methods for the diagnosis of Mycoplasma pneumoniae . Ninety-three children infected with were tested, and large sample size makes results representative. The pharyngeal swabs serum samples mostly collected from first week disease, or second third disease in recovery children. healthy cultured two them positive bacterium. accuracy diagnostic evaluated against test 4-fold increase decrease titers antibody samples. specificity culture method was...
Abstract Background: To investigate the drug resistance and clinical characteristics of hospitalized children with drug-resistant Mycoplasma pneumoniae pneumonia (MRMP). Methods: Sixty patients MPP admitted to Second Pediatric Respiratory Ward Shengjing Hospital, Affiliated China Medical University from November 2016 February 2017 were enrolled in study. Results: Of these 53/60 (88.3%) had nucleic acid identified by throat swab. 23S rRNA V region gene sequencing was performed, 47/49 (95.9%)...