A5082575471- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- Genomics and Chromatin Dynamics
- Lipid Membrane Structure and Behavior
- Nuclear Structure and Function
- Connexins and lens biology
- Enzyme Structure and Function
Texas A&M University
2023-2024
The University of Texas Health Science Center at San Antonio
2024
Indian Institute of Technology Gandhinagar
2023
The FET protein family, comprising FUS, EWS, and TAF15, plays crucial roles in mRNA maturation, transcriptional regulation, DNA damage response. Clinically, they are linked to Ewing family tumors neurodegenerative diseases such as amyotrophic lateral sclerosis. fusion EWS::FLI1, the causative mutation of sarcoma, arises from a genomic translocation that fuses portion low-complexity domain (LCD) EWS (EWSLCD) with binding ETS transcription factor FLI1. This modifies programs disrupts native...
Abstract Cells harbor numerous mesoscale membraneless compartments that house specific biochemical processes and perform distinct cellular functions. These protein RNA-rich bodies are thought to form through multivalent interactions among proteins nucleic acids resulting in demixing via liquid-liquid phase separation (LLPS). Proteins harboring intrinsically disordered regions (IDRs) predominate organelles. However, it is not known whether IDR sequence alone can dictate the formation of...
Ewing sarcoma, the second most common pediatric bone and soft tissue cancer, is caused by aberrant fusion of EWS low-complexity domain (EWS LCD ) to DNA-binding transcription factor FLI1. The resulting fusion, EWS::FLI1, directly interacts with engages in a dynamic interplay that drives tumorigenesis regulates function both proteins. While known promote self-association, role RNA-binding domains (RBDs) EWS, which include RGG repeat regions structured RNA-recognition motif (RRM), remains less...
Abstract The FET family proteins, which includes FUS, EWS, and TAF15, are RNA chaperones instrumental in processes such as mRNA maturation, transcriptional regulation, the DNA damage response. These proteins have clinical significance: chromosomal rearrangements implicated Ewing tumors related sarcomas. Furthermore, point mutations FUS TAF15 associated with neurodegenerative conditions like amyotrophic lateral sclerosis frontotemporal lobar dementia. fusion protein EWS::FLI1, causative...
Interprotein interactions between the partially unfolded states of γD-crystallin (γD-crys) protein are known to cause cataracts. Therefore, understanding unfolding pathways native γD-crys is extremely crucial delineate their aggregation mechanism. In this study, we have performed extensive all-atom Molecular Dynamics simulations with explicit solvent understand role critical residues that drive stability motifs and domains in its wild type mutant forms. Our findings show while individual not...
Ewing sarcoma RNA-binding protein 1 (EWS) is a member of the FET (FUS, EWS, TAF15) family RNA binding proteins that function in transcription, splicing, and DNA damage repair. Mutations are linked to various neurodegenerative diseases, including frontal temporal dementia amyotrophic lateral sclerosis, participate oncogenic fusions cause numerous pediatric malignancies. like all members, harbors extensive intrinsically disordered regions lack defined structure facilitate multivalent highly...