A5079469867- interferon and immune responses
- Inflammasome and immune disorders
- RNA modifications and cancer
- Atherosclerosis and Cardiovascular Diseases
- CRISPR and Genetic Engineering
- Cytomegalovirus and herpesvirus research
- Systemic Lupus Erythematosus Research
- Chromosomal and Genetic Variations
- Amoebic Infections and Treatments
- Pigment Synthesis and Properties
- Parasitic Infections and Diagnostics
- Corneal Surgery and Treatments
- Extracellular vesicles in disease
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Phytochemistry and Bioactivity Studies
- Ocular Infections and Treatments
- RNA Research and Splicing
- Sphingolipid Metabolism and Signaling
- Viral Infectious Diseases and Gene Expression in Insects
- RNA and protein synthesis mechanisms
- Antifungal resistance and susceptibility
- Autoimmune and Inflammatory Disorders Research
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Ubiquitin and proteasome pathways
Medical Research Foundation
2025
Aravind Eye Hospital
2025
University of Virginia
2017-2021
University of Kentucky
2015-2017
Abstract Cyclic guanosine monophosphate-adenosine monophosphate (cGAMP), produced by cyclic GMP-AMP synthase (cGAS), stimulates the production of type I interferons (IFN). Here we show that cGAMP activates DNA damage response (DDR) signaling independently its canonical IFN pathways. Loss cGAS dampens DDR induced genotoxic insults. Mechanistically, in a STING-TBK1-dependent manner, wherein TBK1 autophosphorylation kinase ATM, with consequent activation CHK2-p53-p21 signal transduction pathway...
Significance Alu elements, comprising more than 10% of the human genome, propagate via retrotransposition. This genomic expansion requires enzymatic activity L1 that reverse transcribes RNA into cDNA in nucleus. We report also undergoes L1-mediated transcription self-priming cytoplasm independent retrotransposition, providing evidence DNA synthesis this cellular compartment. newly discovered shunt molecule replication cycle induces death retinal pigmented epithelium, a hallmark atrophic...
Long interspersed nuclear element-1 (L1)–mediated reverse transcription (RT) of
ObjectiveThe objective of this study was to develop a rapid and accurate CRISPR/Cas12a-based molecular diagnostic assay (Rapid Identification Mycoses using CRISPR, RID-MyC Assay) detect fungal nucleic acids compare it with existing conventional mycologic methods for the diagnosis keratitis (FK).DesignThis structured as development validation focusing on creation assessment novel modality FK.SubjectsParticipants comprised 142 individuals presenting suspected microbial at three tertiary care...
The Prp43 DExD/H-box protein is required for progression of the biochemically distinct pre-messenger RNA and ribosomal (rRNA) maturation pathways. In Saccharomyces cerevisiae, Spp382/Ntr1, Sqs1/Pfa1, Pxr1/Gno1 proteins are implicated as cofactors necessary helicase activation during spliceosome dissociation (Spp382) rRNA processing (Sqs1 Pxr1). While otherwise dissimilar in primary sequence, these Prp43-binding each contain a short glycine-rich G-patch motif function thought to act or...
The level of insulin-like growth factor-binding protein 1 (IGFBP1), a liver-produced serum that regulates factor-I bioactivity, glucose homeostasis, and tissue regeneration, increases during inflammation. This manuscript describes novel pathway for the regulation hepatic IGFBP1 mRNA levels by interleukin (IL)-1β. Experiments with luciferase reporter system show IL-1β stimulates transcriptional activity from 1-kb promoter region IGFBP1. Although stimulation suppresses insulin activation...