In this work, the eletrochemiluminescence (ECL) behavior of Cu2+/cysteine complexes and N-(aminobutyl)-N-(ethylisoluminol) (ABEI) functionalized gold nanoparticles combined with chitosan (Cu2+-Cys-ABEI-GNPs-CS) were studied by cyclic voltammetry a double-step potential, which exhibited excellent ECL properties without any coreactant. It was found that intensity Cu2+-Cys-ABEI-GNPs-CS could increase at least 1 order magnitude compared Cu2+-Cys-ABEI-GNPs. Furthermore, coreactant-free label-free...

A new "one-pot" hydrothermal method was developed for the preparation of electrochemiluminescence (ECL) nanoluminophores nanographene oxide wrapping titanium dioxide (nGO@TiO2 NLPs). The characterization demonstrated that nGO@TiO2 NLPs possessed a core–shell-like shape. exhibited potential-resolved ECL property in neutral aqueous solution using K2S2O8 as coreactant. On this basis, label-free ratiometric aptasensor designed. were used to fabricate interface target recognition, signal...

Rhizosphere microorganisms are the main factors affecting formation of high quality medicinal materials and promoting accumulation secondary metabolites. However, composition, diversity, function rhizosphere microbial communities in endangered wild cultivated Rhizoma Atractylodis Macrocephalae (RAM) their relationships with active component have remained unclear. In this study, high-throughput sequencing correlation analysis were used to study community diversity (bacteria fungi) three RAM...

Most electrochemiluminescence (ECL) studies involve single luminophore with a unique emission process, which severely limits its applications. Recently, multicolor ECL has attracted considerable interests. Herein, we report novel nanoluminophore prepared by coating 5,10,15,20-tetrakis(4-carboxyphenyl)-porphyrin (TCPP) and N-(4-aminobutyl)-N-ethylisoluminol (ABEI) on the surface of TiO2 nanoparticles (TiO2-TCPP-ABEI), exhibited potential-resolved emissions using H2O2 K2S2O8 as coreactants in...

A novel electrochemiluminescence (ECL) immunosensor was developed for the determination of N-terminal pro-brain natriuretic peptide (NT-proBNP) by using N-(aminobutyl)-N-(ethylisoluminol) (ABEI)-functionalized gold nanodots/chitosan/multiwalled carbon nanotubes (ABEI/GNDs/chitosan/COOH-MWCNTs) hybrid as nanointerface. First, ABEI/GNDs/chitosan/COOH-MWCNTs nanomaterials were grafted onto surface ITO electrode via film-forming property nanomaterials. The anti-NT-proBNP antibody connected to...

N-aminobutyl-N-ethylisoluminol and horseradish peroxidase bifunctionalized graphene oxide hybrids (ABEI-GO@HRP) were prepared through a facile green strategy for the first time. The exhibited excellent chemiluminescence (CL) activity over wide range of pH from 6.1 to 13.0 when reacted with H2O2, whereas ABEI functionalized GO had no CL emission at neutral showed more than 2 orders magnitude lower intensity ABEI-GO@HRP 13.0. Such strong was probably due that HRP facilitated formation O2(•-),...

In this study, allyl isothiocyanate (AITC) was prepared as the dry powder inhalation by loading cyclodextrin metal-organic framework (CD-MOF) to enhance pulmonary delivery. β-CD-MOF and γ-CD-MOF both could be used carry AITC with optimal conditions (50˚C, nCD: nAITC = 1:7, 7 h). Compared β-CD-MOF, had more advantages in due its high drug stable crystal morphology. The particle size mass median aerodynamic diameter of γ-CD-MOF-AITC were accorded characteristics lung inhalation. might...

In this work, three different kinds of acridinium ester (AE)-functionalized carbon nanomaterials, including AE-functionalized nanoparticles (AE-CNPs), graphene oxide (AE-GO), and multiwalled nanotubes (AE-MCNTs), were synthesized for the first time via a simple, general, noncovalent strategy. AE molecules assembled on surface nanomaterials by electrostatic interaction, π–π stacking amide bond. The AE-CNPs, AE-GO, AE-MCNTs with 5.0 × 10–8 mol·L–1 synthetic concentration, which was very low...

Chronic obstructive pulmonary disease (COPD) is a progressive inflammatory condition, and its clinical management primarily targets bronchodilation anti-inflammatory therapy. However, these treatments often fail to directly address the progression of COPD, particularly associated glucocorticoid (GC) resistance. This study elucidates mechanisms underlying GC resistance in COPD explores therapeutic potential allyl isothiocyanate (AITC) modulating MRP1 transport. We assessed levels oxidative...

Chronic obstructive pulmonary disease (COPD) is an important healthcare problem worldwide. Often, glucocorticoid (GC) resistance develops during COPD treatment. As a classic hypoglycemic drug, metformin (MET) can be used as treatment strategy for due to its anti-inflammatory and antioxidant effects, but specific mechanism of action not known. We aimed clarify the role MET on cigarette smoke extract (CSE)-induced GC resistance. Through establishment model in rats, we found that could improve...

Long-term and constant-release osmotic-pump lorcaserin hydrochloride controlled-release tablets (OP LH CRTs) were prepared, to investigate the influencing factors of release optimize formulation. The mechanism from OP CRTs in vitro was investigated. By establishing a high-efficiency method for measuring vitro, optimizing it by single-factor orthogonal experiments, best formulation determined. Then, optimal prescription was: = 20.8 mg; mannitol 100 mg, microcrystalline cellulose 125 magnesium...

The search for novel efficient organic electrochemiluminescence (ECL) emitters is still an active and ongoing topic. Herein, a well-known metal-organic semiconducting charge transfer structure, AgTCNQ, identified as promising ECL emitter. AgTCNQ nanoshuttles were synthesized used the first time to demonstrate robust cathodic emission with K2S2O8 coreactant. mechanism of was investigated, revealing red-shifted attributed surface states. Leveraging strong stable nanoshuttles, label-free...

Pharmacological studies have shown that the rhizome of Atractylodes macrocephala Koidz. (Compositae), commonly known as atractylodes (AMR), can modulate immunity. Nevertheless, its resources been largely depleted, and pharmacological activity artificial AMR is relatively modest. We hypothesized fermented crude extracts rhizosphere fungi would similar immunomodulatory effects since metabolites generated by these are to those host plant given their long-term synergistic evolution.

This paper mainly introduces the application of PLC simulation control in industrial production, and requires use configuration software (SIMEINS S7-200) system connection, lower computer implementation, host monitoring method to build complete assembly line system. Through confi guration monitoring, this implements monitoring.

Gold nanoclusters (AuNCs), as a category of promising electrochemiluminescence (ECL) emitter, hold great potential for sensing applications. However, its application is still restricted by the relatively low ECL quantum yield and difficult to purification. In this work, AuNCs were confined in soft chitosan nanogel matrix with good biocompatibility via facile one-pot approach. The as-fabricated AuNCs@CS nanogels showed 2.4-fold intensity relative that bare without chitosan. enhancement was...

In this study, allyl isothiocyanate (AITC) was prepared as dry powder inhalation by loading cyclodextrin metal-organic framework (CD-MOF) to masking its undesirable flavor, increasing stability and enhancing pulmonary delivery. β-CD-MOF γ-CD-MOF could both be used carry AITC with the optimal conditions (50˚C, nCD-MOF: nAITC = 1:7, 7h). Compared β-CD-MOF, had more advantages in due high drug stable crystal morphology. The particle size mass median aerodynamic diameter of γ-CD-MOF-AITC were...