A5022927415- Plant Molecular Biology Research
- Light effects on plants
- Plant Gene Expression Analysis
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Plant Stress Responses and Tolerance
Temasek Life Sciences Laboratory
2023-2025
National University of Singapore
2023-2025
Auxin, particularly indole-3-acetic acid (IAA), is a phytohormone critical for plant growth, development, and response to environmental stresses like shade avoidance syndrome thermomorphogenesis. Despite its importance, there no existing method that allows convenient direct detection of IAA in various species. Here, we introduce near-infrared fluorescent nanosensor directly measures planta using corona phase molecular recognition with high selectivity, specificity, spatiotemporal resolution....
Abstract During de-etiolation, dark-grown seedlings are exposed to light, which triggers chlorophyll biosynthesis and greening of the cotyledons. LONG HYPOCOTYL IN FAR-RED 1 (HFR1) is known interact with PHYTOCHROME INTERACTING FACTORs (PIFs) regulate many light-mediated developmental processes in Arabidopsis. Here, we found that overexpressing HFR1 [HFR1(ΔN)-OE] showed photo-oxidative bleaching reduced during similar pif1-1. To elucidate role regulating de-etiolation greening, transcriptome...
Vegetative shade causes an array of morphological changes in plants called avoidance syndrome, which includes hypocotyl and petiole elongation, leaf hyponasty, reduced growth, early flowering rapid senescence. Here, we show that loss-of-function mutations HISTONE DEACETYLASE 9 (HDA9) attenuated the shade-induced elongation Arabidopsis. However, hda9 cotyledons petioles under were not significantly different from those wild-type, suggesting a specific function HDA9 response to shade....
Abstract Auxins, particularly indole-3-acetic acid (IAA), is a phytohormone critical for plant growth, development, and response to environmental stimuli. Despite its importance, there lack of species-independent sensors that allow direct reversible detection IAA. Herein, we introduce novel near infrared fluorescent nanosensor spatial temporal measurement IAA in planta using Corona Phase Molecular Recognition. The shows high specificity vitro was validated localize function cells. sensor...
Plants respond to vegetative shade with developmental and physiological changes that are collectively known as avoidance syndrome (SAS). Although LONG HYPOCOTYL IN FAR-RED 1 (HFR1) is be a negative regulator of SAS by forming heterodimers other basic helix-loop-helix (bHLH) transcription factors inhibit them, its function in genome-wide transcriptional regulation has not been fully elucidated. Here, we performed RNA-sequencing analyses Arabidopsis thaliana hfr1-5 mutant HFR1 overexpression...
Light is crucial for plants and serves as a signal modulating their growth. Under shade, where red to far-red light ratio low, exhibit shade avoidance responses (SAR).